• Home
  • MOCS3
  • Mouse Anti-MOCS3 Recombinant Antibody (1C5-E8)

Mouse Anti-MOCS3 Recombinant Antibody (1C5-E8) (CBMAB-A5545-LY)

See all MOCS3 antibodies
Go to compare Compare Online Inquiry
Datasheet
SDS
Request for COA
Datasheet Target References Q & As Review & reward Protocols Associated Products

Basic Information

Host Animal
Mouse
Clone
1C5-E8
Application
WB, ELISA
Immunogen
MOCS3 (AAH15939, 1 a.a. ~ 460 a.a) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
Specificity
Human
Antibody Isotype
IgG2b, κ
Clonality
Monoclonal
Application Notes
The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Formulations & Storage [For reference only, actual COA shall prevail!]

Format
Liquid
Purity
> 95% Purity determined by SDS-PAGE.
Storage
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freezethaw cycles.
More Infomation

Target

Full Name
Molybdenum Cofactor Synthesis 3
Introduction
Molybdenum cofactor (MoCo) is necessary for the function of all molybdoenzymes. One of the enzymes required for the biosynthesis of MoCo is molybdopterin synthase (MPT synthase). The protein encoded by this gene adenylates and activates MPT synthase. This gene contains no introns. A pseudogene of this gene is present on chromosome 14. [provided by RefSeq]
Entrez Gene ID
27304
UniProt ID
O95396
Alternative Names
MGC9252; UBA4; dJ914P20.3
Function
Plays a central role in 2-thiolation of mcm5S2U at tRNA wobble positions of cytosolic tRNA(Lys), tRNA(Glu) and tRNA(Gln). Also essential during biosynthesis of the molybdenum cofactor. Acts by mediating the C-terminal thiocarboxylation of sulfur carriers URM1 and MOCS2A. Its N-terminus first activates URM1 and MOCS2A as acyl-adenylates (-COAMP), then the persulfide sulfur on the catalytic cysteine is transferred to URM1 and MOCS2A to form thiocarboxylation (-COSH) of their C-terminus. The reaction probably involves hydrogen sulfide that is generated from the persulfide intermediate and that acts as nucleophile towards URM1 and MOCS2A. Subsequently, a transient disulfide bond is formed. Does not use thiosulfate as sulfur donor; NFS1 probably acting as a sulfur donor for thiocarboxylation reactions.
Biological Process
Mo-molybdopterin cofactor biosynthetic process Source: UniProtKB
Protein urmylation Source: GO_Central
tRNA thio-modification Source: UniProtKB
tRNA wobble position uridine thiolation Source: GO_Central
tRNA wobble uridine modification Source: UniProtKB
Cellular Location
Cytoplasm

Tian, Q., Cao, Y., Shu, L., Chen, Y., Peng, Y., Wang, Y., ... & Mao, X. (2021). Case Report: Compound Heterozygous Variants in MOCS3 Identified in a Chinese Infant With Molybdenum Cofactor Deficiency. Frontiers in Genetics, 12, 651878.

Neukranz, Y., Kotter, A., Beilschmidt, L., Marelja, Z., Helm, M., Gräf, R., & Leimkühler, S. (2019). Analysis of the Cellular Roles of MOCS3 Identifies a MOCS3-Independent Localization of NFS1 at the Tips of the Centrosome. Biochemistry, 58(13), 1786-1798.

Neukranz, Y. (2019). MOCS3 and its role in molybdenum cofactor biosynthesis, tRNA thiolation and other cellular pathways in humans (Doctoral dissertation, Universität Potsdam).

Ask a question We look forward to hearing from you.
0 reviews or Q&As

Purchasing FAQs
Technical FAQs

Loading...
Have you used Mouse Anti-MOCS3 Recombinant Antibody (1C5-E8)?
Submit a review and get a Coupon or an Amazon gift card. 20% off Coupon $30 eGift Card
Submit a review
Loading...

Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme Linked Immunospot (ELISpot)
Proteogenomic
Other Protocols

Antibody Pairs

MOCS3 Matched Antibody Pair (744) (CAT#: APMAB-744LY)

MOCS3 Matched Antibody Pair (745) (CAT#: APMAB-745LY)

Related Products

Mouse Anti-MOCS3 (AA 1-461) Recombinant Antibody (CBFYM-0487) (CAT#: CBMAB-M0615-FY)

MOCS3 Matched Antibody Pair (745) (CAT#: APMAB-745LY)

Mouse Anti-MOCS3 (AA 1-461) Recombinant Antibody (CBFYM-2462) (CAT#: CBMAB-M2649-FY)

MOCS3 Matched Antibody Pair (744) (CAT#: APMAB-744LY)

Isotype control

For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

Online Inquiry
Request bulk or custom quote Second antibody and isotype control

Contact us

  • Tel: (USA)
  • (UK)
  • Fax:
  • Email:

Submit A Review

online inquiry
Online Inquiry