Rabbit Anti-MMP16 (AA 121-545) Recombinant Antibody (CBFYM-2375) (CBMAB-M2562-FY)

Name: Rabbit Anti-MMP16 (AA 121-545) Recombinant Antibody (CBFYM-2375)
SKU: CBMAB-M2562-FY
Rating: 5 (1 reviews)

See all MMP16 antibodies

Compare Online Inquiry

Datasheet

SDS

Request for COA

Datasheet Target References Q & As Review & reward Protocols Associated Products

Basic Information

Host Animal

Rabbit

Clone

CBFYM-2375

Application

FC, ICC, IF, IHC-P, WB

Immunogen

Recombinant protein corresponding to amino acids 121-545 of human MMP-16

Specificity

Human, Rat

Antibody Isotype

IgG

Clonality

Monoclonal

Application Notes

The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Formulations & Storage [For reference only, actual COA shall prevail!]

Format

Liquid

Buffer

PBS

Preservative

0.09% Sodium azide

Concentration

0.5 mg/mL

Storage

Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

Epitope

AA 121-545

More Infomation

Target

Full Name

MATRIX METALLOPEPTIDASE 16

Introduction

Proteins of the matrix metalloproteinase family are involved in the breakdown of extracellular matrix in normal physiological processes, such as embryonic development, reproduction, and tissue remodeling, as well as in disease processes, such as arthritis and metastasis. Most MMP's are secreted as inactive proproteins which are activated when cleaved by extracellular proteinases. The encoded protein activates MMP2 by cleavage. This gene was once referred to as MT-MMP2, but was renamed as MT-MMP3 or MMP16.

Entrez Gene ID

Human	4325
Rat	65205

UniProt ID

Human	P51512
Rat	O35548

Alternative Names

Matrix Metallopeptidase 16; Matrix Metallopeptidase 16 (Membrane-Inserted); Membrane-Type Matrix Metalloproteinase 3; Membrane-Type-3 Matrix Metalloproteinase; MT-MMP 3; C8orf57; MT3-MMP; MMP-16; MMP-X2; MT3MMP; MTMMP3

Function

Endopeptidase that degrades various components of the extracellular matrix, such as collagen type III and fibronectin. Activates progelatinase A. Involved in the matrix remodeling of blood vessels. Isoform short cleaves fibronectin and also collagen type III, but at lower rate. It has no effect on type I, II, IV and V collagen. However, upon interaction with CSPG4, it may be involved in degradation and invasion of type I collagen by melanoma cells.

Biological Process

Chondrocyte proliferation Source: Ensembl
Collagen catabolic process Source: GO_Central
Craniofacial suture morphogenesis Source: Ensembl
Embryonic cranial skeleton morphogenesis Source: Ensembl
Endochondral ossification Source: Ensembl
Extracellular matrix organization Source: GO_Central
Protein processing Source: ParkinsonsUK-UCL
Proteolysis Source: ParkinsonsUK-UCL
Skeletal system development Source: GO_Central

Cellular Location

Isoform Long:
Plasma membrane
Cell membrane
Note: Localized at the cell surface of melanoma cells.
Isoform Short:
Extracellular region or secreted
extracellular matrix
Other locations
Cell surface
Note: Localized at the cell surface of melanoma cells.

Topology

Extracellular: 120-564
Helical: 565-585
Cytoplasmic: 586-607

PTM

The precursor is cleaved by a furin endopeptidase.

Pietrzak, J., Szmajda-Krygier, D., Wosiak, A., Świechowski, R., Michalska, K., Mirowski, M., ... & Balcerczak, E. (2022). Changes in the expression of membrane type-matrix metalloproteinases genes (MMP14, MMP15, MMP16, MMP24) during treatment and their potential impact on the survival of patients with non-small cell lung cancer (NSCLC). Biomedicine & Pharmacotherapy, 146, 112559.

Wang, H., Qi, C., & Wan, D. (2021). MicroRNA-377-3p targeting MMP-16 inhibits ovarian cancer cell growth, invasion, and interstitial transition. Annals of Translational Medicine, 9(2).

Zhang, Y., Zeng, S., & Wang, T. (2021). Circular RNA hsa_circ_0002360 promotes non-small cell lung cancer progression through upregulating matrix metalloproteinase 16 and sponging multiple micorRNAs. Bioengineered, 12(2), 12767-12777.

Li, W., Song, Z., Jia, N., Zhang, C., Gao, W., & Wang, L. (2021). microRNA-4429-5p suppresses the malignant development of colon cancer by targeting matrix metalloproteinase 16. In Vitro Cellular & Developmental Biology-Animal, 57(7), 715-725.

Lou, L., Tian, M., Chang, J., Li, F., & Zhang, G. (2020). MiRNA-192-5p attenuates airway remodeling and autophagy in asthma by targeting MMP-16 and ATG7. Biomedicine & Pharmacotherapy, 122, 109692.

Ask a question We look forward to hearing from you.

0 reviews or Q&As

Purchasing FAQs
Technical FAQs

Have you used Rabbit Anti-MMP16 (AA 121-545) Recombinant Antibody (CBFYM-2375)?
Submit a review and get a Coupon or an Amazon gift card. 20% off Coupon

$30 eGift Card
Submit a review

Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme-Linked Immunospot (ELISpot)
Proteogenomics
Other Protocols

Related Products

Mouse Anti-MMP16 (AA 32-535) Recombinant Antibody (CBFYM-0206) (CAT#: CBMAB-M0248-FY)

Mouse Anti-MMP16 Recombinant Antibody (CBFYM-0673) (CAT#: CBMAB-M0809-FY)

Mouse Anti-MMP16 (AA 168-181) Recombinant Antibody (CBFYM-2374) (CAT#: CBMAB-M2561-FY)

Mouse Anti-MMP16 Recombinant Antibody (CBFYM-2373) (CAT#: CBMAB-M2560-FY)

Isotype control

For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

Online Inquiry

Request bulk or custom quote Second antibody and isotype control

Contact us

Tel: (USA)
(UK)
Fax:

Global Locations

Email:

Online Inquiry