Mouse Anti-MPP1 (AA 1-466) Recombinant Antibody (CBFYM-0791) (CBMAB-M0935-FY)

Name: Mouse Anti-MPP1 (AA 1-466) Recombinant Antibody (CBFYM-0791)
SKU: CBMAB-M0935-FY
Rating: 5 (1 reviews)

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Datasheet

SDS

Request for COA

Datasheet Target References Q & As Review & reward Protocols Associated Products

Basic Information

Host Animal

Mouse

Clone

CBFYM-0791

Application

ELISA, siRNA, WB

Immunogen

Full length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.Immunogen sequence: MTLKASEGES GGSMHTALSD LYLEHLLQKR SRPEAVSHPL NTVTEDMYTN GSPAPGSPAQ VKGQEVRKVR LIQFEKVTEE PMGITLKLNE KQSCTVARIL HGGMIHRQGS LHVGDEILEI NGTNVTNHSV DQLQKAMKET K

Specificity

Human

Antibody Isotype

IgG1, k

Clonality

Monoclonal

Application Notes

The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Formulations & Storage [For reference only, actual COA shall prevail!]

Format

Liquid

Storage

Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

Epitope

AA 1-466

More Infomation

Target

Full Name

Membrane Palmitoylated Protein 1

Introduction

This gene encodes the prototype of the membrane-associated guanylate kinase family proteins. MAGUKs interact with the cytoskeleton and regulate cell proliferation, signaling pathways, and intercellular junctions. The encoded protein is an extensively palmitoylated membrane phosphoprotein containing a PDZ domain, a Src homology 3 motif, and a guanylate kinase domain. This gene product interacts with various cytoskeletal proteins and cell junctional proteins in different tissue and cell types, and may be involved in the regulation of cell shape, hair cell development, neural patterning of the retina, and apico-basal polarity and tumor suppression pathways in non-erythroid cells. Multiple transcript variants encoding different isoforms have been found for this gene.

Entrez Gene ID

4354

UniProt ID

Q00013

Alternative Names

Membrane Palmitoylated Protein 1; Membrane Protein, Palmitoylated 1, 55kDa; Membrane Protein, Palmitoylated 1; DXS552E; EMP55; P55; Palmitoylated Erythrocyte Membrane Protein; Membrane Protein, Palmitoylated 1 (55kD); 55 KDa Erythrocyte Membrane Protein

Function

Essential regulator of neutrophil polarity. Regulates neutrophil polarization by regulating AKT1 phosphorylation through a mechanism that is independent of PIK3CG activity (By similarity).

Biological Process

Regulation of neutrophil chemotaxis Source: UniProtKB
Signal transduction Source: ProtInc

Cellular Location

Plasma membrane
Cell membrane
Other locations
stereocilium
Note: Colocalizes with WHRN at stereocilium tip during hair cell development (By similarity). Colocalizes with PALS1 in the retina, at the outer limiting membrane (OLM) (By similarity). Colocalizes with WHRN in the retina, at the outer limiting membrane (OLM), outer plexifirm layer (OPL), basal bodies and at the connecting cilium (CC) (By similarity). Colocalizes with NF2 in non-myelin-forming Schwann cells (PubMed:19144871).

PTM

Palmitoylated.

Biernatowska, A., Wójtowicz, K., Trombik, T., Sikorski, A. F., & Czogalla, A. (2022). MPP1 Determines the Mobility of Flotillins and Controls the Confinement of Raft-Associated Molecules. Cells, 11(3), 311.

Biernatowska, A., Olszewska, P., Grzymajło, K., Drabik, D., Kraszewski, S., Sikorski, A. F., & Czogalla, A. (2021). Molecular characterization of direct interactions between MPP1 and flotillins. Scientific Reports, 11(1), 14751.

Chytła, A., Gajdzik-Nowak, W., Biernatowska, A., Sikorski, A. F., & Czogalla, A. (2021). High-level expression of palmitoylated MPP1 recombinant protein in mammalian cells. Membranes, 11(9), 715.

Trybus, M., Niemiec, L., Biernatowska, A., Hryniewicz-Jankowska, A., & Sikorski, A. F. (2019). MPP1-based mechanism of resting state raft organization in the plasma membrane. Is it a general or specialized mechanism in erythroid cells?. Folia Histochemica et Cytobiologica, 57(2), 43-55.

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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme Linked Immunospot (ELISpot)
Proteogenomic
Other Protocols

Antibody Pairs

MPP1 Matched Antibody Pair (746) (CAT#: APMAB-746LY)

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MPP1 Matched Antibody Pair (746) (CAT#: APMAB-746LY)

Mouse Anti-MPP1 Recombinant Antibody (2E5) (CAT#: CBMAB-A5564-LY)

Isotype control

For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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