Mouse Anti-MSI1 Recombinant Antibody (CBT898) (V2LY-0625-LY3188)

Name: Mouse Anti-MSI1 Recombinant Antibody (CBT898)
SKU: V2LY-0625-LY3188
Rating: 5 (1 reviews)

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Tested Data

Mouse Anti-MSI1 Recombinant Antibody (CBT898) (V2LY-0625-LY3188) in FC

Mouse Anti-MSI1 Recombinant Antibody (CBT898) (V2LY-0625-LY3188) in WB

Datasheet

Summary

Host Animal

Mouse

Specificity

Human

Clone

CBT898

Antibody Isotype

IgG

Application

WB, FC, ELISA

Basic Information

Immunogen

Purified recombinant fragment of human Msi1 expressed in E. Coli.

Host Species

Mouse

Specificity

Human

Antibody Isotype

IgG

Clonality

Monoclonal Antibody

Application Notes

Application	Note
ELISA	1:10,000
FC	1:200-1:400
WB	1:500-1:2,000

Formulations & Storage [For reference only, actual COA shall prevail!]

Format

Supernatant

Buffer

BSA, Glycerol

Preservative

Sodium azide

Concentration

Batch dependent

Storage

Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freezethaw cycles.

Target

Entrez Gene ID

4440

UniProt ID

O43347

Function

RNA binding protein that regulates the expression of target mRNAs at the translation level. Regulates expression of the NOTCH1 antagonist NUMB. Binds RNA containing the sequence 5'-GUUAGUUAGUUAGUU-3' and other sequences containing the pattern 5'-[GA]U1-3AGU-3'. May play a role in the proliferation and maintenance of stem cells in the central nervous system (By similarity).

Biological Process

Epithelial cell differentiation Source: Ensembl
Nervous system development Source: ProtInc
Response to hormone Source: Ensembl

Cellular Location

Cytoplasm
Nucleus

More Infomation

References

Darai, N., Mahalapbutr, P., Wolschann, P., Lee, V. S., Wolfinger, M. T., & Rungrotmongkol, T. (2022). Theoretical studies on RNA recognition by Musashi 1 RNA-binding protein. Scientific Reports, 12(1), 12137.

Löblein, M. T., Falke, I., Eich, H. T., Greve, B., Götte, M., & Troschel, F. M. (2021). Dual knockdown of musashi RNA-binding proteins MSI-1 and MSI-2 attenuates putative cancer stem cell characteristics and therapy resistance in ovarian cancer cells. International Journal of Molecular Sciences, 22(21), 11502.

Chen, H., Liu, J., Wang, H., Cheng, Q., Zhou, C., Chen, X., & Ye, F. (2019). Inhibition of RNA-binding protein musashi-1 suppresses malignant properties and reverses paclitaxel resistance in ovarian carcinoma. Journal of Cancer, 10(6), 1580.

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Protocols

Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme-Linked Immunospot (ELISpot)
Proteogenomics
Other Protocols

Associated Products

Antibody Pairs

MSI1 Matched Antibody Pair (757) (CAT#: APMAB-757LY)

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Isotype control

For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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Datasheet
SDS
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Second antibody and isotype control

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