Mouse Recombinant MPL protein, His Tag (V2LY-0526-LY9006)

Name: Mouse Recombinant MPL protein, His Tag
SKU: V2LY-0526-LY9006
Rating: 5 (1 reviews)

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Datasheet

SDS

Request for COA

Datasheet Target References Q & As Review & reward Protocols Associated Products

Basic Information

Expressed Host

HEK293 Cells

Protein Species

Mouse

Tag

His Tag

Protein Construction

This product is Mouse Recombinant MPL protein, His Tag consist of Amino Acid: 1-482 and predicts a molecular mass of 52.64 kDa.

Molecule Mass

52.64 kDa

Verified

HPLC

Sequence

Amino Acid: 1-482

Species

Mouse

Formulations & Storage [For reference only, actual COA shall prevail!]

Purity

≥95% as determined by SDS-PAGE. ≥95% as determined by SEC-HPLC.

Endotoxin

Please contact us for more information.

Format

Lyophilized

Reconstitution

Allow the vial and reconstitution buffer to equilibrate to room temperature. Briefly centrifuge or tap down the vial to ensure that all lyophilized powder is collected at the bottom of the vial. For the reconstitution of this product, we recommend adding PBS or sterile water to achieve a final antibody concentration of 1 mg/mL. Allow the vial to reconstitute for 10-15 minutes at room temperature with gentle agitation. Avoid vigorous shaking that can cause foaming and antibody denaturation. Aliquot into volumes based on your experiment and store liquid protein at -20°C or -80°C for long time.

Buffer

Lyophilized from sterile PBS

Preservative

None

Storage

Samples are stable for up to twelve months from date of receipt at -20°C to -80°C. Store it under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.

More Infomation

Target

Full Name

MPL Proto-Oncogene, Thrombopoietin Receptor

Function

Receptor for thrombopoietin that acts as a primary regulator of megakaryopoiesis and platelet production. May represent a regulatory molecule specific for TPO-R-dependent immune responses.

Biological Process

Basophil homeostasis Source: Ensembl
Cellular response to hypoxia Source: Ensembl
Cytokine-mediated signaling pathway Source: GO_Central
Eosinophil homeostasis Source: Ensembl
Hemopoiesis Source: GO_Central
Monocyte homeostasis Source: Ensembl
Neutrophil homeostasis Source: Ensembl
Platelet aggregation Source: Ensembl
Positive regulation of cell population proliferation Source: GO_Central
Positive regulation of lymphocyte proliferation Source: Ensembl
Positive regulation of platelet formation Source: Ensembl
Thrombopoietin-mediated signaling pathway Source: GO_Central

Cellular Location

Plasma membrane
Cell membrane
Golgi apparatus
Other locations
Cell surface

Involvement in disease

Congenital amegakaryocytic thrombocytopenia (CAMT):
Disease characterized by isolated thrombocytopenia and megakaryocytopenia with no physical anomalies.
Thrombocythemia 2 (THCYT2):
A myeloproliferative disorder characterized by excessive platelet production, resulting in increased numbers of circulating platelets. It can be associated with spontaneous hemorrhages and thrombotic episodes.
Myelofibrosis with myeloid metaplasia (MMM):
A chronic myeloproliferative disorder characterized by replacement of the bone marrow by fibrous tissue, extramedullary hematopoiesis, anemia, leukoerythroblastosis and hepatosplenomegaly.

Topology

Extracellular: 26-491
Helical: 492-513
Cytoplasmic: 514-635

PTM

Ubiquitination at Lys-553 and Lys-573 targets MPL for degradation by both the lysosomal and proteasomal pathways. The E3 ubiquitin-protein ligase CBL significantly contributes to this ubiquitination.

Al-Nakhle, H. H., Yagoub, H. S., Anbarkhan, S. H., Alamri, G. A., & Alsubaie, N. M. (2023). In Silico Evaluation of Coding and Non-Coding nsSNPs in the Thrombopoietin Receptor (MPL) Proto-Oncogene: Assessing Their Influence on Protein Stability, Structure, and Function. Current Issues in Molecular Biology, 45(12), 9390-9412.

Zoine, J. T., Prince, C., Story, J. Y., Branella, G. M., Lytle, A. M., Fedanov, A., ... & Chandrakasan, S. (2022). Thrombopoietin-based CAR-T cells demonstrate in vitro and in vivo cytotoxicity to MPL positive acute myelogenous leukemia and hematopoietic stem cells. Gene Therapy, 29(5), 1-12.

Spivak, J. L., & Moliterno, A. R. (2021). The thrombopoietin receptor, MPL, is a therapeutic target of opportunity in the MPN. Frontiers in Oncology, 11, 641613.

Guglielmelli, P., & Calabresi, L. (2021). The MPL mutation. International Review of Cell and Molecular Biology, 365, 163-178.

Cui, L., Moraga, I., Lerbs, T., Van Neste, C., Wilmes, S., Tsutsumi, N., ... & Wernig, G. (2021). Tuning MPL signaling to influence hematopoietic stem cell differentiation and inhibit essential thrombocythemia progenitors. Proceedings of the National Academy of Sciences, 118(2), e2017849118.

Elbager, S. G., Makkawi, A. A., Mohamed, H. A., Abdelrahman, F. A., Osman, L. H., Hameed, M. F., ... & Bayoumi, M. (2019). In silico analysis of Missense Variants in Human MPL Gene Associated with Essential Thrombocythemia. bioRxiv, 830372.

Kohlscheen, S., Schenk, F., Rommel, M. G., Cullmann, K., & Modlich, U. (2019). Endothelial protein C receptor supports hematopoietic stem cell engraftment and expansion in Mpl-deficient mice. Blood, The Journal of the American Society of Hematology, 133(13), 1465-1478.

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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme Linked Immunospot (ELISpot)
Proteogenomic
Other Protocols

Alternative Versions

Human Recombinant MPL protein, His Tag (CAT#: V2LY-0526-LY7340)

Rat Recombinant MPL protein, ECD, hFc Tag (CAT#: V2LY-0526-LY9618)

Rat Recombinant MPL protein, His Tag (CAT#: V2LY-0526-LY9619)

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Human Recombinant MPL protein, His Tag (CAT#: V2LY-0526-LY7340)

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Isotype control

For research use only. Not intended for any clinical use.

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We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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