Sign in or Register   Sign in or Register
  |  

Human Anti-MPL Recombinant Antibody (CBXC-0771) (CBMAB-C1885-CQ)

This product is a recombinant human antibody that recognizes MPL. The antibody CBXC-0771 can be used for immunoassay techniques such as: FC.
See all MPL antibodies

Summary

Host Animal
Human
Specificity
Human
Clone
CBXC-0771
Antibody Isotype
IgG1
Application
FC

Basic Information

Specificity
Human
Antibody Isotype
IgG1
Clonality
Monoclonal
Application Notes
The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Formulations & Storage [For reference only, actual COA shall prevail!]

Format
Liquid
Buffer
Buffer containing stabilizer
Preservative
0.05% sodium azide
Storage
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

Target

Full Name
MPL Proto-Oncogene, Thrombopoietin Receptor
Introduction
In 1990 an oncogene, v-mpl, was identified from the murine myeloproliferative leukemia virus that was capable of immortalizing bone marrow hematopoietic cells from different lineages. In 1992 the human homologue, named, c-mpl, was cloned. Sequence data revealed that c-mpl encoded a protein that was homologous with members of the hematopoietic receptor superfamily. Presence of anti-sense oligodeoxynucleotides of c-mpl inhibited megakaryocyte colony formation. The ligand for c-mpl, thrombopoietin, was cloned in 1994. Thrombopoietin was shown to be the major regulator of megakaryocytopoiesis and platelet formation. The protein encoded by the c-mpl gene, CD110, is a 635 amino acid transmembrane domain, with two extracellular cytokine receptor domains and two intracellular cytokine receptor box motifs. TPO-R deficient mice were severely thrombocytopenic, emphasizing the important role of CD110 and thrombopoietin in megakaryocyte and platelet formation. Upon binding of thrombopoietin CD110 is dimerized and the JAK family of non-receptor tyrosine kinases, as well as the STAT family, the MAPK family, the adaptor protein Shc and the receptors themselves become tyrosine phosphorylated.
Entrez Gene ID
4352
UniProt ID
P40238
Alternative Names
MPL Proto-Oncogene, Thrombopoietin Receptor; Myeloproliferative Leukemia Virus Oncogene; Myeloproliferative Leukemia Protein; Proto-Oncogene C-Mpl; TPO-R; TPOR; Thrombopoietin Receptor;
Function
Receptor for thrombopoietin that acts as a primary regulator of megakaryopoiesis and platelet production. May represent a regulatory molecule specific for TPO-R-dependent immune responses.
Biological Process
Basophil homeostasis Source: Ensembl
Cellular response to hypoxia Source: Ensembl
Cytokine-mediated signaling pathway Source: GO_Central
Eosinophil homeostasis Source: Ensembl
Hemopoiesis Source: GO_Central
Monocyte homeostasis Source: Ensembl
Neutrophil homeostasis Source: Ensembl
Platelet aggregation Source: Ensembl
Positive regulation of cell population proliferation Source: GO_Central
Positive regulation of lymphocyte proliferation Source: Ensembl
Positive regulation of platelet formation Source: Ensembl
Thrombopoietin-mediated signaling pathway Source: GO_Central
Cellular Location
Plasma membrane
Cell membrane
Golgi apparatus
Other locations
Cell surface
Involvement in disease
Congenital amegakaryocytic thrombocytopenia (CAMT):
Disease characterized by isolated thrombocytopenia and megakaryocytopenia with no physical anomalies.
Thrombocythemia 2 (THCYT2):
A myeloproliferative disorder characterized by excessive platelet production, resulting in increased numbers of circulating platelets. It can be associated with spontaneous hemorrhages and thrombotic episodes.
Myelofibrosis with myeloid metaplasia (MMM):
A chronic myeloproliferative disorder characterized by replacement of the bone marrow by fibrous tissue, extramedullary hematopoiesis, anemia, leukoerythroblastosis and hepatosplenomegaly.
Topology
Extracellular: 26-491
Helical: 492-513
Cytoplasmic: 514-635
PTM
Ubiquitination at Lys-553 and Lys-573 targets MPL for degradation by both the lysosomal and proteasomal pathways. The E3 ubiquitin-protein ligase CBL significantly contributes to this ubiquitination.

Al-Nakhle, H. H., Yagoub, H. S., Anbarkhan, S. H., Alamri, G. A., & Alsubaie, N. M. (2023). In Silico Evaluation of Coding and Non-Coding nsSNPs in the Thrombopoietin Receptor (MPL) Proto-Oncogene: Assessing Their Influence on Protein Stability, Structure, and Function. Current Issues in Molecular Biology, 45(12), 9390-9412.

Zoine, J. T., Prince, C., Story, J. Y., Branella, G. M., Lytle, A. M., Fedanov, A., ... & Chandrakasan, S. (2022). Thrombopoietin-based CAR-T cells demonstrate in vitro and in vivo cytotoxicity to MPL positive acute myelogenous leukemia and hematopoietic stem cells. Gene Therapy, 29(5), 1-12.

Spivak, J. L., & Moliterno, A. R. (2021). The thrombopoietin receptor, MPL, is a therapeutic target of opportunity in the MPN. Frontiers in Oncology, 11, 641613.

Guglielmelli, P., & Calabresi, L. (2021). The MPL mutation. International Review of Cell and Molecular Biology, 365, 163-178.

Cui, L., Moraga, I., Lerbs, T., Van Neste, C., Wilmes, S., Tsutsumi, N., ... & Wernig, G. (2021). Tuning MPL signaling to influence hematopoietic stem cell differentiation and inhibit essential thrombocythemia progenitors. Proceedings of the National Academy of Sciences, 118(2), e2017849118.

Elbager, S. G., Makkawi, A. A., Mohamed, H. A., Abdelrahman, F. A., Osman, L. H., Hameed, M. F., ... & Bayoumi, M. (2019). In silico analysis of Missense Variants in Human MPL Gene Associated with Essential Thrombocythemia. bioRxiv, 830372.

Kohlscheen, S., Schenk, F., Rommel, M. G., Cullmann, K., & Modlich, U. (2019). Endothelial protein C receptor supports hematopoietic stem cell engraftment and expansion in Mpl-deficient mice. Blood, The Journal of the American Society of Hematology, 133(13), 1465-1478.

Ask a question We look forward to hearing from you.
0 reviews or Q&As

Purchasing FAQs
Technical FAQs

Loading...
Have you used Human Anti-MPL Recombinant Antibody (CBXC-0771)?
Submit a review and get a Coupon or an Amazon gift card. 20% off Coupon $30 eGift Card
Submit a review
Loading...
For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

Online Inquiry

Documents

Contact us

  • Tel: (USA)
  • (UK)
  • Fax:
  • Email:

Submit A Review

Go to
Compare