Mouse Msi1 ELISA Kit (V2LY-0626-LY2305)

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Tested Data
Datasheet
SDS
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Datasheet Target References Q & As Review & reward Protocols Associated Products

Basic Information

Sensitivity
0.0052 ng/mL
Detection Range
0.01-2 ng/mL
Sample Type
Serum, Plasma, cell culture supernates
Specificity
Mouse
Assay Type
Sandwich
Reactivity
Mouse
Assay Time
1.5 h
Molecule Mass
39.1 kDa
Components
  • Pre-coated ELISA Plate: 12 wells * 8 detachable strips
  • Standard solution: 0.5ml x1
  • Standard diluent: 3ml x1
  • Streptavidin-HRP: 6ml x1
  • Stop solution: 6ml x1
  • Substrate solution A: 6ml x1
  • Substrate solution B: 6ml x1
  • Wash buffer concentrate (25x): 20ml x1
  • Biotinylated antibody: 1ml x1

Formulations & Storage [For reference only, actual COA shall prevail!]

Storage
Store at 2-8°C
More Infomation

Target

Full Name
Musashi RNA Binding Protein 1
Function
RNA binding protein that regulates the expression of target mRNAs at the translation level. Regulates expression of the NOTCH1 antagonist NUMB. Binds RNA containing the sequence 5'-GUUAGUUAGUUAGUU-3' and other sequences containing the pattern 5'-[GA]U1-3AGU-3'. May play a role in the proliferation and maintenance of stem cells in the central nervous system (By similarity).
Biological Process
Epithelial cell differentiation Source: Ensembl
Nervous system development Source: ProtInc
Response to hormone Source: Ensembl
Cellular Location
Cytoplasm
Nucleus

Darai, N., Mahalapbutr, P., Wolschann, P., Lee, V. S., Wolfinger, M. T., & Rungrotmongkol, T. (2022). Theoretical studies on RNA recognition by Musashi 1 RNA-binding protein. Scientific Reports, 12(1), 12137.

Löblein, M. T., Falke, I., Eich, H. T., Greve, B., Götte, M., & Troschel, F. M. (2021). Dual knockdown of musashi RNA-binding proteins MSI-1 and MSI-2 attenuates putative cancer stem cell characteristics and therapy resistance in ovarian cancer cells. International Journal of Molecular Sciences, 22(21), 11502.

Chen, H., Liu, J., Wang, H., Cheng, Q., Zhou, C., Chen, X., & Ye, F. (2019). Inhibition of RNA-binding protein musashi-1 suppresses malignant properties and reverses paclitaxel resistance in ovarian carcinoma. Journal of Cancer, 10(6), 1580.

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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme Linked Immunospot (ELISpot)
Proteogenomic
Other Protocols

Alternative Versions

Human MSI1 ELISA Kit (CAT#: V2LY-0626-LY5596)

MSI1 Matched Antibody Pair (757) (CAT#: APMAB-757LY)

Antibody Pairs

MSI1 Matched Antibody Pair (757) (CAT#: APMAB-757LY)

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Human MSI1 ELISA Kit (CAT#: V2LY-0626-LY5596)

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Isotype control

For research use only. Not intended for any clinical use.

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