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Mouse Anti-MARK3 (AA 402-500) Recombinant Antibody (CBFYM-1721) (CBMAB-M1888-FY)

This product is mouse antibody that recognizes MARK3. The antibody CBFYM-1721 can be used for immunoassay techniques such as: ELISA, WB.
See all MARK3 antibodies

Summary

Host Animal
Mouse
Specificity
Human
Clone
CBFYM-1721
Antibody Isotype
IgG2b, k
Application
ELISA, WB

Basic Information

Immunogen
Recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.Immunogen sequence: HHKVQRSVFS SQKQRRYSDH AGPAIPSVVA YPKRSQTSTA DSDLKEDGIS SRKSSGSAVG GKGIAPASPM LGNASNPNKA DIPERKKSST VPSSNTASG
Specificity
Human
Antibody Isotype
IgG2b, k
Clonality
Monoclonal
Application Notes
The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Formulations & Storage [For reference only, actual COA shall prevail!]

Format
Liquid
Storage
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
Epitope
AA 402-500

Target

Full Name
MAP/microtubule affinity-regulating kinase 3
Introduction
The protein encoded by this gene is activated by phosphorylation and in turn is involved in the phosphorylation of tau proteins MAP2 and MAP4. Several transcript variants encoding different isoforms have been found for this gene.
Entrez Gene ID
4140
UniProt ID
P27448
Alternative Names
Microtubule Affinity Regulating Kinase 3; MAP/Microtubule Affinity-Regulating Kinase 3; Serine/Threonine-Protein Kinase P78; Cdc25C-Associated Protein Kinase 1; Ser/Thr Protein Kinase PAR-1; Protein Kinase STK10; ELKL Motif Kinase 2; EC 2.7.11.1; C-TAK1
Function
Serine/threonine-protein kinase (PubMed:23666762).

Involved in the specific phosphorylation of microtubule-associated proteins for MAP2 and MAP4. Phosphorylates the microtubule-associated protein MAPT/TAU (PubMed:23666762).

Phosphorylates CDC25C on 'Ser-216'. Regulates localization and activity of some histone deacetylases by mediating phosphorylation of HDAC7, promoting subsequent interaction between HDAC7 and 14-3-3 and export from the nucleus (PubMed:16980613).

Negatively regulates the Hippo signaling pathway and antagonizes the phosphorylation of LATS1. Cooperates with DLG5 to inhibit the kinase activity of STK3/MST2 toward LATS1 (PubMed:28087714).
Biological Process
Intracellular signal transduction Source: GO_Central
Microtubule cytoskeleton organization Source: GO_Central
Negative regulation of hippo signaling Source: UniProtKB
Peptidyl-serine autophosphorylation Source: ARUK-UCL
Peptidyl-serine phosphorylation Source: ARUK-UCL
Positive regulation of protein binding Source: ARUK-UCL
Protein phosphorylation Source: UniProtKB
Cellular Location
Plasma membrane
Cell membrane
Cytoplasm and Cytosol
Cytoplasm
Other locations
Dendrite
Involvement in disease
Visual impairment and progressive phthisis bulbi (VIPB):
An autosomal recessive, progressive disease characterized by poor vision at birth and development of bilateral phthisis bulbi by adulthood.
PTM
Phosphorylated at Thr-211 by STK11/LKB1 in complex with STE20-related adapter-alpha (STRADA) pseudo kinase and CAB39. Phosphorylation at Thr-564 by PRKCZ/aPKC inhibits the kinase activity.

Wang, L., Liu, X., & Xia, Z. (2023). CircUBXN7 Impedes Apoptosis to Alleviate Myocardial Infarction Injury by Regulating the miR-582-3p/MARK3 Axis. Annals of Clinical & Laboratory Science, 53(2), 303-312.

Yang, J., Niu, H., Pang, S., Liu, M., Chen, F., Li, Z., ... & Huang, Y. (2023). MARK3 kinase: Regulation and physiologic roles. Cellular Signalling, 103, 110578.

Baqai, U., Kurimchak, A. M., Trachtenberg, I. V., Purwin, T. J., Haj, J. I., Han, A., ... & Aplin, A. E. (2023). Kinome profiling identifies MARK3 and STK10 as potential therapeutic targets in uveal melanoma. Journal of Biological Chemistry, 299(12).

Mishima, T., Ohta, Y., Ohashi, K., & Mizuno, K. (2023). MARK3-mediated Slingshot-1 phosphorylation is essential for polarized lamellipodium formation. bioRxiv, 2023-10.

Machino, H., Kaneko, S., Komatsu, M., Ikawa, N., Asada, K., Nakato, R., ... & Hamamoto, R. (2022). The metabolic stress-activated checkpoint LKB1-MARK3 axis acts as a tumor suppressor in high-grade serous ovarian carcinoma. Communications Biology, 5(1), 39.

Zhang, Q., Mesner, L. D., Calabrese, G. M., Dirckx, N., Li, Z., Verardo, A., ... & Clemens, T. L. (2021). Genomic variants within chromosome 14q32. 32 regulate bone mass through MARK3 signaling in osteoblasts. The Journal of Clinical Investigation, 131(7).

Li, F., Liu, Z., Sun, H., Li, C., Wang, W., Ye, L., ... & Wang, H. (2020). PCC0208017, a novel small-molecule inhibitor of MARK3/MARK4, suppresses glioma progression in vitro and in vivo. Acta Pharmaceutica Sinica B, 10(2), 289-300.

Jeon, T. E., & Jin, H. S. (2019). PDGFC, MARK3 and BCL2 Polymorphisms are Associated with Left Ventricular Hypertrophy in Korean Population. Biomedical Science Letters, 25(3), 237-246.

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For research use only. Not intended for any clinical use.

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