Mouse Anti-MPL Recombinant Antibody (CBT3823) (V2LY-0625-LY778)

Name: Mouse Anti-MPL Recombinant Antibody (CBT3823)
SKU: V2LY-0625-LY778
Rating: 5 (1 reviews)

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Tested Data

Mouse Anti-MPL Recombinant Antibody (CBT3823) (V2LY-0625-LY778) in ELISA

Mouse Anti-MPL Recombinant Antibody (CBT3823) (V2LY-0625-LY778) in WB

Mouse Anti-MPL Recombinant Antibody (CBT3823) (V2LY-0625-LY778) in FC

Datasheet

SDS

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Datasheet Target References Q & As Review & reward Protocols Associated Products

Basic Information

Host Animal

Mouse

Clone

CBT3823

Application

Immunogen

Purified recombinant fragment of human CD110 (AA: extra 26-175) expressed in E. Coli.

Host Species

Mouse

Specificity

Human

Antibody Isotype

IgG2a

Clonality

Monoclonal Antibody

Application Notes

Application	Note
WB	1:500-1:2,000
FC	1:200-1:400
ELISA	1:10,000

Formulations & Storage [For reference only, actual COA shall prevail!]

Format

Liquid

Buffer

PBS

Preservative

Sodium azide

Concentration

Batch dependent

Purity

> 95% Purity determined by SDS-PAGE.

Storage

Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freezethaw cycles.

More Infomation

Target

Full Name

MPL Proto-Oncogene, Thrombopoietin Receptor

Entrez Gene ID

4352

UniProt ID

P40238

Function

Receptor for thrombopoietin that acts as a primary regulator of megakaryopoiesis and platelet production. May represent a regulatory molecule specific for TPO-R-dependent immune responses.

Biological Process

Basophil homeostasis Source: Ensembl
Cellular response to hypoxia Source: Ensembl
Cytokine-mediated signaling pathway Source: GO_Central
Eosinophil homeostasis Source: Ensembl
Hemopoiesis Source: GO_Central
Monocyte homeostasis Source: Ensembl
Neutrophil homeostasis Source: Ensembl
Platelet aggregation Source: Ensembl
Positive regulation of cell population proliferation Source: GO_Central
Positive regulation of lymphocyte proliferation Source: Ensembl
Positive regulation of platelet formation Source: Ensembl
Thrombopoietin-mediated signaling pathway Source: GO_Central

Cellular Location

Plasma membrane
Cell membrane
Golgi apparatus
Other locations
Cell surface

Involvement in disease

Congenital amegakaryocytic thrombocytopenia (CAMT):
Disease characterized by isolated thrombocytopenia and megakaryocytopenia with no physical anomalies.
Thrombocythemia 2 (THCYT2):
A myeloproliferative disorder characterized by excessive platelet production, resulting in increased numbers of circulating platelets. It can be associated with spontaneous hemorrhages and thrombotic episodes.
Myelofibrosis with myeloid metaplasia (MMM):
A chronic myeloproliferative disorder characterized by replacement of the bone marrow by fibrous tissue, extramedullary hematopoiesis, anemia, leukoerythroblastosis and hepatosplenomegaly.

Topology

Extracellular: 26-491
Helical: 492-513
Cytoplasmic: 514-635

PTM

Ubiquitination at Lys-553 and Lys-573 targets MPL for degradation by both the lysosomal and proteasomal pathways. The E3 ubiquitin-protein ligase CBL significantly contributes to this ubiquitination.

Al-Nakhle, H. H., Yagoub, H. S., Anbarkhan, S. H., Alamri, G. A., & Alsubaie, N. M. (2023). In Silico Evaluation of Coding and Non-Coding nsSNPs in the Thrombopoietin Receptor (MPL) Proto-Oncogene: Assessing Their Influence on Protein Stability, Structure, and Function. Current Issues in Molecular Biology, 45(12), 9390-9412.

Zoine, J. T., Prince, C., Story, J. Y., Branella, G. M., Lytle, A. M., Fedanov, A., ... & Chandrakasan, S. (2022). Thrombopoietin-based CAR-T cells demonstrate in vitro and in vivo cytotoxicity to MPL positive acute myelogenous leukemia and hematopoietic stem cells. Gene Therapy, 29(5), 1-12.

Spivak, J. L., & Moliterno, A. R. (2021). The thrombopoietin receptor, MPL, is a therapeutic target of opportunity in the MPN. Frontiers in Oncology, 11, 641613.

Guglielmelli, P., & Calabresi, L. (2021). The MPL mutation. International Review of Cell and Molecular Biology, 365, 163-178.

Cui, L., Moraga, I., Lerbs, T., Van Neste, C., Wilmes, S., Tsutsumi, N., ... & Wernig, G. (2021). Tuning MPL signaling to influence hematopoietic stem cell differentiation and inhibit essential thrombocythemia progenitors. Proceedings of the National Academy of Sciences, 118(2), e2017849118.

Elbager, S. G., Makkawi, A. A., Mohamed, H. A., Abdelrahman, F. A., Osman, L. H., Hameed, M. F., ... & Bayoumi, M. (2019). In silico analysis of Missense Variants in Human MPL Gene Associated with Essential Thrombocythemia. bioRxiv, 830372.

Kohlscheen, S., Schenk, F., Rommel, M. G., Cullmann, K., & Modlich, U. (2019). Endothelial protein C receptor supports hematopoietic stem cell engraftment and expansion in Mpl-deficient mice. Blood, The Journal of the American Society of Hematology, 133(13), 1465-1478.

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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme Linked Immunospot (ELISpot)
Proteogenomic
Other Protocols

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Isotype control

For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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