Mouse Anti-METTL1 Recombinant Antibody (CBWJC-4143) (CBMAB-C5487WJ)

Name: Mouse Anti-METTL1 Recombinant Antibody (CBWJC-4143)
SKU: CBMAB-C5487WJ
Rating: 5 (1 reviews)

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Datasheet

SDS

Request for COA

Datasheet Target References Q & As Review & reward Protocols Associated Products

Basic Information

Host Animal

Mouse

Clone

CBWJC-4143

Application

ELISA, WB

Immunogen

Recombinant Human METTL1 protein. This antibody was produced from a hybridoma resulting from the fusion of a mouse myeloma with B cells obtained from a mouse immunized with purified, recombinant Human METTL1

Specificity

Human

Antibody Isotype

IgG1

Clonality

Monoclonal

Application Notes

The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Formulations & Storage [For reference only, actual COA shall prevail!]

Storage

Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

More Infomation

Target

Full Name

Methyltransferase Like 1

Introduction

This gene is similar in sequence to the S. cerevisiae YDL201w gene. The gene product contains a conserved S-adenosylmethionine-binding motif and is inactivated by phosphorylation. Alternative splice variants encoding different protein isoforms have been described for this gene. A pseudogene has been identified on chromosome X. [provided by RefSeq, Jul 2008]

Entrez Gene ID

4234

UniProt ID

Q9UBP6

Alternative Names

Methyltransferase Like 1; TRNA (Guanine(46)-N(7))-Methyltransferase; Methyltransferase-Like Protein 1; TRNA(M7G46)-Methyltransferase; EC 2.1.1.33; C12orf1; TRNA (Guanine-N(7)-)-Methyltransferase;

Function

Methyltransferase that mediates the formation of N7-methylguanine in a subset of RNA species, such as tRNAs, mRNAs and microRNAs (miRNAs) (PubMed:12403464, PubMed:31031084, PubMed:31031083).

Catalyzes the formation of N7-methylguanine at position 46 (m7G46) in tRNA (PubMed:12403464, PubMed:31031084).

Also acts as a methyltransferase for a subset of internal N7-methylguanine in mRNAs (PubMed:31031084).

Internal N7-methylguanine methylation of mRNAs regulates translation (PubMed:31031084).

Also methylates a specific subset of miRNAs, such as let-7 (PubMed:31031083).

N7-methylguanine methylation of let-7 miRNA promotes let-7 miRNA processing by disrupting an inhibitory secondary structure within the primary miRNA transcript (pri-miRNA) (PubMed:31031083).

Acts as a regulator of embryonic stem cell self-renewal and differentiation (By similarity).

Biological Process

RNA (guanine-N7)-methylation Source: GO_Central
tRNA methylation Source: GO_Central
tRNA modification Source: UniProtKB

Cellular Location

Nucleus

PTM

Phosphorylation at Ser-27 inactivates its catalytic activity but does not affect the interaction with WDR4.

Ruiz-Arroyo, V. M., Raj, R., Babu, K., Onolbaatar, O., Roberts, P. H., & Nam, Y. (2023). Structures and mechanisms of tRNA methylation by METTL1–WDR4. Nature, 613(7943), 383-390.

Li, J., Wang, L., Hahn, Q., Nowak, R. P., Viennet, T., Orellana, E. A., ... & Gregory, R. I. (2023). Structural basis of regulated m7G tRNA modification by METTL1–WDR4. Nature, 613(7943), 391-397.

García-Vílchez, R., Añazco-Guenkova, A. M., Dietmann, S., López, J., Morón-Calvente, V., D’ambrosi, S., ... & Blanco, S. (2023). METTL1 promotes tumorigenesis through tRNA-derived fragment biogenesis in prostate cancer. Molecular Cancer, 22(1), 119.

Cheng, W., Gao, A., Lin, H., & Zhang, W. (2022). Novel roles of METTL1/WDR4 in tumor via m7G methylation. Molecular Therapy-Oncolytics.

Li, L., Yang, Y., Wang, Z., Xu, C., Huang, J., & Li, G. (2021). Prognostic role of METTL1 in glioma. Cancer cell international, 21(1), 1-20.

Orellana, E. A., Liu, Q., Yankova, E., Pirouz, M., De Braekeleer, E., Zhang, W., ... & Gregory, R. I. (2021). METTL1-mediated m7G modification of Arg-TCT tRNA drives oncogenic transformation. Molecular cell, 81(16), 3323-3338.

Ma, J., Han, H., Huang, Y., Yang, C., Zheng, S., Cai, T., ... & Lin, S. (2021). METTL1/WDR4-mediated m7G tRNA modifications and m7G codon usage promote mRNA translation and lung cancer progression. Molecular Therapy, 29(12), 3422-3435.

Chen, Z., Zhu, W., Zhu, S., Sun, K., Liao, J., Liu, H., ... & Lin, S. (2021). METTL1 promotes hepatocarcinogenesis via m7G tRNA modification‐dependent translation control. Clinical and translational medicine, 11(12), e661.

Pandolfini, L., Barbieri, I., Bannister, A. J., Hendrick, A., Andrews, B., Webster, N., ... & Kouzarides, T. (2019). METTL1 promotes let-7 MicroRNA processing via m7G methylation. Molecular cell, 74(6), 1278-1290.

Tian, Q. H., Zhang, M. F., Zeng, J. S., Luo, R. G., Wen, Y., Chen, J., ... & Xiong, J. P. (2019). METTL1 overexpression is correlated with poor prognosis and promotes hepatocellular carcinoma via PTEN. Journal of Molecular Medicine, 97, 1535-1545.

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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme Linked Immunospot (ELISpot)
Proteogenomic
Other Protocols

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For research use only. Not intended for any clinical use.

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