Human Recombinant METTL1 protein, GST Tag (V2LY-0526-LY5561)

Name: Human Recombinant METTL1 protein, GST Tag
SKU: V2LY-0526-LY5561
Rating: 5 (1 reviews)

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Datasheet

SDS

Request for COA

Datasheet Target References Q & As Review & reward Protocols Associated Products

Basic Information

Expressed Host

Baculovirus-Insect Cells

Protein Species

Human

Tag

GST Tag

Protein Construction

This product is Human Recombinant METTL1 protein, GST Tag consist of Amino Acid: Full Length and predicts a molecular mass of 57 kDa.

Molecule Mass

57 kDa

Sequence

Amino Acid: Full Length

Species

Human

Formulations & Storage [For reference only, actual COA shall prevail!]

Purity

Batch dependent.

Endotoxin

Please contact us for more information.

Format

Liquid

Buffer

MOPS, NaCl, Glutathione, DTT, PMSF, Glycerol

Preservative

None

Storage

Store product at -70°C. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles.

More Infomation

Target

Full Name

Methyltransferase Like 1

Function

Methyltransferase that mediates the formation of N7-methylguanine in a subset of RNA species, such as tRNAs, mRNAs and microRNAs (miRNAs) (PubMed:12403464, PubMed:31031084, PubMed:31031083).

Catalyzes the formation of N7-methylguanine at position 46 (m7G46) in tRNA (PubMed:12403464, PubMed:31031084).

Also acts as a methyltransferase for a subset of internal N7-methylguanine in mRNAs (PubMed:31031084).

Internal N7-methylguanine methylation of mRNAs regulates translation (PubMed:31031084).

Also methylates a specific subset of miRNAs, such as let-7 (PubMed:31031083).

N7-methylguanine methylation of let-7 miRNA promotes let-7 miRNA processing by disrupting an inhibitory secondary structure within the primary miRNA transcript (pri-miRNA) (PubMed:31031083).

Acts as a regulator of embryonic stem cell self-renewal and differentiation (By similarity).

Biological Process

RNA (guanine-N7)-methylation Source: GO_Central
tRNA methylation Source: GO_Central
tRNA modification Source: UniProtKB

Cellular Location

Nucleus

PTM

Phosphorylation at Ser-27 inactivates its catalytic activity but does not affect the interaction with WDR4.

Ruiz-Arroyo, V. M., Raj, R., Babu, K., Onolbaatar, O., Roberts, P. H., & Nam, Y. (2023). Structures and mechanisms of tRNA methylation by METTL1–WDR4. Nature, 613(7943), 383-390.

Li, J., Wang, L., Hahn, Q., Nowak, R. P., Viennet, T., Orellana, E. A., ... & Gregory, R. I. (2023). Structural basis of regulated m7G tRNA modification by METTL1–WDR4. Nature, 613(7943), 391-397.

García-Vílchez, R., Añazco-Guenkova, A. M., Dietmann, S., López, J., Morón-Calvente, V., D’ambrosi, S., ... & Blanco, S. (2023). METTL1 promotes tumorigenesis through tRNA-derived fragment biogenesis in prostate cancer. Molecular Cancer, 22(1), 119.

Cheng, W., Gao, A., Lin, H., & Zhang, W. (2022). Novel roles of METTL1/WDR4 in tumor via m7G methylation. Molecular Therapy-Oncolytics.

Li, L., Yang, Y., Wang, Z., Xu, C., Huang, J., & Li, G. (2021). Prognostic role of METTL1 in glioma. Cancer cell international, 21(1), 1-20.

Orellana, E. A., Liu, Q., Yankova, E., Pirouz, M., De Braekeleer, E., Zhang, W., ... & Gregory, R. I. (2021). METTL1-mediated m7G modification of Arg-TCT tRNA drives oncogenic transformation. Molecular cell, 81(16), 3323-3338.

Ma, J., Han, H., Huang, Y., Yang, C., Zheng, S., Cai, T., ... & Lin, S. (2021). METTL1/WDR4-mediated m7G tRNA modifications and m7G codon usage promote mRNA translation and lung cancer progression. Molecular Therapy, 29(12), 3422-3435.

Chen, Z., Zhu, W., Zhu, S., Sun, K., Liao, J., Liu, H., ... & Lin, S. (2021). METTL1 promotes hepatocarcinogenesis via m7G tRNA modification‐dependent translation control. Clinical and translational medicine, 11(12), e661.

Pandolfini, L., Barbieri, I., Bannister, A. J., Hendrick, A., Andrews, B., Webster, N., ... & Kouzarides, T. (2019). METTL1 promotes let-7 MicroRNA processing via m7G methylation. Molecular cell, 74(6), 1278-1290.

Tian, Q. H., Zhang, M. F., Zeng, J. S., Luo, R. G., Wen, Y., Chen, J., ... & Xiong, J. P. (2019). METTL1 overexpression is correlated with poor prognosis and promotes hepatocellular carcinoma via PTEN. Journal of Molecular Medicine, 97, 1535-1545.

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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme Linked Immunospot (ELISpot)
Proteogenomic
Other Protocols

Alternative Versions

Human Recombinant METTL1 protein, His Tag (CAT#: V2LY-0526-LY5562)

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Human Recombinant METTL1 protein, His Tag (CAT#: V2LY-0526-LY5562)

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Isotype control

For research use only. Not intended for any clinical use.

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