Mouse Anti-MGMT Recombinant Antibody (CBFYM-0373) (CBMAB-M0462-FY)

Name: Mouse Anti-MGMT Recombinant Antibody (CBFYM-0373)
SKU: CBMAB-M0462-FY
Rating: 5 (1 reviews)

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Datasheet

SDS

Request for COA

Datasheet Target References Q & As Review & reward Protocols Associated Products

Basic Information

Host Animal

Mouse

Clone

CBFYM-0373

Application

WB, FC, IHC, IF, IHC-P, FC, CyTOF

Immunogen

Recombinant human MGMT (O6-methylguanine-DNA methyltransferase) purified from E. coli

Specificity

Human

Antibody Isotype

IgG2b

Clonality

Monoclonal

Application Notes

The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Formulations & Storage [For reference only, actual COA shall prevail!]

Format

Liquid

Concentration

1 mg/mL

Storage

Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

More Infomation

Target

Full Name

O-6-Methylguanine-DNA Methyltransferase

Introduction

Alkylating agents are potent carcinogens that can result in cell death, mutation and cancer. The protein encoded by this gene is a DNA repair protein that is involved in cellular defense against mutagenesis and toxicity from alkylating agents. The protein catalyzes transfer of methyl groups from O-alkylguanine and other methylated moieties of the DNA to its own molecule, which repairs the toxic lesions. Methylation of the genes promoter has been associated with several cancer types, including colorectal cancer, lung cancer, lymphoma and glioblastoma.

Entrez Gene ID

4255

UniProt ID

P16455

Alternative Names

O-6-Methylguanine-DNA Methyltransferase; Methylated-DNA--Protein-Cysteine Methyltransferase; -O-Methylguanine-DNA Methyltransferase; O-6-Methylguanine-DNA-Alkyltransferase; EC 2.1.1.63; O6-Methylguanine-DNA Methyltransferase; Methylguanine-DNA Methyltransferase

Function

Involved in the cellular defense against the biological effects of O6-methylguanine (O6-MeG) and O4-methylthymine (O4-MeT) in DNA. Repairs the methylated nucleobase in DNA by stoichiometrically transferring the methyl group to a cysteine residue in the enzyme. This is a suicide reaction: the enzyme is irreversibly inactivated.

Biological Process

Cellular response to ionizing radiation Source: Ensembl
Cellular response to organic cyclic compound Source: Ensembl
Cellular response to oxidative stress Source: Ensembl
DNA dealkylation involved in DNA repair Source: Ensembl
DNA ligation Source: ProtInc
DNA repair Source: GO_Central
Mammary gland epithelial cell differentiation Source: Ensembl
Negative regulation of apoptotic process Source: BHF-UCL
Positive regulation of double-strand break repair Source: BHF-UCL
Regulation of cysteine-type endopeptidase activity involved in apoptotic process Source: Ensembl
Response to ethanol Source: Ensembl
Response to folic acid Source: Ensembl
Response to toxic substance Source: Ensembl
Response to xenobiotic stimulus Source: Ensembl

Cellular Location

Nucleus

Chen, Y., Qu, W., Tu, J., & Qi, H. (2023). Implications of Advances in Studies of O6-Methylguanine-DNA-Methyltransferase for Tumor Prognosis and Treatment. Frontiers in Bioscience-Landmark, 28(9), 197.

Chen, X., Sun, J., Li, Y., Jiang, W., Li, Z., Mao, J., ... & Tan, G. (2023). Proteomic and metabolomic analyses illustrate the mechanisms of expression of the O6‐methylguanine‐DNA methyltransferase gene in glioblastoma. CNS Neuroscience & Therapeutics.

Kostka, T., Empl, M. T., Seiwert, N., Geisen, S. M., Hoffmann, P., Adam, J., ... & Steinberg, P. (2021). Repair of O 6-carboxymethylguanine adducts by O 6-methylguanine-DNA methyltransferase in human colon epithelial cells. Carcinogenesis, 42(8), 1110-1118.

McAleenan, A., Kelly, C., Spiga, F., Kernohan, A., Cheng, H. Y., Dawson, S., ... & Kurian, K. M. (2021). Prognostic value of test (s) for O6‐methylguanine–DNA methyltransferase (MGMT) promoter methylation for predicting overall survival in people with glioblastoma treated with temozolomide. Cochrane Database of Systematic Reviews, (3).

Yu, W., Zhang, L., Wei, Q., & Shao, A. (2020). O6-methylguanine-DNA methyltransferase (MGMT): challenges and new opportunities in glioma chemotherapy. Frontiers in oncology, 9, 1547.

Dovek, L., Nguyen, N. T., Ozer, B. H., Li, N., Elashoff, R. M., Green, R. M., ... & Lai, A. (2019). Correlation of commercially available quantitative MGMT (O-6-methylguanine-DNA methyltransferase) promoter methylation scores and GBM patient survival. Neuro-Oncology Practice, 6(3), 194-202.

Feldheim, J., Kessler, A. F., Monoranu, C. M., Ernestus, R. I., Löhr, M., & Hagemann, C. (2019). Changes of O6-methylguanine DNA methyltransferase (MGMT) promoter methylation in glioblastoma relapse—a meta-analysis type literature review. Cancers, 11(12), 1837.

Yang, L., Li, W., Zhao, Y., Zhong, S., Wang, X., Jiang, S., ... & Zhao, G. (2019). Computational study of novel natural inhibitors targeting O6-methylguanine-DNA methyltransferase. World neurosurgery, 130, e294-e306.

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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme-Linked Immunospot (ELISpot)
Proteogenomics
Other Protocols

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Isotype control

For research use only. Not intended for any clinical use.

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We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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