Mouse Anti-MBD3 Antibody (2C4) (CBMAB-0640-YC)

Name: Mouse Anti-MBD3 Antibody (2C4)
SKU: CBMAB-0640-YC
Rating: 5 (1 reviews)

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Datasheet

SDS

Request for COA

Datasheet Target References Q & As Review & reward Protocols Associated Products

Basic Information

Host Animal

Mouse

Clone

2C4

Application

ELISA, IP, WB

Immunogen

Recombinant protein

Specificity

Human

Antibody Isotype

IgG1

Application Notes

The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Formulations & Storage [For reference only, actual COA shall prevail!]

Format

Supernatant

Storage

Store at 4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

More Infomation

Target

Full Name

Methyl-CpG Binding Domain Protein 3

Introduction

MBD3 (methyl-CpG binding domain protein 3) belongs to a family of nuclear proteins which are characterized by the presence of a methyl-CpG binding domain (MBD). MBD3 is a subunit of the NuRD, a multisubunit complex containing nucleosome remodeling and histone deacetylase activities. MBD3 is not capable of binding to methylated DNA and mediates the association of metastasis-associated protein 2 with the core histone deacetylase complex.

Entrez Gene ID

53615

UniProt ID

O95983

Alternative Names

Methyl-CpG binding domain protein 3

Function

Acts as transcriptional repressor and plays a role in gene silencing. Does not bind to DNA by itself (PubMed:12124384).

Binds to DNA with a preference for sites containing methylated CpG dinucleotides (in vitro). Binds to a lesser degree DNA containing unmethylated CpG dinucleotides (PubMed:24307175).

Recruits histone deacetylases and DNA methyltransferases.

Biological Process

Aging Source: Ensembl
Brain development Source: Ensembl
Chromatin remodeling Source: ComplexPortal
DNA methylation-dependent heterochromatin assembly Source: Ensembl
Embryonic organ development Source: Ensembl
Heart development Source: Ensembl
Histone acetylation Source: Ensembl
Histone deacetylation Source: ComplexPortal
In utero embryonic development Source: Ensembl
Negative regulation of transcription, DNA-templated Source: ComplexPortal
Negative regulation of transcription by RNA polymerase II Source: Ensembl
Positive regulation of transcription, DNA-templated Source: ComplexPortal
Regulation of cell fate specification Source: ComplexPortal
Regulation of DNA methylation Source: Ensembl
Regulation of stem cell differentiation Source: ComplexPortal
Response to estradiol Source: Ensembl
Response to nutrient levels Source: Ensembl
Tissue development Source: Ensembl

Cellular Location

Nucleus
Other locations
Chromosome
Note: Nuclear, in discrete foci. Detected on chromatin, at promoter regions of active genes.

Leighton, G. O., Irvin, E. M., Kaur, P., Liu, M., You, C., Bhattaram, D., ... & Williams, D. C. (2022). Densely methylated DNA traps methyl-CpG–binding domain protein 2 but permits free diffusion by methyl-CpG–binding domain protein 3. Journal of Biological Chemistry, 298(10).

Wang, H., Feng, Y., Sun, J., Zhang, W., Han, Z., Yu, S., ... & Na, M. (2021). Methyl-CpG-binding domain protein 3 promotes seizures by recruiting methyltransferase DNMT1 to enhance TREM2 methylation. Neurochemical Research, 46(9), 2451-2462.

Ishibashi, T., Kajihara, I., Mizuhashi, S., Kuriyama, H., Kimura, T., Kanemaru, H., ... & Ihn, H. (2020). Methyl-CpG binding domain protein 3: a new diagnostic marker and potential therapeutic target of melanoma. BioScience Trends, 14(5), 390-395.

Dai, Y., Li, J., Li, M., Liu, Z., Liu, J., An, L., & Du, F. (2020). Methyl-CpG-binding domain 3 (Mbd3) is an important regulator for apoptosis in mouse embryonic stem cells. American journal of translational research, 12(12), 8147.

Leighton, G., & Williams Jr, D. C. (2020). The methyl-CpG–Binding domain 2 and 3 proteins and formation of the nucleosome remodeling and deacetylase complex. Journal of molecular biology, 432(6), 1624-1639.

Wang, H., Feng, W., Chen, W., He, J., Min, J., Liu, Y., ... & Xu, M. (2020). Methyl-CpG-binding domain 3 inhibits stemness of pancreatic cancer cells via Hippo signaling. Experimental Cell Research, 393(1), 112091.

Song, X., Zhang, Y., Zhong, Q., Zhan, K., Bi, J., Tang, J., ... & Li, B. (2020). Identification and functional characterization of methyl-CpG binding domain protein from Tribolium castaneum. Genomics, 112(3), 2223-2232.

Liu, K., Lei, M., Wu, Z., Gan, B., Cheng, H., Li, Y., & Min, J. (2019). Structural analyses reveal that MBD 3 is a methylated CG binder. The FEBS Journal, 286(16), 3240-3254.

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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme Linked Immunospot (ELISpot)
Proteogenomic
Other Protocols

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Isotype control

For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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