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Mouse Anti-MCM3 Recombinant Antibody (CBT2085) (V2LY-0625-LY1929)


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Summary

Host Animal
Mouse
Specificity
Human, Monkey, Rat
Clone
CBT2085
Antibody Isotype
IgG1
Application
WB, IHC, ICC, FC

Basic Information

Immunogen
Purified recombinant fragment of human MCM3 expressed in E. Coli.
Host Species
Mouse
Specificity
Human, Monkey, Rat
Antibody Isotype
IgG1
Clonality
Monoclonal Antibody
Application Notes
ApplicationNote
WB1:500-1:2,000
IHC-P1:200-1:1,000
ICC1:50-1:200
FC1:200-1:400

Formulations & Storage [For reference only, actual COA shall prevail!]

Format
Liquid
Buffer
PBS
Preservative
Sodium azide
Concentration
Batch dependent
Purity
> 95% Purity determined by SDS-PAGE.
Storage
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freezethaw cycles.

Target

Full Name
minichromosome maintenance complex component 3
Entrez Gene ID
4172
UniProt ID
P25205
Research Area
Epigenetics and Nuclear Signaling
Function
Acts as a component of the MCM2-7 complex (MCM complex) which is the replicative helicase essential for 'once per cell cycle' DNA replication initiation and elongation in eukaryotic cells. Core component of CDC45-MCM-GINS (CMG) helicase, the molecular machine that unwinds template DNA during replication, and around which the replisome is built (PubMed:32453425, PubMed:34694004, PubMed:34700328, PubMed:35585232).
The active ATPase sites in the MCM2-7 ring are formed through the interaction surfaces of two neighboring subunits such that a critical structure of a conserved arginine finger motif is provided in trans relative to the ATP-binding site of the Walker A box of the adjacent subunit. The six ATPase active sites, however, are likely to contribute differentially to the complex helicase activity (PubMed:32453425).
Required for the entry in S phase and for cell division (Probable).
Biological Process
DNA replication initiation
Double-strand break repair via break-induced replication
Mitotic DNA replication initiation
Regulation of DNA-templated DNA replication initiation
Cellular Location
Nucleus
Chromosome
Note: Associated with chromatin before the formation of nuclei and detaches from it as DNA replication progresses.
PTM
Acetylated by MCM3AP.
O-glycosylated (O-GlcNAcylated), in a cell cycle-dependent manner.
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For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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