Recombinant Mouse Anti-MNAT1 Recombinant Antibody (1G6) (CBMAB-XB0829-YC)

Provided herein is a Mouse Recombinant Antibody against MNAT1, CDK Activating Kinase Assembly Factor. The antibody can be used for immunoassay techniques, such as ELISA, IF, WB.
See all MNAT1 antibodies

Datasheet

Summary

Host Animal

Mouse

Specificity

Human

Clone

1G6

Antibody Isotype

IgG1, κ

Application

ELISA, IF, WB

Basic Information

Immunogen

MDDQGCPRCKTTKYRNPSL

Specificity

Human

Antibody Isotype

IgG1, κ

Clonality

Monoclonal

Application Notes

The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Formulations & Storage [For reference only, actual COA shall prevail!]

Storage

Store at 4°C short term (1-2 weeks). Aliquot and store at-20°C long term. Avoid repeated freeze/thaw cycles.

Target

Full Name

MNAT1, CDK Activating Kinase Assembly Factor

Introduction

MNAT1, along with cyclin H and CDK7, forms the CDK-activating kinase (CAK) enzymatic complex. This complex activates several cyclin-associated kinases and can also associate with TFIIH to activate transcription by RNA polymerase II.

Entrez Gene ID

4331

UniProt ID

P51948

Alternative Names

MNAT1, CDK Activating Kinase Assembly Factor; MNAT CDK-Activating Kinase Assembly Factor 1; Menage A Trois 1 (CAK Assembly Factor); CDK7/Cyclin-H Assembly Factor; RING Finger Protein MAT1; RING Finger Protein 66; CAP35; RNF66; MAT1; Menage A Trois Homolog 1, Cyclin H Assembly Factor (Xenopus Laevis); Menage A Trois-Like Protein 1 Cyclin H Assembly Factor;

Function

Stabilizes the cyclin H-CDK7 complex to form a functional CDK-activating kinase (CAK) enzymatic complex. CAK activates the cyclin-associated kinases CDK1, CDK2, CDK4 and CDK6 by threonine phosphorylation. CAK complexed to the core-TFIIH basal transcription factor activates RNA polymerase II by serine phosphorylation of the repetitive C-terminal domain (CTD) of its large subunit (POLR2A), allowing its escape from the promoter and elongation of the transcripts. Involved in cell cycle control and in RNA transcription by RNA polymerase II.

Biological Process

Adult heart development Source: Ensembl
DNA repair Source: GO_Central
G1/S transition of mitotic cell cycle Source: Ensembl
Negative regulation of apoptotic process Source: Ensembl
Negative regulation of DNA helicase activity Source: UniProtKB
Nucleotide-excision repair Source: InterPro
Positive regulation of smooth muscle cell proliferation Source: Ensembl
Protein-containing complex assembly Source: ProtInc
Regulation of cyclin-dependent protein serine/threonine kinase activity Source: GO_Central
Regulation of G1/S transition of mitotic cell cycle Source: ComplexPortal
Regulation of transcription by RNA polymerase II Source: UniProtKB
Response to calcium ion Source: Ensembl
Transcription by RNA polymerase II Source: UniProtKB
Ventricular system development Source: Ensembl

Cellular Location

Nucleus

References

Xu, Z., Liu, Y., Pan, Z., & Qin, L. (2023). Epigenetic upregulation of MNAT1 by SMYD2 is linked to PI3K/AKT activation and tumorigenesis of pancreatic adenocarcinoma. Histology and Histopathology, 18630-18630.

Zhou, S., Lu, J., Li, Y., Chen, C., Cai, Y., Tan, G., ... & Tang, F. (2021). Retraction Note: MNAT1 is overexpressed in colorectal cancer and mediates p53 ubiquitin-degradation to promote colorectal cancer malignance. Journal of Experimental & Clinical Cancer Research: CR, 40.

Zou, C., Mifflin, L., Hu, Z., Zhang, T., Shan, B., Wang, H., ... & Yuan, J. (2020). Reduction of mNAT1/hNAT2 contributes to cerebral endothelial necroptosis and Aβ accumulation in Alzheimer’s disease. Cell reports, 33(10).

Qiu, C., Su, W., Shen, N., Qi, X., Wu, X., Wang, K., ... & Xiang, H. (2020). MNAT1 promotes proliferation and the chemo-resistance of osteosarcoma cell to cisplatin through regulating PI3K/Akt/mTOR pathway. BMC cancer, 20(1), 1-12.

Wang, J., Ni, J., Song, D., Ding, M., Huang, J., Li, W., & He, G. (2020). The regulatory effect of has-circ-0001146/miR-26a-5p/MNAT1 network on the proliferation and invasion of osteosarcoma. Bioscience Reports, 40(6), BSR20201232.

Chen, Y., Guan, J. X., & Shen, H. (2019). MNAT1 expression in non-small cell lung cancer and its biological cellular impact. Zhonghua Bing li xue za zhi= Chinese Journal of Pathology, 48(8), 626-632.

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Protocols

Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme Linked Immunospot (ELISpot)
Proteogenomic
Other Protocols

Associated Products

Related Products

Mouse Anti-MNAT1 Recombinant Antibody (CBFYM-2435)

Isotype control

For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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