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Mouse Anti-MARCKS Recombinant Antibody (2H4) (CBMAB-A5303-LY)

The product is antibody recognizes MARCKS. The antibody 2H4 immunoassay techniques such as: WB, ELISA.
See all MARCKS antibodies

Summary

Host Animal
Mouse
Specificity
Human, Mouse
Clone
2H4
Antibody Isotype
IgG1, κ
Application
WB, ELISA

Basic Information

Immunogen
MARCKS (NP_002347, 2 a.a. ~ 65 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
Specificity
Human, Mouse
Antibody Isotype
IgG1, κ
Clonality
Monoclonal
Application Notes
The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Formulations & Storage [For reference only, actual COA shall prevail!]

Format
Liquid
Purity
> 95% Purity determined by SDS-PAGE.
Storage
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freezethaw cycles.

Target

Full Name
Myristoylated Alanine Rich Protein Kinase C Substrate
Introduction
The protein encoded by this gene is a substrate for protein kinase C. It is localized to the plasma membrane and is an actin filament crosslinking protein. Phosphorylation by protein kinase C or binding to calcium-calmodulin inhibits its association with actin and with the plasma membrane, leading to its presence in the cytoplasm. The protein is thought to be involved in cell motility, phagocytosis, membrane trafficking and mitogenesis. [provided by RefSeq]
Entrez Gene ID
Human4082
Mouse17118
UniProt ID
HumanP29966
MouseP26645
Alternative Names
80K-L; FLJ14368; FLJ90045; MACS; PKCSL; PRKCSL
Function
MARCKS is the most prominent cellular substrate for protein kinase C. This protein binds calmodulin, actin, and synapsin. MARCKS is a filamentous (F) actin cross-linking protein.
Biological Process
Actin crosslink formation Source: Ensembl
Actin filament bundle assembly Source: Ensembl
Actin filament organization Source: GO_Central
Central nervous system development Source: GO_Central
Cellular Location
Cytoskeleton
Other locations
Membrane
PTM
Phosphorylation by PKC displaces MARCKS from the membrane. It also inhibits the F-actin cross-linking activity.

Fouedji, C., Sylvin Etémé, A., Tabi, C. B., & Fouda, H. P. E. (2023). Pattern formation in a one-dimensional MARCKS protein cyclic model with spatially inhomogeneous diffusion coefficients. The European Physical Journal Plus, 138(11), 1-10.

Manai, M., ELBini-Dhouib, I., Finetti, P., Bichiou, H., Reduzzi, C., Aissaoui, D., ... & Bertucci, F. (2022). MARCKS as a Potential Therapeutic Target in Inflammatory Breast Cancer. Cells, 11(18), 2926.

Chen, Z., Zhang, W., Selmi, C., Ridgway, W. M., Leung, P. S., Zhang, F., & Gershwin, M. E. (2021). The myristoylated alanine-rich C-kinase substrates (MARCKS): A membrane-anchored mediator of the cell function. Autoimmunity reviews, 20(11), 102942.

Iyer, D. N., Faruq, O., Zhang, L., Rastgoo, N., Liu, A., & Chang, H. (2021). Pathophysiological roles of myristoylated alanine-rich C-kinase substrate (MARCKS) in hematological malignancies. Biomarker Research, 9(1), 34.

Liu, J., Chen, S. J., Hsu, S. W., Zhang, J., Li, J. M., Yang, D. C., ... & Chen, C. H. (2021). MARCKS cooperates with NKAP to activate NF-kB signaling in smoke-related lung cancer. Theranostics, 11(9), 4122.

Beckmann, L., Berg, V., Dickhut, C., Sun, C., Merkel, O., Bloehdorn, J., ... & Frenzel, L. P. (2021). MARCKS affects cell motility and response to BTK inhibitors in CLL. Blood, The Journal of the American Society of Hematology, 138(7), 544-556.

Mohapatra, P., Yadav, V., Toftdahl, M., & Andersson, T. (2020). WNT5A-induced activation of the protein kinase C substrate MARCKS is required for melanoma cell invasion. Cancers, 12(2), 346.

Sheats, M. K., Yin, Q., Fang, S., Park, J., Crews, A. L., Parikh, I., ... & Adler, K. B. (2019). MARCKS and lung disease. American Journal of Respiratory Cell and Molecular Biology, 60(1), 16-27.

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For research use only. Not intended for any clinical use.

Custom Antibody Labeling

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