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Mouse Anti-MDH2 Recombinant Antibody (2F5AF8) (CBMAB-M2118-FY)

This product is mouse antibody that recognizes MDH2. The antibody 2F5AF8 can be used for immunoassay techniques such as: ELISA, ICC, IP.
See all MDH2 antibodies

Summary

Host Animal
Mouse
Specificity
Human
Clone
2F5AF8
Antibody Isotype
IgG1, k
Application
ELISA, ICC, IP

Basic Information

Specificity
Human
Antibody Isotype
IgG1, k
Clonality
Monoclonal
Application Notes
The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Formulations & Storage [For reference only, actual COA shall prevail!]

Format
Liquid
Preservative
0.02% Sodium azide
Storage
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

Target

Full Name
MDH2
Introduction
Malate dehydrogenase catalyzes the reversible oxidation of malate to oxaloacetate, utilizing the NAD/NADH cofactor system in the citric acid cycle. The protein encoded by this gene is localized to the mitochondria and may play pivotal roles in the malate-aspartate shuttle that operates in the metabolic coordination between cytosol and mitochondria. Several transcript variants encoding different isoforms have been found for this gene.
Entrez Gene ID
4191
UniProt ID
P40926
Alternative Names
Malate Dehydrogenase 2; Malate Dehydrogenase 2, NAD (Mitochondrial); EC 1.1.1.37; Malate Dehydrogenase, Mitochondrial; Testicular Tissue Protein Li 120; MGC:3559
Biological Process
Aerobic respiration Source: UniProtKB
Malate metabolic process Source: UniProtKB
NADH metabolic process Source: Ensembl
Oxaloacetate metabolic process Source: Ensembl
Tricarboxylic acid cycle Source: GO_Central
Cellular Location
Mitochondrion matrix
Involvement in disease
Developmental and epileptic encephalopathy 51 (DEE51):
A form of epileptic encephalopathy, a heterogeneous group of severe early-onset epilepsies characterized by refractory seizures, neurodevelopmental impairment, and poor prognosis. Development is normal prior to seizure onset, after which cognitive and motor delays become apparent. DEE51 is an autosomal recessive form characterized by onset of intractable seizures and hypotonia in the first days or weeks of life, and severely delayed psychomotor development.
PTM
Acetylation is enhanced by up to 67% after treatment either with trichostin A (TSA) or with nicotinamide (NAM) with the appearance of tri- and tetraacetylations. Glucose also increases acetylation by about 60%.

Noble, M., Chatterjee, A., Sekaran, T., Schwarzl, T., & Hentze, M. W. (2023). Cytosolic RNA binding of the mitochondrial TCA cycle enzyme malate dehydrogenase (MDH2). bioRxiv, 2023-12.

Altea-Manzano, P., Vandekeere, A., Edwards-Hicks, J., Roldan, M., Abraham, E., Lleshi, X., ... & Finch, A. J. (2022). Reversal of mitochondrial malate dehydrogenase 2 enables anaplerosis via redox rescue in respiration-deficient cells. Molecular Cell, 82(23), 4537-4547.

Jungtrakoon Thamtarana, P., Marucci, A., Pannone, L., Bonnefond, A., Pezzilli, S., Biagini, T., ... & Prudente, S. (2022). Gain of function of malate dehydrogenase 2 and familial hyperglycemia. The Journal of Clinical Endocrinology & Metabolism, 107(3), 668-684.

Eleftheriadis, T., Pissas, G., Golfinopoulos, S., Efthymiadi, M., Liakopoulos, V., & Stefanidis, I. (2022). Inhibition of Malate Dehydrogenase-2 Protects Renal Tubular Epithelial Cells from Anoxia-Reoxygenation-Induced Death or Senescence. Biomolecules, 12(10), 1415.

Priestley, J. R., Pace, L. M., Sen, K., Aggarwal, A., Alves, C. A. P., Campbell, I. M., ... & Ganetzky, R. D. (2022). Malate dehydrogenase 2 deficiency is an emerging cause of pediatric epileptic encephalopathy with a recognizable biochemical signature. Molecular Genetics and Metabolism Reports, 33, 100931.

Molinié, T., Cougouilles, E., David, C., Cahoreau, E., Portais, J. C., & Mourier, A. (2022). MDH2 produced OAA is a metabolic switch rewiring the fuelling of respiratory chain and TCA cycle. Biochimica et Biophysica Acta (BBA)-Bioenergetics, 1863(3), 148532.

Ma, Y. C., Tian, P. F., Chen, Z. P., Yue, D. S., Liu, C. C., Li, C. G., ... & Wang, C. L. (2021). Urinary malate dehydrogenase 2 is a new biomarker for early detection of non‐small‐cell lung cancer. Cancer Science, 112(6), 2349-2360.

Ahmadi, F., Engel, M., & Baradarani, M. M. (2021). Synthesis, biological evaluation and molecular docking studies of indeno [1, 2-c] pyrazol derivatives as inhibitors of mitochondrial malate dehydrogenase 2 (MDH2). Bioorganic Chemistry, 110, 104779.

Xu, F., Hua, Q., Zhang, A., Di, Z., Wang, Y., Zhao, L., ... & Huang, G. (2021). LncRNA AC020978 facilitates non–small cell lung cancer progression by interacting with malate dehydrogenase 2 and activating the AKT pathway. Cancer Science, 112(11), 4501-4514.

Pezzilli, S., Jungtrakoon, P., Pannone, L., Marucci, A., Biagini, T., Buranasupkajorn, P., ... & Prudente, S. (2019). Gain of function mutations in Malate Dehydrogenase 2 (MDH2) cause familial diabetes of the adulthood. DIABETOLOGIA, 62, S145-S146.

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For research use only. Not intended for any clinical use.

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