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Mouse Anti-MCF2L Recombinant Antibody (1D11) (CBMAB-A5340-LY)

The product is antibody recognizes MCF2L. The antibody 1D11 immunoassay techniques such as: WB, ELISA.
See all MCF2L antibodies
Published Data
Fig. 1 MCF2L689Gly does not activate Rac1. The MCF2L-689Gly variant does not activate Rac1. Hela cells were transiently transfected with wild-type MCF2L689Asp or the mutant MCF2L689Gly constructs. Rac1 protein was measured in cell lysates. Rac1-GTP was measured as described in methods section. Wild-type MCF2L689Asp but not MCF2L689Gly activates endogenous Rac1. Beta-actin was used as a loading control.
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Summary

Host Animal
Mouse
Specificity
Human
Clone
1D11
Antibody Isotype
IgG1, κ
Application
WB, ELISA

Basic Information

Immunogen
MCF2L (AAH20208, 220 a.a. ~ 319 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
Specificity
Human
Antibody Isotype
IgG1, κ
Clonality
Monoclonal
Application Notes
The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Formulations & Storage [For reference only, actual COA shall prevail!]

Format
Liquid
Purity
> 95% Purity determined by SDS-PAGE.
Storage
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freezethaw cycles.

Target

Full Name
MCF.2 cell line derived transforming sequence-like
Entrez Gene ID
23263
UniProt ID
O15068
Alternative Names
ARHGEF14; DBS; FLJ12122; KIAA0362; OST
Function
Guanine nucleotide exchange factor that catalyzes guanine nucleotide exchange on RHOA and CDC42, and thereby contributes to the regulation of RHOA and CDC42 signaling pathways (By similarity).

Seems to lack activity with RAC1. Becomes activated and highly tumorigenic by truncation of the N-terminus (By similarity).

Isoform 5 activates CDC42 (PubMed:15157669).

Isoform 3:
Does not catalyze guanine nucleotide exchange on CDC42 (PubMed:15157669).
Biological Process
Intracellular signal transduction Source: InterPro
Positive regulation of Rho protein signal transduction Source: UniProtKB
Regulation of small GTPase mediated signal transduction Source: Reactome
Cellular Location
Isoform 5:
Plasma membrane
Cell membrane
Cytoplasm
Isoform 3:
Cytoplasm
Other locations
Endomembrane system
Note: Interaction with membranes enriched in phosphoinositides is mediated by the CRAL-TRIO domain.
Plasma membrane
Cell membrane
Cytoplasm

Ou, H., Qian, Y., & Ma, L. (2023). MCF2L-AS1 promotes the biological behaviors of hepatocellular carcinoma cells by regulating the miR-33a-5p/FGF2 axis. Aging (Albany NY), 15(13), 6100.

Zhu, Y., Yang, L., Wang, J., Li, Y., & Chen, Y. (2022). SP1-induced lncRNA MCF2L-AS1 promotes cisplatin resistance in ovarian cancer by regulating IGF2BP1/IGF2/MEK/ERK axis. Journal of Gynecologic Oncology, 33(6).

She, Q., Chen, Y., Liu, H., Tan, J., & Li, Y. (2022). A high level of the long non-coding RNA MCF2L-AS1 is associated with poor prognosis in breast cancer and MCF2L-AS1 activates YAP transcriptional activity to enhance breast cancer proliferation and metastasis. Bioengineered, 13(5), 13437-13451.

Huang, F. K., Zheng, C. Y., Huang, L. K., Lin, C. Q., Zhou, J. F., & Wang, J. X. (2021). Long non‐coding RNA MCF2L‐AS1 promotes the aggressiveness of colorectal cancer by sponging miR‐874‐3p and thereby up‐regulating CCNE1. The Journal of Gene Medicine, 23(1), e3285.

Li, S., & Lin, L. (2021). Long noncoding RNA MCF2L‐AS1 promotes the cancer stem cell‐like traits in non‐small cell lung cancer cells through regulating miR‐873‐5p level. Environmental Toxicology, 36(7), 1457-1465.

Zhang, Z., Yang, W., Li, N., Chen, X., Ma, F., Yang, J., ... & Hua, Y. (2021). LncRNA MCF2L-AS1 aggravates proliferation, invasion and glycolysis of colorectal cancer cells via the crosstalk with miR-874-3p/FOXM1 signaling axis. Carcinogenesis, 42(2), 263-271.

Kong, W., Li, H., Xie, L., Cui, G., Gu, W., Zhang, H., ... & Zhou, Y. (2021). LncRNA MCF2L-AS1 aggravates the malignant development of colorectal cancer via targeting miR-105-5p/RAB22A axis. BMC cancer, 21, 1-13.

Cai, M., Hu, W., Huang, C., Zhou, C., Li, J., Chen, Y., & Yu, Y. (2021). lncRNA MCF2L-AS1/miR-105/IL-1β axis regulates colorectal cancer cell oxaliplatin resistance. Cancer Management and Research, 8685-8694.

Mao, J. H., Sui, Y. X., Ao, S., Wang, Y., Liu, Y., & Leng, H. (2020). miR-140-3p exhibits repressive functions on preosteoblast viability and differentiation by downregulating MCF2L in osteoporosis. In Vitro Cellular & Developmental Biology-Animal, 56(1), 49-58.

Chen, Q., Wang, M., & Wu, S. (2020). The lncRNA MCF2L-AS1 controls osteogenic differentiation by regulating miR-33a. Cell Cycle, 19(9), 1059-1065.

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For research use only. Not intended for any clinical use.

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