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Mouse Anti-MAPRE1 Recombinant Antibody (EB1-CL 1A11/4) (CBMAB-E0023-FY)

This product is mouse antibody that recognizes MAPRE1. The antibody EB1-CL 1A11/4 can be used for immunoassay techniques such as: WB, ELISA, IHC.
See all MAPRE1 antibodies

Summary

Host Animal
Mouse
Specificity
Human, Dog
Clone
EB1-CL 1A11/4
Antibody Isotype
IgG2b
Application
WB, ELISA, IHC

Basic Information

Specificity
Human, Dog
Antibody Isotype
IgG2b
Clonality
Monoclonal
Application Notes
The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Formulations & Storage [For reference only, actual COA shall prevail!]

Format
Liquid
Storage
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

Target

Full Name
Microtubule Associated Protein RP/EB Family Member 1
Introduction
The protein encoded by this gene was first identified by its binding to the APC protein which is often mutated in familial and sporadic forms of colorectal cancer. This protein localizes to microtubules, especially the growing ends, in interphase cells. During mitosis, the protein is associated with the centrosomes and spindle microtubules. The protein also associates with components of the dynactin complex and the intermediate chain of cytoplasmic dynein. Because of these associations, it is thought that this protein is involved in the regulation of microtubule structures and chromosome stability. This gene is a member of the RP/EB family.
Entrez Gene ID
Human22919
Dog100685707
UniProt ID
HumanQ15691
Alternative Names
Microtubule Associated Protein RP/EB Family Member 1; Adenomatous Polyposis Coli-Binding Protein EB1; APC-Binding Protein EB1; End-Binding Protein 1; EB1; Microtubule-Associated Protein RP/EB Family Member 1
Function
Plus-end tracking protein (+TIP) that binds to the plus-end of microtubules and regulates the dynamics of the microtubule cytoskeleton (PubMed:12388762, PubMed:16109370, PubMed:19632184, PubMed:21646404, PubMed:23001180, PubMed:28726242, PubMed:28814570, PubMed:34608293).
Promotes cytoplasmic microtubule nucleation and elongation (PubMed:12388762, PubMed:16109370, PubMed:19632184, PubMed:21646404, PubMed:28726242, PubMed:28814570).
Involved in mitotic spindle positioning by stabilizing microtubules and promoting dynamic connection between astral microtubules and the cortex during mitotic chromosome segregation (PubMed:12388762, PubMed:34608293).
Also acts as a regulator of minus-end microtubule organization: interacts with the complex formed by AKAP9 and PDE4DIP, leading to recruit CAMSAP2 to the Golgi apparatus, thereby tethering non-centrosomal minus-end microtubules to the Golgi, an important step for polarized cell movement (PubMed:28814570).
Promotes elongation of CAMSAP2-decorated microtubule stretches on the minus-end of microtubules (PubMed:28814570).
Acts as a regulator of autophagosome transport via interaction with CAMSAP2 (PubMed:28726242).
May play a role in cell migration (By similarity).
Biological Process
Attachment of mitotic spindle microtubules to kinetochoreManual Assertion Based On ExperimentIDA:UniProtKB
Cell divisionIEA:UniProtKB-KW
Cell migrationManual Assertion Based On ExperimentIMP:ARUK-UCL
Establishment of mitotic spindle orientationManual Assertion Based On ExperimentIDA:UniProtKB
Microtubule bundle formationIEA:Ensembl
Microtubule polymerizationIEA:Ensembl
Negative regulation of microtubule polymerizationManual Assertion Based On ExperimentIDA:UniProtKB
Non-motile cilium assemblyIEA:Ensembl
Positive regulation of microtubule plus-end bindingIEA:Ensembl
Positive regulation of microtubule polymerizationManual Assertion Based On ExperimentIMP:UniProtKB
Protein localizationManual Assertion Based On ExperimentTAS:ARUK-UCL
Protein localization to astral microtubuleManual Assertion Based On ExperimentIDA:UniProtKB
Protein localization to centrosomeIEA:Ensembl
Protein localization to microtubuleManual Assertion Based On ExperimentIDA:UniProtKB
Protein localization to microtubule plus-endManual Assertion Based On ExperimentIBA:GO_Central
Regulation of microtubule polymerization or depolymerizationManual Assertion Based On ExperimentIBA:GO_Central
Spindle assemblyManual Assertion Based On ExperimentIBA:GO_Central
Cellular Location
Cytoplasm, cytoskeleton
Cytoplasm, cytoskeleton, microtubule organizing center, centrosome
Golgi apparatus
Cytoplasm, cytoskeleton, spindle
Cytoplasm, cytoskeleton, spindle pole
Associated with the microtubule growing distal tips (PubMed:28814570).
Recruitment to the Golgi apparatus requires the presence of PDE4DIP isoform 13/MMG8/SMYLE (PubMed:25217626).
PTM
Acetylation at Lys-220 by KAT2B/PCAF promotes dynamic kinetochore-microtubule interactions in early mitosis.
Crotonylated by KAT5 during mitosis, promoting astral microtubule plasticity and dynamic connection between astral microtubules and the cortex during mitotic chromosome segregation, thereby ensuring accurate spindle positioning in mitosis (PubMed:34608293).
Decrotonylated by HDAC3 (PubMed:34608293).
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For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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