Sign in or Register   Sign in or Register
  |  

Mouse Anti-MDFI Recombinant Antibody (3B4) (CBMAB-A5356-LY)

The product is antibody recognizes MDFI. The antibody 3B4 immunoassay techniques such as: WB, ELISA.
See all MDFI antibodies

Summary

Host Animal
Mouse
Specificity
Human
Clone
3B4
Antibody Isotype
IgG2a, κ
Application
WB, ELISA

Basic Information

Immunogen
MDFI (NP_005577, 33 a.a. ~ 110 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
Specificity
Human
Antibody Isotype
IgG2a, κ
Clonality
Monoclonal
Application Notes
The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Formulations & Storage [For reference only, actual COA shall prevail!]

Format
Liquid
Purity
> 95% Purity determined by SDS-PAGE.
Storage
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freezethaw cycles.

Target

Full Name
MyoD family inhibitor
Introduction
This protein is a transcription factor that negatively regulates other myogenic family proteins. Studies of the mouse homolog, I-mf, show that it interferes with myogenic factor function by masking nuclear localization signals and preventing DNA binding. Knockout mouse studies show defects in the formation of vertebrae and ribs that also involve cartilage formation in these structures. [provided by RefSeq]
Entrez Gene ID
4188
UniProt ID
Q99750
Alternative Names
I-MF; I-mfa
Function
Inhibits the transactivation activity of the Myod family of myogenic factors and represses myogenesis. Acts by associating with Myod family members and retaining them in the cytoplasm by masking their nuclear localization signals. Can also interfere with the DNA-binding activity of Myod family members. Plays an important role in trophoblast and chondrogenic differentiation. Regulates the transcriptional activity of TCF7L1/TCF3 by interacting directly with TCF7L1/TCF3 and preventing it from binding DNA. Binds to the axin complex, resulting in an increase in the level of free beta-catenin. Affects axin regulation of the WNT and JNK signaling pathways (By similarity).
Biological Process
Cytoplasmic sequestering of transcription factor Source: ProtInc
Dorsal/ventral axis specification Source: UniProtKB
Embryonic skeletal system morphogenesis Source: Ensembl
Negative regulation of DNA binding Source: UniProtKB
Negative regulation of transcription by RNA polymerase II Source: UniProtKB
Negative regulation of Wnt signaling pathway Source: UniProtKB
Trophoblast giant cell differentiation Source: Ensembl
Cellular Location
Nucleus
Cytoplasm

Houser, J. S., Patel, M., Wright, K., Onopiuk, M., Tsiokas, L., & Humphrey, M. B. (2023). The inhibitor of MyoD Family A (I-MFA) regulates megakaryocyte lineage commitment and terminal differentiation. Blood Cells, Molecules, and Diseases, 102760.

Zhou, Z., Ma, X., Lin, Y., Cheng, D., Bavi, N., Secker, G. A., ... & Cox, C. D. (2023). MyoD-family inhibitor proteins act as auxiliary subunits of Piezo channels. Science, 381(6659), 799-804.

Lu, T., Zhu, Y., Guo, J., Mo, Z., Zhou, Q., Hu, C. Y., & Wang, C. (2023). MDFI regulates fast-to-slow muscle fiber type transformation via the calcium signaling pathway. Biochemical and Biophysical Research Communications.

Mi, C., Zhao, Y., Ren, L., & Zhang, D. (2022). Inhibition of MDFI attenuates proliferation and glycolysis of Helicobacter pylori‐infected gastric cancer cells by inhibiting Wnt/β‐catenin pathway. Cell Biology International, 46(12), 2198-2206.

Chen, P., Quan, Z., Song, X., Gao, Z., & Yuan, K. (2022). MDFI is a novel biomarker for poor prognosis in LUAD. Frontiers in Oncology, 12, 1005962.

Huang, B., Jiao, Y., Zhu, Y., Ning, Z., Ye, Z., Li, Q. X., ... & Wang, C. (2021). Mdfi promotes C2C12 cell differentiation and positively modulates fast-to-slow-twitch muscle fiber transformation. Frontiers in Cell and Developmental Biology, 9, 605875.

Huang, B., Jiao, Y., Zhu, Y., Ning, Z., Ye, Z., Li, Q. X., ... & Wang, C. (2021). Putative microrna-mrna networks upon mdfi overexpression in c2c12 cell differentiation and muscle fiber type transformation. Frontiers in Molecular Biosciences, 8, 675993.

Sui, Y., Li, X., Oh, S., Zhang, B., Freeman, W. M., Shin, S., & Janknecht, R. (2020). Opposite roles of the JMJD1A interaction partners MDFI and MDFIC in colorectal cancer. Scientific Reports, 10(1), 8710.

Hou, L., Zhu, L., Li, H., Jiang, F., Cao, L., Hu, C. Y., & Wang, C. (2019). MiR-501-3p forms a feedback loop with FOS, MDFI, and MyoD to regulate C2C12 myogenesis. Cells, 8(6), 573.

Ask a question We look forward to hearing from you.
0 reviews or Q&As

Purchasing FAQs
Technical FAQs

Loading...
Have you used Mouse Anti-MDFI Recombinant Antibody (3B4)?
Submit a review and get a Coupon or an Amazon gift card. 20% off Coupon $30 eGift Card
Submit a review
Loading...
For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

Online Inquiry

Documents

Contact us

  • Tel: (USA)
  • (UK)
  • Fax:
  • Email:

Submit A Review

Go to
Compare