Mouse Anti-MARCKS (AA 2-66) Recombinant Antibody (CBFYM-1707) (CBMAB-M1874-FY)

Name: Mouse Anti-MARCKS (AA 2-66) Recombinant Antibody (CBFYM-1707)
SKU: CBMAB-M1874-FY
Rating: 5 (1 reviews)

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Datasheet

SDS

Request for COA

Datasheet Target References Q & As Review & reward Protocols Associated Products

Basic Information

Host Animal

Mouse

Clone

CBFYM-1707

Application

ELISA, IF, IHC-P, WB

Immunogen

Recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.

Specificity

Human, Mouse

Antibody Isotype

IgG1, k

Clonality

Monoclonal

Application Notes

The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Formulations & Storage [For reference only, actual COA shall prevail!]

Format

Liquid

Buffer

PBS, pH 7.2

Concentration

0.43 mg/mL

Storage

Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

Epitope

AA 2-66

More Infomation

Target

Full Name

Myristoylated Alanine Rich Protein Kinase C Substrate

Introduction

The protein encoded by this gene is a substrate for protein kinase C. It is localized to the plasma membrane and is an actin filament crosslinking protein. Phosphorylation by protein kinase C or binding to calcium-calmodulin inhibits its association with actin and with the plasma membrane, leading to its presence in the cytoplasm. The protein is thought to be involved in cell motility, phagocytosis, membrane trafficking and mitogenesis.

Entrez Gene ID

Human	4082
Mouse	17118

UniProt ID

Human	P29966
Mouse	P26645

Alternative Names

Myristoylated Alanine Rich Protein Kinase C Substrate; Myristoylated Alanine-Rich Protein Kinase C Substrate (MARCKS, 80K-L); Protein Kinase C Substrate, 80 KDa Protein, Light Chain; PRKCSL; PKCSL; MACS

Function

MARCKS is the most prominent cellular substrate for protein kinase C. This protein binds calmodulin, actin, and synapsin. MARCKS is a filamentous (F) actin cross-linking protein.

Biological Process

Actin crosslink formation Source: Ensembl
Actin filament bundle assembly Source: Ensembl
Actin filament organization Source: GO_Central
Central nervous system development Source: GO_Central

Cellular Location

Cytoskeleton
Other locations
Membrane

PTM

Phosphorylation by PKC displaces MARCKS from the membrane. It also inhibits the F-actin cross-linking activity.

Fouedji, C., Sylvin Etémé, A., Tabi, C. B., & Fouda, H. P. E. (2023). Pattern formation in a one-dimensional MARCKS protein cyclic model with spatially inhomogeneous diffusion coefficients. The European Physical Journal Plus, 138(11), 1-10.

Manai, M., ELBini-Dhouib, I., Finetti, P., Bichiou, H., Reduzzi, C., Aissaoui, D., ... & Bertucci, F. (2022). MARCKS as a Potential Therapeutic Target in Inflammatory Breast Cancer. Cells, 11(18), 2926.

Chen, Z., Zhang, W., Selmi, C., Ridgway, W. M., Leung, P. S., Zhang, F., & Gershwin, M. E. (2021). The myristoylated alanine-rich C-kinase substrates (MARCKS): A membrane-anchored mediator of the cell function. Autoimmunity reviews, 20(11), 102942.

Iyer, D. N., Faruq, O., Zhang, L., Rastgoo, N., Liu, A., & Chang, H. (2021). Pathophysiological roles of myristoylated alanine-rich C-kinase substrate (MARCKS) in hematological malignancies. Biomarker Research, 9(1), 34.

Liu, J., Chen, S. J., Hsu, S. W., Zhang, J., Li, J. M., Yang, D. C., ... & Chen, C. H. (2021). MARCKS cooperates with NKAP to activate NF-kB signaling in smoke-related lung cancer. Theranostics, 11(9), 4122.

Beckmann, L., Berg, V., Dickhut, C., Sun, C., Merkel, O., Bloehdorn, J., ... & Frenzel, L. P. (2021). MARCKS affects cell motility and response to BTK inhibitors in CLL. Blood, The Journal of the American Society of Hematology, 138(7), 544-556.

Mohapatra, P., Yadav, V., Toftdahl, M., & Andersson, T. (2020). WNT5A-induced activation of the protein kinase C substrate MARCKS is required for melanoma cell invasion. Cancers, 12(2), 346.

Sheats, M. K., Yin, Q., Fang, S., Park, J., Crews, A. L., Parikh, I., ... & Adler, K. B. (2019). MARCKS and lung disease. American Journal of Respiratory Cell and Molecular Biology, 60(1), 16-27.

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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme Linked Immunospot (ELISpot)
Proteogenomic
Other Protocols

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Human MARCKS ELISA Kit (CAT#: V2LY-0626-LY4925)

Isotype control

For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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