Mouse Anti-MSLN Recombinant Antibody (CBFYM-0134) (CBMAB-M0154-FY)

Name: Mouse Anti-MSLN Recombinant Antibody (CBFYM-0134)
SKU: CBMAB-M0154-FY
Rating: 5 (1 reviews)

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Datasheet

SDS

Request for COA

Datasheet Target References Q & As Review & reward Protocols Associated Products

Basic Information

Host Animal

Mouse

Clone

CBFYM-0134

Application

ELISA, WB

Immunogen

Recombinant protein

Specificity

Human

Antibody Isotype

IgG2a

Application Notes

The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Formulations & Storage [For reference only, actual COA shall prevail!]

Format

Liquid

Storage

Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

More Infomation

Target

Full Name

Mesothelin

Introduction

This gene encodes a preproprotein that is proteolytically processed to generate two protein products, megakaryocyte potentiating factor and mesothelin. Megakaryocyte potentiating factor functions as a cytokine that can stimulate colony formation of bone marrow megakaryocytes. Mesothelin is a glycosylphosphatidylinositol-anchored cell-surface protein that may function as a cell adhesion protein. This protein is overexpressed in epithelial mesotheliomas, ovarian cancers and in specific squamous cell carcinomas. Alternative splicing results in multiple transcript variants, at least one of which encodes an isoform that is proteolytically processed.

Entrez Gene ID

10232

UniProt ID

Q13421

Alternative Names

Mesothelin; Pre-Pro-Megakaryocyte-Potentiating Factor; CAK1 Antigen; MPF; Soluble MPF Mesothelin Related Protein; Megakaryocyte Potentiating Factor; SMRP

Function

Membrane-anchored forms may play a role in cellular adhesion.

Megakaryocyte-potentiating factor (MPF) potentiates megakaryocyte colony formation in vitro.

Biological Process

Cell adhesion Source: UniProtKB
Cell-matrix adhesion Source: GO_Central
Pancreas development Source: Ensembl

Cellular Location

Plasma membrane
Cell membrane
Golgi apparatus
Megakaryocyte-potentiating factor:
Secreted
Isoform 3:
Secreted

Involvement in disease

Antibodies against MSLN are detected in patients with mesothelioma and ovarian cancer.

PTM

Both MPF and the cleaved form of mesothelin are N-glycosylated.
Proteolytically cleaved by a furin-like convertase to generate megakaryocyte-potentiating factor (MPF), and the cleaved form of mesothelin.

Hagerty, B. L., & Takabe, K. (2023). Biology of Mesothelin and Clinical Implications: A Review of Existing Literature. World Journal of Oncology, 14(5), 340.

Wang, Q., & Gong, R. (2022). Immunotherapy targeting mesothelin in acute myeloid leukemia. Journal of Leukocyte Biology, 112(4), 813-821.

Schoutrop, E., El-Serafi, I., Poiret, T., Zhao, Y., Gultekin, O., He, R., ... & Mattsson, J. (2021). Mesothelin-specific CAR T cells target ovarian cancer. Cancer Research, 81(11), 3022-3035.

Klampatsa, A., Dimou, V., & Albelda, S. M. (2021). Mesothelin-targeted CAR-T cell therapy for solid tumors. Expert opinion on biological therapy, 21(4), 473-486.

Weidemann, S., Gagelmann, P., Gorbokon, N., Lennartz, M., Menz, A., Luebke, A. M., ... & Simon, R. (2021). Mesothelin expression in human tumors: a tissue microarray study on 12,679 tumors. Biomedicines, 9(4), 397.

Kaeding, A. J., Barwe, S. P., Gopalakrishnapillai, A., Ries, R. E., Alonzo, T. A., Gerbing, R. B., ... & Meshinchi, S. (2021). Mesothelin is a novel cell surface disease marker and potential therapeutic target in acute myeloid leukemia. Blood Advances, 5(9), 2350-2361.

Montemagno, C., Cassim, S., Pouyssegur, J., Broisat, A., & Pagès, G. (2020). From malignant progression to therapeutic targeting: current insights of mesothelin in pancreatic ductal adenocarcinoma. International Journal of Molecular Sciences, 21(11), 4067.

Shen, J., Sun, X., & Zhou, J. (2020). Insights into the role of mesothelin as a diagnostic and therapeutic target in ovarian carcinoma. Frontiers in Oncology, 10, 1263.

Hagemann, U. B., Ellingsen, C., Schuhmacher, J., Kristian, A., Mobergslien, A., Cruciani, V., ... & Cuthbertson, A. S. (2019). Mesothelin-targeted thorium-227 conjugate (MSLN-TTC): preclinical evaluation of a new targeted alpha therapy for mesothelin-positive cancers. Clinical Cancer Research, 25(15), 4723-4734.

Lv, J., & Li, P. (2019). Mesothelin as a biomarker for targeted therapy. Biomarker Research, 7(1), 1-18.

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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme-Linked Immunospot (ELISpot)
Proteogenomics
Other Protocols

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Isotype control

For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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