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Mouse Anti-MCF2L Recombinant Antibody (CBFYM-1868) (CBMAB-M2039-FY)

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Datasheet
SDS
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Datasheet Target References Q & As Review & reward Protocols Associated Products

Basic Information

Host Animal
Mouse
Clone
CBFYM-1868
Application
ELISA, WB, IHC-P
Specificity
Human
Antibody Isotype
IgG1, k
Application Notes
The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Formulations & Storage [For reference only, actual COA shall prevail!]

Format
Liquid
Buffer
PBS, pH 7.2
Storage
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
More Infomation

Target

Full Name
MCF.2 cell line derived transforming sequence-like
Introduction
This gene encodes a guanine nucleotide exchange factor that interacts specifically with the GTP-bound Rac1 and plays a role in the Rho/Rac signaling pathways. A variant in this gene was associated with osteoarthritis. Alternative splicing results in multiple transcript variants.
Entrez Gene ID
23263
UniProt ID
O15068
Alternative Names
MCF.2 Cell Line Derived Transforming Sequence Like; DBLs Big Sister; OST; MCF2 Transforming Sequence-Like Protein; MCF2-Transforming Sequence-Like Protein; Guanine Nucleotide Exchange Factor DBS; ARHGEF14; KIAA0362; DBS
Function
Guanine nucleotide exchange factor that catalyzes guanine nucleotide exchange on RHOA and CDC42, and thereby contributes to the regulation of RHOA and CDC42 signaling pathways (By similarity).

Seems to lack activity with RAC1. Becomes activated and highly tumorigenic by truncation of the N-terminus (By similarity).

Isoform 5 activates CDC42 (PubMed:15157669).

Isoform 3:
Does not catalyze guanine nucleotide exchange on CDC42 (PubMed:15157669).
Biological Process
Intracellular signal transduction Source: InterPro
Positive regulation of Rho protein signal transduction Source: UniProtKB
Regulation of small GTPase mediated signal transduction Source: Reactome
Cellular Location
Isoform 5:
Plasma membrane
Cell membrane
Cytoplasm
Isoform 3:
Cytoplasm
Other locations
Endomembrane system
Note: Interaction with membranes enriched in phosphoinositides is mediated by the CRAL-TRIO domain.
Plasma membrane
Cell membrane
Cytoplasm

Ou, H., Qian, Y., & Ma, L. (2023). MCF2L-AS1 promotes the biological behaviors of hepatocellular carcinoma cells by regulating the miR-33a-5p/FGF2 axis. Aging (Albany NY), 15(13), 6100.

Zhu, Y., Yang, L., Wang, J., Li, Y., & Chen, Y. (2022). SP1-induced lncRNA MCF2L-AS1 promotes cisplatin resistance in ovarian cancer by regulating IGF2BP1/IGF2/MEK/ERK axis. Journal of Gynecologic Oncology, 33(6).

She, Q., Chen, Y., Liu, H., Tan, J., & Li, Y. (2022). A high level of the long non-coding RNA MCF2L-AS1 is associated with poor prognosis in breast cancer and MCF2L-AS1 activates YAP transcriptional activity to enhance breast cancer proliferation and metastasis. Bioengineered, 13(5), 13437-13451.

Huang, F. K., Zheng, C. Y., Huang, L. K., Lin, C. Q., Zhou, J. F., & Wang, J. X. (2021). Long non‐coding RNA MCF2L‐AS1 promotes the aggressiveness of colorectal cancer by sponging miR‐874‐3p and thereby up‐regulating CCNE1. The Journal of Gene Medicine, 23(1), e3285.

Li, S., & Lin, L. (2021). Long noncoding RNA MCF2L‐AS1 promotes the cancer stem cell‐like traits in non‐small cell lung cancer cells through regulating miR‐873‐5p level. Environmental Toxicology, 36(7), 1457-1465.

Zhang, Z., Yang, W., Li, N., Chen, X., Ma, F., Yang, J., ... & Hua, Y. (2021). LncRNA MCF2L-AS1 aggravates proliferation, invasion and glycolysis of colorectal cancer cells via the crosstalk with miR-874-3p/FOXM1 signaling axis. Carcinogenesis, 42(2), 263-271.

Kong, W., Li, H., Xie, L., Cui, G., Gu, W., Zhang, H., ... & Zhou, Y. (2021). LncRNA MCF2L-AS1 aggravates the malignant development of colorectal cancer via targeting miR-105-5p/RAB22A axis. BMC cancer, 21, 1-13.

Cai, M., Hu, W., Huang, C., Zhou, C., Li, J., Chen, Y., & Yu, Y. (2021). lncRNA MCF2L-AS1/miR-105/IL-1β axis regulates colorectal cancer cell oxaliplatin resistance. Cancer Management and Research, 8685-8694.

Mao, J. H., Sui, Y. X., Ao, S., Wang, Y., Liu, Y., & Leng, H. (2020). miR-140-3p exhibits repressive functions on preosteoblast viability and differentiation by downregulating MCF2L in osteoporosis. In Vitro Cellular & Developmental Biology-Animal, 56(1), 49-58.

Chen, Q., Wang, M., & Wu, S. (2020). The lncRNA MCF2L-AS1 controls osteogenic differentiation by regulating miR-33a. Cell Cycle, 19(9), 1059-1065.

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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme Linked Immunospot (ELISpot)
Proteogenomic
Other Protocols

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Isotype control

For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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