Mouse Anti-MDFI (AA 33-110) Recombinant Antibody (CBFYM-1936) (CBMAB-M2110-FY)

Name: Mouse Anti-MDFI (AA 33-110) Recombinant Antibody (CBFYM-1936)
SKU: CBMAB-M2110-FY
Rating: 5 (1 reviews)

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Datasheet

SDS

Request for COA

Datasheet Target References Q & As Review & reward Protocols Associated Products

Basic Information

Host Animal

Mouse

Clone

CBFYM-1936

Application

IF, S-ELISA, ELISA, WB-Re, WB-Tr

Immunogen

Recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.

Specificity

Human

Antibody Isotype

IgG2a, k

Clonality

Monoclonal

Application Notes

The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Formulations & Storage [For reference only, actual COA shall prevail!]

Format

Liquid

Buffer

PBS, pH 7.2

Storage

Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

Epitope

AA 33-110

More Infomation

Target

Full Name

MyoD family inhibitor

Introduction

This protein is a transcription factor that negatively regulates other myogenic family proteins. Studies of the mouse homolog, I-mf, show that it interferes with myogenic factor function by masking nuclear localization signals and preventing DNA binding. Knockout mouse studies show defects in the formation of vertebrae and ribs that also involve cartilage formation in these structures.

Entrez Gene ID

4188

UniProt ID

Q99750

Alternative Names

MyoD Family Inhibitor; Inhibitor Of MyoD Family A; Myogenic Repressor I-Mf; I-Mfa; I-MF

Function

Inhibits the transactivation activity of the Myod family of myogenic factors and represses myogenesis. Acts by associating with Myod family members and retaining them in the cytoplasm by masking their nuclear localization signals. Can also interfere with the DNA-binding activity of Myod family members. Plays an important role in trophoblast and chondrogenic differentiation. Regulates the transcriptional activity of TCF7L1/TCF3 by interacting directly with TCF7L1/TCF3 and preventing it from binding DNA. Binds to the axin complex, resulting in an increase in the level of free beta-catenin. Affects axin regulation of the WNT and JNK signaling pathways (By similarity).

Biological Process

Cytoplasmic sequestering of transcription factor Source: ProtInc
Dorsal/ventral axis specification Source: UniProtKB
Embryonic skeletal system morphogenesis Source: Ensembl
Negative regulation of DNA binding Source: UniProtKB
Negative regulation of transcription by RNA polymerase II Source: UniProtKB
Negative regulation of Wnt signaling pathway Source: UniProtKB
Trophoblast giant cell differentiation Source: Ensembl

Cellular Location

Nucleus
Cytoplasm

Houser, J. S., Patel, M., Wright, K., Onopiuk, M., Tsiokas, L., & Humphrey, M. B. (2023). The inhibitor of MyoD Family A (I-MFA) regulates megakaryocyte lineage commitment and terminal differentiation. Blood Cells, Molecules, and Diseases, 102760.

Zhou, Z., Ma, X., Lin, Y., Cheng, D., Bavi, N., Secker, G. A., ... & Cox, C. D. (2023). MyoD-family inhibitor proteins act as auxiliary subunits of Piezo channels. Science, 381(6659), 799-804.

Lu, T., Zhu, Y., Guo, J., Mo, Z., Zhou, Q., Hu, C. Y., & Wang, C. (2023). MDFI regulates fast-to-slow muscle fiber type transformation via the calcium signaling pathway. Biochemical and Biophysical Research Communications.

Mi, C., Zhao, Y., Ren, L., & Zhang, D. (2022). Inhibition of MDFI attenuates proliferation and glycolysis of Helicobacter pylori‐infected gastric cancer cells by inhibiting Wnt/β‐catenin pathway. Cell Biology International, 46(12), 2198-2206.

Chen, P., Quan, Z., Song, X., Gao, Z., & Yuan, K. (2022). MDFI is a novel biomarker for poor prognosis in LUAD. Frontiers in Oncology, 12, 1005962.

Huang, B., Jiao, Y., Zhu, Y., Ning, Z., Ye, Z., Li, Q. X., ... & Wang, C. (2021). Mdfi promotes C2C12 cell differentiation and positively modulates fast-to-slow-twitch muscle fiber transformation. Frontiers in Cell and Developmental Biology, 9, 605875.

Huang, B., Jiao, Y., Zhu, Y., Ning, Z., Ye, Z., Li, Q. X., ... & Wang, C. (2021). Putative microrna-mrna networks upon mdfi overexpression in c2c12 cell differentiation and muscle fiber type transformation. Frontiers in Molecular Biosciences, 8, 675993.

Sui, Y., Li, X., Oh, S., Zhang, B., Freeman, W. M., Shin, S., & Janknecht, R. (2020). Opposite roles of the JMJD1A interaction partners MDFI and MDFIC in colorectal cancer. Scientific Reports, 10(1), 8710.

Hou, L., Zhu, L., Li, H., Jiang, F., Cao, L., Hu, C. Y., & Wang, C. (2019). MiR-501-3p forms a feedback loop with FOS, MDFI, and MyoD to regulate C2C12 myogenesis. Cells, 8(6), 573.

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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme-Linked Immunospot (ELISpot)
Proteogenomics
Other Protocols

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For research use only. Not intended for any clinical use.

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