Mouse Anti-MOCS3 (AA 1-461) Recombinant Antibody (CBFYM-0487) (CBMAB-M0615-FY)

Name: Mouse Anti-MOCS3 (AA 1-461) Recombinant Antibody (CBFYM-0487)
SKU: CBMAB-M0615-FY
Rating: 5 (1 reviews)

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Datasheet

SDS

Request for COA

Datasheet Target References Q & As Review & reward Protocols Associated Products

Basic Information

Host Animal

Mouse

Clone

CBFYM-0487

Application

ELISA, WB

Specificity

Human

Antibody Isotype

IgG2b

Clonality

Monoclonal

Application Notes

The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Formulations & Storage [For reference only, actual COA shall prevail!]

Format

Liquid

Buffer

PBS, pH 7.2

Preservative

0.09% Sodium azide

Storage

Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

Epitope

AA 1-461

More Infomation

Target

Full Name

Molybdenum Cofactor Synthesis 3

Introduction

Molybdenum cofactor is necessary for the function of all molybdoenzymes. The protein encoded by this gene adenylates and activates molybdopterin synthase, an enzyme required for biosynthesis of MoCo. This gene contains no introns. A pseudogene of this gene is present on chromosome 14.

Entrez Gene ID

27304

UniProt ID

O95396

Alternative Names

Molybdenum Cofactor Synthesis 3; Ubiquitin-Like Modifier Activating Enzyme 4; Molybdenum Cofactor Synthesis Protein 3; Molybdopterin Synthase Sulfurylase; MPT Synthase Sulfurylase; UBA4; UBA4, Ubiquitin-Activating Enzyme E1 Homolog (Yeast); Adenylyltransferase And Sulfurtransferase MOCS3; UBA4, Ubiquitin-Activating Enzyme E1 Homolog

Function

Plays a central role in 2-thiolation of mcm5S2U at tRNA wobble positions of cytosolic tRNA(Lys), tRNA(Glu) and tRNA(Gln). Also essential during biosynthesis of the molybdenum cofactor. Acts by mediating the C-terminal thiocarboxylation of sulfur carriers URM1 and MOCS2A. Its N-terminus first activates URM1 and MOCS2A as acyl-adenylates (-COAMP), then the persulfide sulfur on the catalytic cysteine is transferred to URM1 and MOCS2A to form thiocarboxylation (-COSH) of their C-terminus. The reaction probably involves hydrogen sulfide that is generated from the persulfide intermediate and that acts as nucleophile towards URM1 and MOCS2A. Subsequently, a transient disulfide bond is formed. Does not use thiosulfate as sulfur donor; NFS1 probably acting as a sulfur donor for thiocarboxylation reactions.

Biological Process

Mo-molybdopterin cofactor biosynthetic process Source: UniProtKB
Protein urmylation Source: GO_Central
tRNA thio-modification Source: UniProtKB
tRNA wobble position uridine thiolation Source: GO_Central
tRNA wobble uridine modification Source: UniProtKB

Cellular Location

Cytoplasm

Tian, Q., Cao, Y., Shu, L., Chen, Y., Peng, Y., Wang, Y., ... & Mao, X. (2021). Case Report: Compound Heterozygous Variants in MOCS3 Identified in a Chinese Infant With Molybdenum Cofactor Deficiency. Frontiers in Genetics, 12, 651878.

Neukranz, Y., Kotter, A., Beilschmidt, L., Marelja, Z., Helm, M., Gräf, R., & Leimkühler, S. (2019). Analysis of the Cellular Roles of MOCS3 Identifies a MOCS3-Independent Localization of NFS1 at the Tips of the Centrosome. Biochemistry, 58(13), 1786-1798.

Neukranz, Y. (2019). MOCS3 and its role in molybdenum cofactor biosynthesis, tRNA thiolation and other cellular pathways in humans (Doctoral dissertation, Universität Potsdam).

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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme Linked Immunospot (ELISpot)
Proteogenomic
Other Protocols

Antibody Pairs

MOCS3 Matched Antibody Pair (744) (CAT#: APMAB-744LY)

MOCS3 Matched Antibody Pair (745) (CAT#: APMAB-745LY)

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MOCS3 Matched Antibody Pair (744) (CAT#: APMAB-744LY)

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MOCS3 Matched Antibody Pair (745) (CAT#: APMAB-745LY)

Mouse Anti-MOCS3 Recombinant Antibody (1C5-E8) (CAT#: CBMAB-A5545-LY)

Isotype control

For research use only. Not intended for any clinical use.

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We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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