Mouse Anti-MSI1 Recombinant Antibody (CBT2667) (V2LY-0625-LY2554)

Name: Mouse Anti-MSI1 Recombinant Antibody (CBT2667)
SKU: V2LY-0625-LY2554
Rating: 5 (1 reviews)

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Tested Data

Mouse Anti-MSI1 Recombinant Antibody (CBT2667) (V2LY-0625-LY2554) in WB

Mouse Anti-MSI1 Recombinant Antibody (CBT2667) (V2LY-0625-LY2554) in FC

Mouse Anti-MSI1 Recombinant Antibody (CBT2667) (V2LY-0625-LY2554) in ELISA

Datasheet

SDS

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Datasheet Target References Q & As Review & reward Protocols Associated Products

Basic Information

Host Animal

Mouse

Clone

CBT2667

Application

WB, FC

Immunogen

Purified recombinant fragment of human MSI1 expressed in E. Coli.

Host Species

Mouse

Specificity

Human

Antibody Isotype

IgG1

Clonality

Monoclonal Antibody

Application Notes

Application	Note
WB	1:500-1:2,000
FC	1:200-1:400
ELISA	1:10,000

Formulations & Storage [For reference only, actual COA shall prevail!]

Format

Supernatant

Preservative

Sodium azide

Concentration

Batch dependent

Storage

Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freezethaw cycles.

More Infomation

Target

Full Name

Musashi RNA Binding Protein 1

Entrez Gene ID

4440

UniProt ID

O43347

Function

RNA binding protein that regulates the expression of target mRNAs at the translation level. Regulates expression of the NOTCH1 antagonist NUMB. Binds RNA containing the sequence 5'-GUUAGUUAGUUAGUU-3' and other sequences containing the pattern 5'-[GA]U1-3AGU-3'. May play a role in the proliferation and maintenance of stem cells in the central nervous system (By similarity).

Biological Process

Epithelial cell differentiation Source: Ensembl
Nervous system development Source: ProtInc
Response to hormone Source: Ensembl

Cellular Location

Cytoplasm
Nucleus

Darai, N., Mahalapbutr, P., Wolschann, P., Lee, V. S., Wolfinger, M. T., & Rungrotmongkol, T. (2022). Theoretical studies on RNA recognition by Musashi 1 RNA-binding protein. Scientific Reports, 12(1), 12137.

Löblein, M. T., Falke, I., Eich, H. T., Greve, B., Götte, M., & Troschel, F. M. (2021). Dual knockdown of musashi RNA-binding proteins MSI-1 and MSI-2 attenuates putative cancer stem cell characteristics and therapy resistance in ovarian cancer cells. International Journal of Molecular Sciences, 22(21), 11502.

Chen, H., Liu, J., Wang, H., Cheng, Q., Zhou, C., Chen, X., & Ye, F. (2019). Inhibition of RNA-binding protein musashi-1 suppresses malignant properties and reverses paclitaxel resistance in ovarian carcinoma. Journal of Cancer, 10(6), 1580.

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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme Linked Immunospot (ELISpot)
Proteogenomic
Other Protocols

Antibody Pairs

MSI1 Matched Antibody Pair (757) (CAT#: APMAB-757LY)

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Isotype control

For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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