Mouse Anti-MCL1 (AA 1-327) Recombinant Antibody (CBFYM-0790) (CBMAB-M0934-FY)

Name: Mouse Anti-MCL1 (AA 1-327) Recombinant Antibody (CBFYM-0790)
SKU: CBMAB-M0934-FY
Rating: 5 (1 reviews)

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Datasheet

SDS

Request for COA

Datasheet Target References Q & As Review & reward Protocols Associated Products

Basic Information

Host Animal

Mouse

Clone

CBFYM-0790

Application

IP, WB

Immunogen

Recombinant protein

Specificity

Human

Antibody Isotype

IgG1, k

Clonality

Monoclonal

Application Notes

The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Formulations & Storage [For reference only, actual COA shall prevail!]

Format

Liquid

Concentration

0.4 mg/mL

Storage

Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

Epitope

AA 1-327

More Infomation

Target

Full Name

MCL1, BCL2 Family Apoptosis Regulator

Introduction

This gene encodes an anti-apoptotic protein, which is a member of the Bcl-2 family. Alternative splicing results in multiple transcript variants. The longest gene product enhances cell survival by inhibiting apoptosis while the alternatively spliced shorter gene products promote apoptosis and are death-inducing.

Entrez Gene ID

4170

UniProt ID

Q07820

Alternative Names

MCL1, BCL2 Family Apoptosis Regulator; Myeloid Cell Leukemia Sequence 1 (BCL2-Related); Bcl-2-Related Protein EAT/Mcl1; Myeloid Cell Leukemia 1; Bcl-2-Like Protein 3; Bcl2-L-3; Mcl1/EAT; BCL2L3; Induced Myeloid Leukemia Cell Differentiation Protein Mcl-1

Function

Involved in the regulation of apoptosis versus cell survival, and in the maintenance of viability but not of proliferation. Mediates its effects by interactions with a number of other regulators of apoptosis. Isoform 1 inhibits apoptosis. Isoform 2 promotes apoptosis.

Biological Process

Cell fate determination Source: UniProtKB
Cellular homeostasis Source: UniProtKB
Extrinsic apoptotic signaling pathway in absence of ligand Source: UniProtKB
Intrinsic apoptotic signaling pathway in response to DNA damage Source: GO_Central
Negative regulation of anoikis Source: UniProtKB
Negative regulation of apoptotic process Source: BHF-UCL
Negative regulation of autophagy Source: UniProtKB
Negative regulation of extrinsic apoptotic signaling pathway in absence of ligand Source: UniProtKB
Negative regulation of intrinsic apoptotic signaling pathway Source: GO_Central
Positive regulation of apoptotic process Source: ComplexPortal
Positive regulation of oxidative stress-induced neuron intrinsic apoptotic signaling pathway Source: ParkinsonsUK-UCL
Regulation of apoptotic process Source: ComplexPortal
Regulation of response to DNA damage stimulus Source: MGI
Response to cytokine Source: MGI

Cellular Location

Mitochondrion
Cytoplasm
Nucleus
nucleoplasm
Other locations
Membrane
Note: Cytoplasmic, associated with mitochondria.

Topology

Helical: 328-348

PTM

Cleaved by CASP3 during apoptosis. In intact cells cleavage occurs preferentially after Asp-127, yielding a pro-apoptotic 28 kDa C-terminal fragment.
Rapidly degraded in the absence of phosphorylation on Thr-163 in the PEST region.
Phosphorylated on Ser-159, by GSK3, in response to IL3/interleukin-3 withdrawal. Phosphorylation at Ser-159 induces ubiquitination and proteasomal degradation, abrogating the anti-apoptotic activity. Treatment with taxol or okadaic acid induces phosphorylation on additional sites.
Ubiquitinated. Ubiquitination is induced by phosphorylation at Ser-159.

Sancho, M., Leiva, D., Lucendo, E., & Orzáez, M. (2022). Understanding MCL1: from cellular function and regulation to pharmacological inhibition. The FEBS Journal, 289(20), 6209-6234.

Widden, H., & Placzek, W. J. (2021). The multiple mechanisms of MCL1 in the regulation of cell fate. Communications Biology, 4(1), 1029.

Roberts, A. W., Wei, A. H., & Huang, D. C. (2021). BCL2 and MCL1 inhibitors for hematologic malignancies. Blood, The Journal of the American Society of Hematology, 138(13), 1120-1136.

Senichkin, V. V., Streletskaia, A. Y., Gorbunova, A. S., Zhivotovsky, B., & Kopeina, G. S. (2020). Saga of Mcl-1: regulation from transcription to degradation. Cell Death & Differentiation, 27(2), 405-419.

Warren, C. F., Wong-Brown, M. W., & Bowden, N. A. (2019). BCL-2 family isoforms in apoptosis and cancer. Cell death & disease, 10(3), 177.

Yamaguchi, R., Lartigue, L., & Perkins, G. (2019). Targeting Mcl-1 and other Bcl-2 family member proteins in cancer therapy. Pharmacology & therapeutics, 195, 13-20.

Senichkin, V. V., Streletskaia, A. Y., Zhivotovsky, B., & Kopeina, G. S. (2019). Molecular comprehension of Mcl-1: from gene structure to cancer therapy. Trends in Cell Biology, 29(7), 549-562.

Lee, E. F., Harris, T. J., Tran, S., Evangelista, M., Arulananda, S., John, T., ... & Fairlie, W. D. (2019). BCL-XL and MCL-1 are the key BCL-2 family proteins in melanoma cell survival. Cell death & disease, 10(5), 342.

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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme-Linked Immunospot (ELISpot)
Proteogenomics
Other Protocols

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Isotype control

For research use only. Not intended for any clinical use.

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