Human MDH2 ELISA Kit (V2LY-0626-LY4851)

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Tested Data
Datasheet
SDS
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Datasheet Target References Q & As Review & reward Protocols Associated Products

Basic Information

Sensitivity
0.0076 ng/mL
Detection Range
0.015-3 ng/mL
Sample Type
Serum, Plasma, cell culture supernates
Specificity
Human
Assay Type
Sandwich
Reactivity
Human
Assay Time
1.5 h
Molecule Mass
35.5 kDa
Components
  • Pre-coated ELISA plate: 12 wells * 8 detachable strips
  • Standard solution: 0.5ml x1
  • Standard diluent: 3ml x1
  • Streptavidin-HRP: 6ml x1
  • Stop solution: 6ml x1
  • Substrate solution A: 6ml x1
  • Substrate solution B: 6ml x1
  • Wash buffer concentrate (25x): 20ml x1
  • Biotinylated antibody: 1ml x1

Formulations & Storage [For reference only, actual COA shall prevail!]

Storage
Store at 2-8°C
More Infomation

Target

Full Name
MDH2
Biological Process
Aerobic respiration Source: UniProtKB
Malate metabolic process Source: UniProtKB
NADH metabolic process Source: Ensembl
Oxaloacetate metabolic process Source: Ensembl
Tricarboxylic acid cycle Source: GO_Central
Cellular Location
Mitochondrion matrix
Involvement in disease
Developmental and epileptic encephalopathy 51 (DEE51):
A form of epileptic encephalopathy, a heterogeneous group of severe early-onset epilepsies characterized by refractory seizures, neurodevelopmental impairment, and poor prognosis. Development is normal prior to seizure onset, after which cognitive and motor delays become apparent. DEE51 is an autosomal recessive form characterized by onset of intractable seizures and hypotonia in the first days or weeks of life, and severely delayed psychomotor development.
PTM
Acetylation is enhanced by up to 67% after treatment either with trichostin A (TSA) or with nicotinamide (NAM) with the appearance of tri- and tetraacetylations. Glucose also increases acetylation by about 60%.

Noble, M., Chatterjee, A., Sekaran, T., Schwarzl, T., & Hentze, M. W. (2023). Cytosolic RNA binding of the mitochondrial TCA cycle enzyme malate dehydrogenase (MDH2). bioRxiv, 2023-12.

Altea-Manzano, P., Vandekeere, A., Edwards-Hicks, J., Roldan, M., Abraham, E., Lleshi, X., ... & Finch, A. J. (2022). Reversal of mitochondrial malate dehydrogenase 2 enables anaplerosis via redox rescue in respiration-deficient cells. Molecular Cell, 82(23), 4537-4547.

Jungtrakoon Thamtarana, P., Marucci, A., Pannone, L., Bonnefond, A., Pezzilli, S., Biagini, T., ... & Prudente, S. (2022). Gain of function of malate dehydrogenase 2 and familial hyperglycemia. The Journal of Clinical Endocrinology & Metabolism, 107(3), 668-684.

Eleftheriadis, T., Pissas, G., Golfinopoulos, S., Efthymiadi, M., Liakopoulos, V., & Stefanidis, I. (2022). Inhibition of Malate Dehydrogenase-2 Protects Renal Tubular Epithelial Cells from Anoxia-Reoxygenation-Induced Death or Senescence. Biomolecules, 12(10), 1415.

Priestley, J. R., Pace, L. M., Sen, K., Aggarwal, A., Alves, C. A. P., Campbell, I. M., ... & Ganetzky, R. D. (2022). Malate dehydrogenase 2 deficiency is an emerging cause of pediatric epileptic encephalopathy with a recognizable biochemical signature. Molecular Genetics and Metabolism Reports, 33, 100931.

Molinié, T., Cougouilles, E., David, C., Cahoreau, E., Portais, J. C., & Mourier, A. (2022). MDH2 produced OAA is a metabolic switch rewiring the fuelling of respiratory chain and TCA cycle. Biochimica et Biophysica Acta (BBA)-Bioenergetics, 1863(3), 148532.

Ma, Y. C., Tian, P. F., Chen, Z. P., Yue, D. S., Liu, C. C., Li, C. G., ... & Wang, C. L. (2021). Urinary malate dehydrogenase 2 is a new biomarker for early detection of non‐small‐cell lung cancer. Cancer Science, 112(6), 2349-2360.

Ahmadi, F., Engel, M., & Baradarani, M. M. (2021). Synthesis, biological evaluation and molecular docking studies of indeno [1, 2-c] pyrazol derivatives as inhibitors of mitochondrial malate dehydrogenase 2 (MDH2). Bioorganic Chemistry, 110, 104779.

Xu, F., Hua, Q., Zhang, A., Di, Z., Wang, Y., Zhao, L., ... & Huang, G. (2021). LncRNA AC020978 facilitates non–small cell lung cancer progression by interacting with malate dehydrogenase 2 and activating the AKT pathway. Cancer Science, 112(11), 4501-4514.

Pezzilli, S., Jungtrakoon, P., Pannone, L., Marucci, A., Biagini, T., Buranasupkajorn, P., ... & Prudente, S. (2019). Gain of function mutations in Malate Dehydrogenase 2 (MDH2) cause familial diabetes of the adulthood. DIABETOLOGIA, 62, S145-S146.

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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme Linked Immunospot (ELISpot)
Proteogenomic
Other Protocols

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For research use only. Not intended for any clinical use.

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