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Mouse Anti-METTL1 Recombinant Antibody (CBWJC-4143) (CBMAB-C5487WJ)

This product is a Mouse antibody that recognizes METTL1. This antibody CBWJC-4143 can be used for immunoassay techniques such as: ELISA, WB.
See all METTL1 antibodies

Summary

Host Animal
Mouse
Specificity
Human
Clone
CBWJC-4143
Antibody Isotype
IgG1
Application
ELISA, WB

Basic Information

Immunogen
Recombinant Human METTL1 protein. This antibody was produced from a hybridoma resulting from the fusion of a mouse myeloma with B cells obtained from a mouse immunized with purified, recombinant Human METTL1
Specificity
Human
Antibody Isotype
IgG1
Clonality
Monoclonal
Application Notes
The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Formulations & Storage [For reference only, actual COA shall prevail!]

Storage
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

Target

Full Name
Methyltransferase Like 1
Introduction
This gene is similar in sequence to the S. cerevisiae YDL201w gene. The gene product contains a conserved S-adenosylmethionine-binding motif and is inactivated by phosphorylation. Alternative splice variants encoding different protein isoforms have been described for this gene. A pseudogene has been identified on chromosome X. [provided by RefSeq, Jul 2008]
Entrez Gene ID
4234
UniProt ID
Q9UBP6
Alternative Names
Methyltransferase Like 1; TRNA (Guanine(46)-N(7))-Methyltransferase; Methyltransferase-Like Protein 1; TRNA(M7G46)-Methyltransferase; EC 2.1.1.33; C12orf1; TRNA (Guanine-N(7)-)-Methyltransferase;
Function
Methyltransferase that mediates the formation of N7-methylguanine in a subset of RNA species, such as tRNAs, mRNAs and microRNAs (miRNAs) (PubMed:12403464, PubMed:31031084, PubMed:31031083).

Catalyzes the formation of N7-methylguanine at position 46 (m7G46) in tRNA (PubMed:12403464, PubMed:31031084).

Also acts as a methyltransferase for a subset of internal N7-methylguanine in mRNAs (PubMed:31031084).

Internal N7-methylguanine methylation of mRNAs regulates translation (PubMed:31031084).

Also methylates a specific subset of miRNAs, such as let-7 (PubMed:31031083).

N7-methylguanine methylation of let-7 miRNA promotes let-7 miRNA processing by disrupting an inhibitory secondary structure within the primary miRNA transcript (pri-miRNA) (PubMed:31031083).

Acts as a regulator of embryonic stem cell self-renewal and differentiation (By similarity).
Biological Process
RNA (guanine-N7)-methylation Source: GO_Central
tRNA methylation Source: GO_Central
tRNA modification Source: UniProtKB
Cellular Location
Nucleus
PTM
Phosphorylation at Ser-27 inactivates its catalytic activity but does not affect the interaction with WDR4.

Ruiz-Arroyo, V. M., Raj, R., Babu, K., Onolbaatar, O., Roberts, P. H., & Nam, Y. (2023). Structures and mechanisms of tRNA methylation by METTL1–WDR4. Nature, 613(7943), 383-390.

Li, J., Wang, L., Hahn, Q., Nowak, R. P., Viennet, T., Orellana, E. A., ... & Gregory, R. I. (2023). Structural basis of regulated m7G tRNA modification by METTL1–WDR4. Nature, 613(7943), 391-397.

García-Vílchez, R., Añazco-Guenkova, A. M., Dietmann, S., López, J., Morón-Calvente, V., D’ambrosi, S., ... & Blanco, S. (2023). METTL1 promotes tumorigenesis through tRNA-derived fragment biogenesis in prostate cancer. Molecular Cancer, 22(1), 119.

Cheng, W., Gao, A., Lin, H., & Zhang, W. (2022). Novel roles of METTL1/WDR4 in tumor via m7G methylation. Molecular Therapy-Oncolytics.

Li, L., Yang, Y., Wang, Z., Xu, C., Huang, J., & Li, G. (2021). Prognostic role of METTL1 in glioma. Cancer cell international, 21(1), 1-20.

Orellana, E. A., Liu, Q., Yankova, E., Pirouz, M., De Braekeleer, E., Zhang, W., ... & Gregory, R. I. (2021). METTL1-mediated m7G modification of Arg-TCT tRNA drives oncogenic transformation. Molecular cell, 81(16), 3323-3338.

Ma, J., Han, H., Huang, Y., Yang, C., Zheng, S., Cai, T., ... & Lin, S. (2021). METTL1/WDR4-mediated m7G tRNA modifications and m7G codon usage promote mRNA translation and lung cancer progression. Molecular Therapy, 29(12), 3422-3435.

Chen, Z., Zhu, W., Zhu, S., Sun, K., Liao, J., Liu, H., ... & Lin, S. (2021). METTL1 promotes hepatocarcinogenesis via m7G tRNA modification‐dependent translation control. Clinical and translational medicine, 11(12), e661.

Pandolfini, L., Barbieri, I., Bannister, A. J., Hendrick, A., Andrews, B., Webster, N., ... & Kouzarides, T. (2019). METTL1 promotes let-7 MicroRNA processing via m7G methylation. Molecular cell, 74(6), 1278-1290.

Tian, Q. H., Zhang, M. F., Zeng, J. S., Luo, R. G., Wen, Y., Chen, J., ... & Xiong, J. P. (2019). METTL1 overexpression is correlated with poor prognosis and promotes hepatocellular carcinoma via PTEN. Journal of Molecular Medicine, 97, 1535-1545.

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For research use only. Not intended for any clinical use.

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