Rabbit Anti-MBD3 Recombinant Antibody (CBFYM-0318) (CBMAB-M0383-FY)

Name: Rabbit Anti-MBD3 Recombinant Antibody (CBFYM-0318)
SKU: CBMAB-M0383-FY
Rating: 5 (1 reviews)

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Datasheet

SDS

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Basic Information

Host Animal

Rabbit

Clone

CBFYM-0318

Application

WB, IP, FC, IF

Specificity

Mouse, Rat, Cattle, Human

Antibody Isotype

IgG

Application Notes

The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Formulations & Storage [For reference only, actual COA shall prevail!]

Format

Liquid

Storage

Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

More Infomation

Target

Full Name

Methyl-CpG Binding Domain Protein 3

Introduction

DNA methylation is the major modification of eukaryotic genomes and plays an essential role in mammalian development. This gene belongs to a family of nuclear proteins which are characterized by the presence of a methyl-CpG binding domain. The encoded protein is a subunit of the NuRD, a multisubunit complex containing nucleosome remodeling and histone deacetylase activities. Unlike the other family members, the encoded protein is not capable of binding to methylated DNA. The protein mediates the association of metastasis-associated protein 2 with the core histone deacetylase complex. Alternative splicing results in multiple transcript variants of this gene.

Entrez Gene ID

Human	53615
Mouse	17192
Rat	362834
Cattle	616090

UniProt ID

Human	O95983
Mouse	Q9Z2D8
Rat	F7EY92
Cattle	A2VDN2

Alternative Names

Methyl-CpG Binding Domain Protein 3; Methyl-CpG-Binding Protein MBD3

Function

Acts as transcriptional repressor and plays a role in gene silencing. Does not bind to DNA by itself (PubMed:12124384).

Binds to DNA with a preference for sites containing methylated CpG dinucleotides (in vitro). Binds to a lesser degree DNA containing unmethylated CpG dinucleotides (PubMed:24307175).

Recruits histone deacetylases and DNA methyltransferases.

Biological Process

Aging Source: Ensembl
Brain development Source: Ensembl
Chromatin remodeling Source: ComplexPortal
DNA methylation-dependent heterochromatin assembly Source: Ensembl
Embryonic organ development Source: Ensembl
Heart development Source: Ensembl
Histone acetylation Source: Ensembl
Histone deacetylation Source: ComplexPortal
In utero embryonic development Source: Ensembl
Negative regulation of transcription, DNA-templated Source: ComplexPortal
Negative regulation of transcription by RNA polymerase II Source: Ensembl
Positive regulation of transcription, DNA-templated Source: ComplexPortal
Regulation of cell fate specification Source: ComplexPortal
Regulation of DNA methylation Source: Ensembl
Regulation of stem cell differentiation Source: ComplexPortal
Response to estradiol Source: Ensembl
Response to nutrient levels Source: Ensembl
Tissue development Source: Ensembl

Cellular Location

Nucleus
Other locations
Chromosome
Note: Nuclear, in discrete foci. Detected on chromatin, at promoter regions of active genes.

Leighton, G. O., Irvin, E. M., Kaur, P., Liu, M., You, C., Bhattaram, D., ... & Williams, D. C. (2022). Densely methylated DNA traps methyl-CpG–binding domain protein 2 but permits free diffusion by methyl-CpG–binding domain protein 3. Journal of Biological Chemistry, 298(10).

Wang, H., Feng, Y., Sun, J., Zhang, W., Han, Z., Yu, S., ... & Na, M. (2021). Methyl-CpG-binding domain protein 3 promotes seizures by recruiting methyltransferase DNMT1 to enhance TREM2 methylation. Neurochemical Research, 46(9), 2451-2462.

Ishibashi, T., Kajihara, I., Mizuhashi, S., Kuriyama, H., Kimura, T., Kanemaru, H., ... & Ihn, H. (2020). Methyl-CpG binding domain protein 3: a new diagnostic marker and potential therapeutic target of melanoma. BioScience Trends, 14(5), 390-395.

Dai, Y., Li, J., Li, M., Liu, Z., Liu, J., An, L., & Du, F. (2020). Methyl-CpG-binding domain 3 (Mbd3) is an important regulator for apoptosis in mouse embryonic stem cells. American journal of translational research, 12(12), 8147.

Leighton, G., & Williams Jr, D. C. (2020). The methyl-CpG–Binding domain 2 and 3 proteins and formation of the nucleosome remodeling and deacetylase complex. Journal of molecular biology, 432(6), 1624-1639.

Wang, H., Feng, W., Chen, W., He, J., Min, J., Liu, Y., ... & Xu, M. (2020). Methyl-CpG-binding domain 3 inhibits stemness of pancreatic cancer cells via Hippo signaling. Experimental Cell Research, 393(1), 112091.

Song, X., Zhang, Y., Zhong, Q., Zhan, K., Bi, J., Tang, J., ... & Li, B. (2020). Identification and functional characterization of methyl-CpG binding domain protein from Tribolium castaneum. Genomics, 112(3), 2223-2232.

Liu, K., Lei, M., Wu, Z., Gan, B., Cheng, H., Li, Y., & Min, J. (2019). Structural analyses reveal that MBD 3 is a methylated CG binder. The FEBS Journal, 286(16), 3240-3254.

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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme Linked Immunospot (ELISpot)
Proteogenomic
Other Protocols

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Isotype control

For research use only. Not intended for any clinical use.

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We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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