Mouse Anti-MAPRE1 Recombinant Antibody (1A11/4) (CBMAB-E2187-FY)

Name: Mouse Anti-MAPRE1 Recombinant Antibody (1A11/4)
SKU: CBMAB-E2187-FY
Rating: 5 (1 reviews)

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Datasheet

SDS

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Datasheet Target References Q & As Review & reward Protocols Associated Products

Basic Information

Host Animal

Mouse

Clone

1A11/4

Application

WB, IP

Specificity

Human, Monkey

Antibody Isotype

IgG2b

Clonality

Monoclonal

Application Notes

The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Formulations & Storage [For reference only, actual COA shall prevail!]

Format

Liquid

Storage

Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

More Infomation

Target

Full Name

Microtubule Associated Protein RP/EB Family Member 1

Introduction

The protein encoded by this gene was first identified by its binding to the APC protein which is often mutated in familial and sporadic forms of colorectal cancer. This protein localizes to microtubules, especially the growing ends, in interphase cells. During mitosis, the protein is associated with the centrosomes and spindle microtubules. The protein also associates with components of the dynactin complex and the intermediate chain of cytoplasmic dynein. Because of these associations, it is thought that this protein is involved in the regulation of microtubule structures and chromosome stability. This gene is a member of the RP/EB family.

Entrez Gene ID

Human	22919
Monkey	711404

UniProt ID

Human	Q15691
Monkey	G7N533

Alternative Names

Microtubule Associated Protein RP/EB Family Member 1; Adenomatous Polyposis Coli-Binding Protein EB1; APC-Binding Protein EB1; End-Binding Protein 1; EB1; Microtubule-Associated Protein RP/EB Family Member 1

Function

Plus-end tracking protein (+TIP) that binds to the plus-end of microtubules and regulates the dynamics of the microtubule cytoskeleton (PubMed:12388762, PubMed:16109370, PubMed:19632184, PubMed:21646404, PubMed:23001180, PubMed:28726242, PubMed:28814570, PubMed:34608293).
Promotes cytoplasmic microtubule nucleation and elongation (PubMed:12388762, PubMed:16109370, PubMed:19632184, PubMed:21646404, PubMed:28726242, PubMed:28814570).
Involved in mitotic spindle positioning by stabilizing microtubules and promoting dynamic connection between astral microtubules and the cortex during mitotic chromosome segregation (PubMed:12388762, PubMed:34608293).
Also acts as a regulator of minus-end microtubule organization: interacts with the complex formed by AKAP9 and PDE4DIP, leading to recruit CAMSAP2 to the Golgi apparatus, thereby tethering non-centrosomal minus-end microtubules to the Golgi, an important step for polarized cell movement (PubMed:28814570).
Promotes elongation of CAMSAP2-decorated microtubule stretches on the minus-end of microtubules (PubMed:28814570).
Acts as a regulator of autophagosome transport via interaction with CAMSAP2 (PubMed:28726242).
May play a role in cell migration (By similarity).

Biological Process

Attachment of mitotic spindle microtubules to kinetochoreManual Assertion Based On ExperimentIDA:UniProtKB
Cell divisionIEA:UniProtKB-KW
Cell migrationManual Assertion Based On ExperimentIMP:ARUK-UCL
Establishment of mitotic spindle orientationManual Assertion Based On ExperimentIDA:UniProtKB
Microtubule bundle formationIEA:Ensembl
Microtubule polymerizationIEA:Ensembl
Negative regulation of microtubule polymerizationManual Assertion Based On ExperimentIDA:UniProtKB
Non-motile cilium assemblyIEA:Ensembl
Positive regulation of microtubule plus-end bindingIEA:Ensembl
Positive regulation of microtubule polymerizationManual Assertion Based On ExperimentIMP:UniProtKB
Protein localizationManual Assertion Based On ExperimentTAS:ARUK-UCL
Protein localization to astral microtubuleManual Assertion Based On ExperimentIDA:UniProtKB
Protein localization to centrosomeIEA:Ensembl
Protein localization to microtubuleManual Assertion Based On ExperimentIDA:UniProtKB
Protein localization to microtubule plus-endManual Assertion Based On ExperimentIBA:GO_Central
Regulation of microtubule polymerization or depolymerizationManual Assertion Based On ExperimentIBA:GO_Central
Spindle assemblyManual Assertion Based On ExperimentIBA:GO_Central

Cellular Location

Cytoplasm, cytoskeleton
Cytoplasm, cytoskeleton, microtubule organizing center, centrosome
Golgi apparatus
Cytoplasm, cytoskeleton, spindle
Cytoplasm, cytoskeleton, spindle pole
Associated with the microtubule growing distal tips (PubMed:28814570).
Recruitment to the Golgi apparatus requires the presence of PDE4DIP isoform 13/MMG8/SMYLE (PubMed:25217626).

PTM

Acetylation at Lys-220 by KAT2B/PCAF promotes dynamic kinetochore-microtubule interactions in early mitosis.
Crotonylated by KAT5 during mitosis, promoting astral microtubule plasticity and dynamic connection between astral microtubules and the cortex during mitotic chromosome segregation, thereby ensuring accurate spindle positioning in mitosis (PubMed:34608293).
Decrotonylated by HDAC3 (PubMed:34608293).

Li, D., Li, X., He, H., Zhang, Y., He, H., Sun, C., ... & Cui, C. (2022). miR-10a-5p inhibits chicken granulosa cells proliferation and Progesterone (P4) synthesis by targeting MAPRE1 to suppress CDK2. Theriogenology, 192, 97-108.

Qiu, L., Xing, S., Lang, F., & Bao, Q. (2022). MiR-526b-3p inhibits the resistance of glioma cells to adriamycin by targeting MAPRE1. Journal of Oncology, 2022.

Tang, L., Xiong, W., Zhang, L., Wang, D., Wang, Y., Wu, Y., ... & Zeng, Z. (2021). circSETD3 regulates MAPRE1 through miR-615-5p and miR-1538 sponges to promote migration and invasion in nasopharyngeal carcinoma. Oncogene, 40(2), 307-321.

Liang, X. H., Feng, Z. P., Liu, F. Q., Yan, R., Yin, L. Y., Shen, H., & Lu, H. L. (2020). MAPRE1 promotes cell cycle progression of hepatocellular carcinoma cells by interacting with CDK2. Cell Biology International, 44(11), 2326-2333.

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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme-Linked Immunospot (ELISpot)
Proteogenomics
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Isotype control

For research use only. Not intended for any clinical use.

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