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Rabbit Anti-MCM3 Recombinant Antibody (CBFYM-1901) (CBMAB-M2073-FY)

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Basic Information

Host Animal
Rabbit
Clone
CBFYM-1901
Application
WB
Specificity
Human, Mouse, Rat, Monkey
Antibody Isotype
IgG
Clonality
Monoclonal
Application Notes
The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Formulations & Storage [For reference only, actual COA shall prevail!]

Format
Liquid
Storage
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
More Infomation

Target

Full Name
minichromosome maintenance complex component 3
Introduction
The protein encoded by this gene is one of the highly conserved mini-chromosome maintenance proteins that are involved in the initiation of eukaryotic genome replication. The hexameric protein complex formed by MCM proteins is a key component of the pre-replication complex and may be involved in the formation of replication forks and in the recruitment of other DNA replication related proteins. This protein is a subunit of the protein complex that consists of MCM2-7. It has been shown to interact directly with MCM5/CDC46. This protein also interacts with and is acetylated by MCM3AP, a chromatin-associated acetyltransferase. The acetylation of this protein inhibits the initiation of DNA replication and cell cycle progression. Two transcript variants encoding different isoforms have been found for this gene.
Entrez Gene ID
Human4172
Mouse17215
Rat316273
Monkey708320
UniProt ID
HumanP25205
MouseP25206
RatD3ZFP4
MonkeyH9FL07
Alternative Names
Minichromosome Maintenance Complex Component 3; DNA Polymerase Alpha Holoenzyme-Associated Protein P1; RLF Subunit Beta; P1-MCM3; P102; MCM3 Minichromosome Maintenance Deficient 3 (S. Cerevisiae); Minichromosome Maintenance Deficient (S. Cerevisiae) 3; MCM3 Minichromosome Maintenance Deficient 3; Replication Licensing Factor, Beta Subunit; Minichromosome Maintenance Deficient 3
Research Area
Epigenetics and Nuclear Signaling
Function
Acts as a component of the MCM2-7 complex (MCM complex) which is the replicative helicase essential for 'once per cell cycle' DNA replication initiation and elongation in eukaryotic cells. Core component of CDC45-MCM-GINS (CMG) helicase, the molecular machine that unwinds template DNA during replication, and around which the replisome is built (PubMed:32453425, PubMed:34694004, PubMed:34700328, PubMed:35585232).
The active ATPase sites in the MCM2-7 ring are formed through the interaction surfaces of two neighboring subunits such that a critical structure of a conserved arginine finger motif is provided in trans relative to the ATP-binding site of the Walker A box of the adjacent subunit. The six ATPase active sites, however, are likely to contribute differentially to the complex helicase activity (PubMed:32453425).
Required for the entry in S phase and for cell division (Probable).
Biological Process
DNA replication initiation
Double-strand break repair via break-induced replication
Mitotic DNA replication initiation
Regulation of DNA-templated DNA replication initiation
Cellular Location
Nucleus
Chromosome
Note: Associated with chromatin before the formation of nuclei and detaches from it as DNA replication progresses.
PTM
Acetylated by MCM3AP.
O-glycosylated (O-GlcNAcylated), in a cell cycle-dependent manner.

Zhou, H., Xiong, Y., Zhang, G., Liu, Z., Li, L., Hou, S., & Zhou, T. (2020). Elevated expression of minichromosome maintenance 3 indicates poor outcomes and promotes G1/S cell cycle progression, proliferation, migration and invasion in colorectal cancer. Bioscience reports, 40(7), BSR20201503.

Yang, Q., Xie, B., Tang, H., Meng, W., Jia, C., Zhang, X., ... & Fu, B. (2019). Minichromosome maintenance 3 promotes hepatocellular carcinoma radioresistance by activating the NF-κB pathway. Journal of Experimental & Clinical Cancer Research, 38(1), 1-12.

Aporowicz, M., Czopnik, P., Kubicka, E., Piotrowska, A., Dziegiel, P., Bolanowski, M., & Domoslawski, P. (2019). Minichromosome maintenance proteins MCM-3, MCM-5, MCM-7, and Ki-67 as proliferative markers in adrenocortical tumors. Anticancer Research, 39(3), 1151-1159.

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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme Linked Immunospot (ELISpot)
Proteogenomic
Other Protocols

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For research use only. Not intended for any clinical use.

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