MCM3

The protein encoded by this gene is one of the highly conserved mini-chromosome maintenance proteins (MCM) that are involved in the initiation of eukaryotic genome replication. The hexameric protein complex formed by MCM proteins is a key component of the pre-replication complex (pre_RC) and may be involved in the formation of replication forks and in the recruitment of other DNA replication related proteins. This protein is a subunit of the protein complex that consists of MCM2-7. It has been shown to interact directly with MCM5/CDC46. This protein also interacts with and is acetylated by MCM3AP, a chromatin-associated acetyltransferase. The acetylation of this protein inhibits the initiation of DNA replication and cell cycle progression. Several transcript variants encoding different isoforms have been found for this gene.

Full Name

minichromosome maintenance complex component 3

Research Area

Epigenetics and Nuclear Signaling

Function

Acts as a component of the MCM2-7 complex (MCM complex) which is the replicative helicase essential for 'once per cell cycle' DNA replication initiation and elongation in eukaryotic cells. Core component of CDC45-MCM-GINS (CMG) helicase, the molecular machine that unwinds template DNA during replication, and around which the replisome is built (PubMed:32453425, PubMed:34694004, PubMed:34700328, PubMed:35585232).
The active ATPase sites in the MCM2-7 ring are formed through the interaction surfaces of two neighboring subunits such that a critical structure of a conserved arginine finger motif is provided in trans relative to the ATP-binding site of the Walker A box of the adjacent subunit. The six ATPase active sites, however, are likely to contribute differentially to the complex helicase activity (PubMed:32453425).
Required for the entry in S phase and for cell division (Probable).

Biological Process

DNA replication initiation
Double-strand break repair via break-induced replication
Mitotic DNA replication initiation
Regulation of DNA-templated DNA replication initiation

Cellular Location

Nucleus
Chromosome
Note: Associated with chromatin before the formation of nuclei and detaches from it as DNA replication progresses.

PTM

Acetylated by MCM3AP.
O-glycosylated (O-GlcNAcylated), in a cell cycle-dependent manner.

Anti-MCM3 antibodies

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Rabbit Anti-MCM3 (Phosphorylated S112) Recombinant Antibody (D3S4M) (PTM-CBMAB-0606LY)

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Target: MCM3

Host: Rabbit

Antibody Isotype: IgG

Specificity: Human, Mouse, Rat, Hamster, Zebrafish, Dog

Clone: D3S4M

Application*: WB, IP

Datasheet SDS

Mouse Anti-MCM3 (AA 699-808) Recombinant Antibody (CBFYM-1896) (CBMAB-M2068-FY)

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Target: MCM3

Host: Mouse

Antibody Isotype: IgG2b, κ

Specificity: Human

Clone: CBFYM-1896

Application*: E, IF, WB

Datasheet SDS

Mouse Anti-MCM3 Recombinant Antibody (CBT2085) (V2LY-0625-LY1929)

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Target: MCM3

Host: Mouse

Antibody Isotype: IgG1

Specificity: Human, Monkey, Rat

Clone: CBT2085

Application*: WB, IH, IC, F

Datasheet SDS

Rabbit Anti-MCM3 Recombinant Antibody (D47B6) (CBMAB-CP1476-LY)

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Target: MCM3

Host: Rabbit

Antibody Isotype: IgG

Specificity: Human, Mouse, Rat, Monkey

Clone: D47B6

Application*: WB

Datasheet SDS

Mouse Anti-MCM3 Recombinant Antibody (2H3) (CBMAB-A5347-LY)

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Target: MCM3

Host: Mouse

Antibody Isotype: IgG2a, κ

Specificity: Human

Clone: 2H3

Application*: WB, E

Datasheet SDS

Mouse Anti-MCM3 Recombinant Antibody (3E11) (CBMAB-A5345-LY)

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Target: MCM3

Host: Mouse

Antibody Isotype: IgG2b, κ

Specificity: Human

Clone: 3E11

Application*: WB, E

Datasheet SDS

Mouse Anti-MCM3 Recombinant Antibody (CBFYM-1902) (CBMAB-M2074-FY)

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Target: MCM3

Host: Mouse

Antibody Isotype: IgG

Specificity: Human, Rat, Monkey

Clone: CBFYM-1902

Application*: WB

Datasheet SDS

Rabbit Anti-MCM3 Recombinant Antibody (CBFYM-1901) (CBMAB-M2073-FY)

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Target: MCM3

Host: Rabbit

Antibody Isotype: IgG

Specificity: Human, Mouse, Rat, Monkey

Clone: CBFYM-1901

Application*: WB

Datasheet SDS

Mouse Anti-MCM3 Recombinant Antibody (CBFYM-1900) (CBMAB-M2072-FY)

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Target: MCM3

Host: Mouse

Specificity: Human, Mouse, Rat

Clone: CBFYM-1900

Application*: WB

Datasheet SDS

Mouse Anti-MCM3 (AA 699-808) Recombinant Antibody (CBFYM-1899) (CBMAB-M2071-FY)

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Target: MCM3

Host: Mouse

Antibody Isotype: IgG2a, λ

Specificity: Human, Mouse

Clone: CBFYM-1899

Application*: WB-Ti, WB-Ce, E, WB-Re

Datasheet SDS

Mouse Anti-MCM3 Recombinant Antibody (CBFYM-1898) (CBMAB-M2070-FY)

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Target: MCM3

Host: Mouse

Antibody Isotype: IgG2a

Specificity: Human

Clone: CBFYM-1898

Application*: IC, P, IP, WB

Datasheet SDS

Mouse Anti-MCM3 (AA 26-113) Recombinant Antibody (CBFYM-1897) (CBMAB-M2069-FY)

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Target: MCM3

Host: Mouse

Antibody Isotype: IgG2a, κ

Specificity: Human

Clone: CBFYM-1897

Application*: E, WB

Datasheet SDS

More Infomation

Fig.3 Signaling pathways in cancers. (Creative Biolabs Authorized)

Fig.4 Protocols troubleshootings & guides. (Creative Biolabs Authorized)

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(P): Predicted

* Abbreviations

AActivation
AGAgonist
APApoptosis
BBlocking
BABioassay
BIBioimaging
CImmunohistochemistry-Frozen Sections
CIChromatin Immunoprecipitation
CTCytotoxicity
CSCostimulation
DDepletion
DBDot Blot

EELISA
ECELISA(Cap)
EDELISA(Det)
ESELISpot
EMElectron Microscopy
FFlow Cytometry
FNFunction Assay
GSGel Supershift
IInhibition
IAEnzyme Immunoassay
ICImmunocytochemistry
IDImmunodiffusion

IEImmunoelectrophoresis
IFImmunofluorescence
IHImmunohistochemistry
IMImmunomicroscopy
IOImmunoassay
IPImmunoprecipitation
ISIntracellular Staining for Flow Cytometry
LALuminex Assay
LFLateral Flow Immunoassay
MMicroarray
MCMass Cytometry/CyTOF
MDMeDIP

MSElectrophoretic Mobility Shift Assay
NNeutralization
PImmunohistologyp-Paraffin Sections
PAPeptide Array
PEPeptide ELISA
PLProximity Ligation Assay
RRadioimmunoassay
SStimulation
SESandwich ELISA
SHIn situ hybridization
TCTissue Culture
WBWestern Blot

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