Human MPP1 ELISA Kit (V2LY-0626-LY5585)

Name: Human MPP1 ELISA Kit
SKU: V2LY-0626-LY5585
Rating: 5 (1 reviews)

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Tested Data

Datasheet

SDS

Request for COA

Datasheet Target References Q & As Review & reward Protocols Associated Products

Basic Information

Sensitivity

0.0074 ng/mL

Detection Range

0.015-3 ng/mL

Sample Type

Serum, Plasma, cell culture supernates

Specificity

Human

Assay Type

Sandwich

Reactivity

Human

Assay Time

1.5 h

Molecule Mass

52.3 kDa

Components

Pre-coated ELISA plate: 12 wells * 8 detachable strips
Standard solution: 0.5ml x1
Standard diluent: 3ml x1
Streptavidin-HRP: 6ml x1
Stop solution: 6ml x1
Substrate solution A: 6ml x1
Substrate solution B: 6ml x1
Wash buffer concentrate (25x): 20ml x1
Biotinylated antibody: 1ml x1

Formulations & Storage [For reference only, actual COA shall prevail!]

Storage

Store at 2-8°C

More Infomation

Target

Full Name

Membrane Palmitoylated Protein 1

Function

Essential regulator of neutrophil polarity. Regulates neutrophil polarization by regulating AKT1 phosphorylation through a mechanism that is independent of PIK3CG activity (By similarity).

Biological Process

Regulation of neutrophil chemotaxis Source: UniProtKB
Signal transduction Source: ProtInc

Cellular Location

Plasma membrane
Cell membrane
Other locations
stereocilium
Note: Colocalizes with WHRN at stereocilium tip during hair cell development (By similarity). Colocalizes with PALS1 in the retina, at the outer limiting membrane (OLM) (By similarity). Colocalizes with WHRN in the retina, at the outer limiting membrane (OLM), outer plexifirm layer (OPL), basal bodies and at the connecting cilium (CC) (By similarity). Colocalizes with NF2 in non-myelin-forming Schwann cells (PubMed:19144871).

PTM

Palmitoylated.

Biernatowska, A., Wójtowicz, K., Trombik, T., Sikorski, A. F., & Czogalla, A. (2022). MPP1 Determines the Mobility of Flotillins and Controls the Confinement of Raft-Associated Molecules. Cells, 11(3), 311.

Biernatowska, A., Olszewska, P., Grzymajło, K., Drabik, D., Kraszewski, S., Sikorski, A. F., & Czogalla, A. (2021). Molecular characterization of direct interactions between MPP1 and flotillins. Scientific Reports, 11(1), 14751.

Chytła, A., Gajdzik-Nowak, W., Biernatowska, A., Sikorski, A. F., & Czogalla, A. (2021). High-level expression of palmitoylated MPP1 recombinant protein in mammalian cells. Membranes, 11(9), 715.

Trybus, M., Niemiec, L., Biernatowska, A., Hryniewicz-Jankowska, A., & Sikorski, A. F. (2019). MPP1-based mechanism of resting state raft organization in the plasma membrane. Is it a general or specialized mechanism in erythroid cells?. Folia Histochemica et Cytobiologica, 57(2), 43-55.

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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme Linked Immunospot (ELISpot)
Proteogenomic
Other Protocols

Alternative Versions

MPP1 Matched Antibody Pair (746) (CAT#: APMAB-746LY)

Antibody Pairs

MPP1 Matched Antibody Pair (746) (CAT#: APMAB-746LY)

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MPP1 Matched Antibody Pair (746) (CAT#: APMAB-746LY)

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Isotype control

For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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