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MAPRE1

The protein encoded by this gene was first identified by its binding to the APC protein which is often mutated in familial and sporadic forms of colorectal cancer. This protein localizes to microtubules, especially the growing ends, in interphase cells. During mitosis, the protein is associated with the centrosomes and spindle microtubules. The protein also associates with components of the dynactin complex and the intermediate chain of cytoplasmic dynein. Because of these associations, it is thought that this protein is involved in the regulation of microtubule structures and chromosome stability. This gene is a member of the RP/EB family. [provided by RefSeq, Jul 2008]
Full Name
Microtubule Associated Protein RP/EB Family Member 1
Function
Plus-end tracking protein (+TIP) that binds to the plus-end of microtubules and regulates the dynamics of the microtubule cytoskeleton (PubMed:12388762, PubMed:16109370, PubMed:19632184, PubMed:21646404, PubMed:23001180, PubMed:28726242, PubMed:28814570, PubMed:34608293).
Promotes cytoplasmic microtubule nucleation and elongation (PubMed:12388762, PubMed:16109370, PubMed:19632184, PubMed:21646404, PubMed:28726242, PubMed:28814570).
Involved in mitotic spindle positioning by stabilizing microtubules and promoting dynamic connection between astral microtubules and the cortex during mitotic chromosome segregation (PubMed:12388762, PubMed:34608293).
Also acts as a regulator of minus-end microtubule organization: interacts with the complex formed by AKAP9 and PDE4DIP, leading to recruit CAMSAP2 to the Golgi apparatus, thereby tethering non-centrosomal minus-end microtubules to the Golgi, an important step for polarized cell movement (PubMed:28814570).
Promotes elongation of CAMSAP2-decorated microtubule stretches on the minus-end of microtubules (PubMed:28814570).
Acts as a regulator of autophagosome transport via interaction with CAMSAP2 (PubMed:28726242).
May play a role in cell migration (By similarity).
Biological Process
Attachment of mitotic spindle microtubules to kinetochoreManual Assertion Based On ExperimentIDA:UniProtKB
Cell divisionIEA:UniProtKB-KW
Cell migrationManual Assertion Based On ExperimentIMP:ARUK-UCL
Establishment of mitotic spindle orientationManual Assertion Based On ExperimentIDA:UniProtKB
Microtubule bundle formationIEA:Ensembl
Microtubule polymerizationIEA:Ensembl
Negative regulation of microtubule polymerizationManual Assertion Based On ExperimentIDA:UniProtKB
Non-motile cilium assemblyIEA:Ensembl
Positive regulation of microtubule plus-end bindingIEA:Ensembl
Positive regulation of microtubule polymerizationManual Assertion Based On ExperimentIMP:UniProtKB
Protein localizationManual Assertion Based On ExperimentTAS:ARUK-UCL
Protein localization to astral microtubuleManual Assertion Based On ExperimentIDA:UniProtKB
Protein localization to centrosomeIEA:Ensembl
Protein localization to microtubuleManual Assertion Based On ExperimentIDA:UniProtKB
Protein localization to microtubule plus-endManual Assertion Based On ExperimentIBA:GO_Central
Regulation of microtubule polymerization or depolymerizationManual Assertion Based On ExperimentIBA:GO_Central
Spindle assemblyManual Assertion Based On ExperimentIBA:GO_Central
Cellular Location
Cytoplasm, cytoskeleton
Cytoplasm, cytoskeleton, microtubule organizing center, centrosome
Golgi apparatus
Cytoplasm, cytoskeleton, spindle
Cytoplasm, cytoskeleton, spindle pole
Associated with the microtubule growing distal tips (PubMed:28814570).
Recruitment to the Golgi apparatus requires the presence of PDE4DIP isoform 13/MMG8/SMYLE (PubMed:25217626).
PTM
Acetylation at Lys-220 by KAT2B/PCAF promotes dynamic kinetochore-microtubule interactions in early mitosis.
Crotonylated by KAT5 during mitosis, promoting astral microtubule plasticity and dynamic connection between astral microtubules and the cortex during mitotic chromosome segregation, thereby ensuring accurate spindle positioning in mitosis (PubMed:34608293).
Decrotonylated by HDAC3 (PubMed:34608293).

Anti-MAPRE1 antibodies

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Target: MAPRE1
Host: Mouse
Antibody Isotype: IgG2a, κ
Specificity: Human
Clone: 4F3
Application*: SE, E
Target: MAPRE1
Host: Mouse
Antibody Isotype: IgG2a, κ
Specificity: Human
Clone: CBFYM-0567
Application*: E
Target: MAPRE1
Host: Rat
Antibody Isotype: IgG2a
Specificity: Hamster, Human, Mouse
Clone: CBFYM-1669
Application*: E, IF, IP, WB
Target: MAPRE1
Host: Rat
Antibody Isotype: IgG2a
Specificity: Human, Mouse, Monkey
Clone: KT51
Application*: WB, IF
Target: MAPRE1
Host: Mouse
Antibody Isotype: IgG2b
Specificity: Human, Monkey
Clone: 1A11/4
Application*: WB, IP
Target: MAPRE1
Host: Mouse
Antibody Isotype: IgG2b
Specificity: Human, Dog
Clone: EB1-CL 1A11/4
Application*: WB, E, IH
Target: MAPRE1
Host: Mouse
Antibody Isotype: IgG1
Specificity: Human, Chicken, Mouse, Rat
Clone: CBFYE-1545
Application*: WB, IF, P
For Research Use Only. Not For Clinical Use.
(P): Predicted
* Abbreviations
IFImmunofluorescence
IHImmunohistochemistry
IPImmunoprecipitation
WBWestern Blot
EELISA
MMicroarray
CIChromatin Immunoprecipitation
FFlow Cytometry
FNFunction Assay
IDImmunodiffusion
RRadioimmunoassay
TCTissue Culture
GSGel Supershift
NNeutralization
BBlocking
AActivation
IInhibition
DDepletion
ESELISpot
DBDot Blot
MCMass Cytometry/CyTOF
CTCytotoxicity
SStimulation
AGAgonist
APApoptosis
IMImmunomicroscopy
BABioassay
CSCostimulation
EMElectron Microscopy
IEImmunoelectrophoresis
PAPeptide Array
ICImmunocytochemistry
PEPeptide ELISA
MDMeDIP
SHIn situ hybridization
IAEnzyme Immunoassay
SEsandwich ELISA
PLProximity Ligation Assay
ECELISA(Cap)
EDELISA(Det)
BIBioimaging
IOImmunoassay
LFLateral Flow Immunoassay
LALuminex Assay
CImmunohistochemistry-Frozen Sections
PImmunohistologyp-Paraffin Sections
ISIntracellular Staining for Flow Cytometry
MSElectrophoretic Mobility Shift Assay
RIRNA Binding Protein Immunoprecipitation (RIP)
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