Sign in or Register   Sign in or Register
  |  

Mouse Anti-MBD3 Antibody (2C4) (CBMAB-0640-YC)

Provided herein are mouse monoclonal antibodies against Human MBD3. The antibody clone 2C4 can be used for immunoassay techniques, such as ELISA, IP and WB.
See all MBD3 antibodies

Summary

Host Animal
Mouse
Specificity
Human
Clone
2C4
Antibody Isotype
IgG1
Application
ELISA, IP, WB

Basic Information

Immunogen
Recombinant protein
Specificity
Human
Antibody Isotype
IgG1
Application Notes
The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Formulations & Storage [For reference only, actual COA shall prevail!]

Format
Supernatant
Storage
Store at 4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

Target

Full Name
Methyl-CpG Binding Domain Protein 3
Introduction
MBD3 (methyl-CpG binding domain protein 3) belongs to a family of nuclear proteins which are characterized by the presence of a methyl-CpG binding domain (MBD). MBD3 is a subunit of the NuRD, a multisubunit complex containing nucleosome remodeling and histone deacetylase activities. MBD3 is not capable of binding to methylated DNA and mediates the association of metastasis-associated protein 2 with the core histone deacetylase complex.
Entrez Gene ID
53615
UniProt ID
O95983
Alternative Names
Methyl-CpG binding domain protein 3
Function
Acts as transcriptional repressor and plays a role in gene silencing. Does not bind to DNA by itself (PubMed:12124384).

Binds to DNA with a preference for sites containing methylated CpG dinucleotides (in vitro). Binds to a lesser degree DNA containing unmethylated CpG dinucleotides (PubMed:24307175).

Recruits histone deacetylases and DNA methyltransferases.
Biological Process
Aging Source: Ensembl
Brain development Source: Ensembl
Chromatin remodeling Source: ComplexPortal
DNA methylation-dependent heterochromatin assembly Source: Ensembl
Embryonic organ development Source: Ensembl
Heart development Source: Ensembl
Histone acetylation Source: Ensembl
Histone deacetylation Source: ComplexPortal
In utero embryonic development Source: Ensembl
Negative regulation of transcription, DNA-templated Source: ComplexPortal
Negative regulation of transcription by RNA polymerase II Source: Ensembl
Positive regulation of transcription, DNA-templated Source: ComplexPortal
Regulation of cell fate specification Source: ComplexPortal
Regulation of DNA methylation Source: Ensembl
Regulation of stem cell differentiation Source: ComplexPortal
Response to estradiol Source: Ensembl
Response to nutrient levels Source: Ensembl
Tissue development Source: Ensembl
Cellular Location
Nucleus
Other locations
Chromosome
Note: Nuclear, in discrete foci. Detected on chromatin, at promoter regions of active genes.

Leighton, G. O., Irvin, E. M., Kaur, P., Liu, M., You, C., Bhattaram, D., ... & Williams, D. C. (2022). Densely methylated DNA traps methyl-CpG–binding domain protein 2 but permits free diffusion by methyl-CpG–binding domain protein 3. Journal of Biological Chemistry, 298(10).

Wang, H., Feng, Y., Sun, J., Zhang, W., Han, Z., Yu, S., ... & Na, M. (2021). Methyl-CpG-binding domain protein 3 promotes seizures by recruiting methyltransferase DNMT1 to enhance TREM2 methylation. Neurochemical Research, 46(9), 2451-2462.

Ishibashi, T., Kajihara, I., Mizuhashi, S., Kuriyama, H., Kimura, T., Kanemaru, H., ... & Ihn, H. (2020). Methyl-CpG binding domain protein 3: a new diagnostic marker and potential therapeutic target of melanoma. BioScience Trends, 14(5), 390-395.

Dai, Y., Li, J., Li, M., Liu, Z., Liu, J., An, L., & Du, F. (2020). Methyl-CpG-binding domain 3 (Mbd3) is an important regulator for apoptosis in mouse embryonic stem cells. American journal of translational research, 12(12), 8147.

Leighton, G., & Williams Jr, D. C. (2020). The methyl-CpG–Binding domain 2 and 3 proteins and formation of the nucleosome remodeling and deacetylase complex. Journal of molecular biology, 432(6), 1624-1639.

Wang, H., Feng, W., Chen, W., He, J., Min, J., Liu, Y., ... & Xu, M. (2020). Methyl-CpG-binding domain 3 inhibits stemness of pancreatic cancer cells via Hippo signaling. Experimental Cell Research, 393(1), 112091.

Song, X., Zhang, Y., Zhong, Q., Zhan, K., Bi, J., Tang, J., ... & Li, B. (2020). Identification and functional characterization of methyl-CpG binding domain protein from Tribolium castaneum. Genomics, 112(3), 2223-2232.

Liu, K., Lei, M., Wu, Z., Gan, B., Cheng, H., Li, Y., & Min, J. (2019). Structural analyses reveal that MBD 3 is a methylated CG binder. The FEBS Journal, 286(16), 3240-3254.

Ask a question We look forward to hearing from you.
0 reviews or Q&As

Purchasing FAQs
Technical FAQs

Loading...
Have you used Mouse Anti-MBD3 Antibody (2C4)?
Submit a review and get a Coupon or an Amazon gift card. 20% off Coupon $30 eGift Card
Submit a review
Loading...
For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

Online Inquiry

Documents

Contact us

  • Tel: (USA)
  • (UK)
  • Fax:
  • Email:

Submit A Review

Go to
Compare