Mouse Anti-MTDH Recombinant Antibody (CBT2855) (V2LY-0625-LY1909)

Name: Mouse Anti-MTDH Recombinant Antibody (CBT2855)
SKU: V2LY-0625-LY1909
Rating: 5 (1 reviews)

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Tested Data

Mouse Anti-MTDH Recombinant Antibody (CBT2855) (V2LY-0625-LY1909) in ELISA

Mouse Anti-MTDH Recombinant Antibody (CBT2855) (V2LY-0625-LY1909) in WB

Mouse Anti-MTDH Recombinant Antibody (CBT2855) (V2LY-0625-LY1909) in IF

Mouse Anti-MTDH Recombinant Antibody (CBT2855) (V2LY-0625-LY1909) in FC

Mouse Anti-MTDH Recombinant Antibody (CBT2855) (V2LY-0625-LY1909) in IHC

Datasheet

SDS

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Datasheet Target References Q & As Review & reward Protocols Associated Products

Basic Information

Host Animal

Mouse

Clone

CBT2855

Application

WB, IHC, ICC, FC

Immunogen

Purified recombinant fragment of human MTDH (AA: 200-450) expressed in mammalian.

Host Species

Mouse

Specificity

Human, Monkey

Antibody Isotype

IgG1

Clonality

Monoclonal Antibody

Application Notes

Application	Note
WB	1:500-1:2,000
IHC-P	1:200-1:1,000
ICC	1:50-1:200
FC	1:200-1:400
ELISA	1:10,000

Formulations & Storage [For reference only, actual COA shall prevail!]

Format

Liquid

Buffer

PBS

Preservative

Sodium azide

Concentration

Batch dependent

Purity

> 95% Purity determined by SDS-PAGE.

Storage

Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freezethaw cycles.

More Infomation

Target

Full Name

Metadherin

Entrez Gene ID

92140

UniProt ID

Q86UE4

Function

Down-regulates SLC1A2/EAAT2 promoter activity when expressed ectopically. Activates the nuclear factor kappa-B (NF-kappa-B) transcription factor. Promotes anchorage-independent growth of immortalized melanocytes and astrocytes which is a key component in tumor cell expansion. Promotes lung metastasis and also has an effect on bone and brain metastasis, possibly by enhancing the seeding of tumor cells to the target organ endothelium. Induces chemoresistance.

Biological Process

Lipopolysaccharide-mediated signaling pathway Source: UniProtKB
Negative regulation of apoptotic process Source: UniProtKB
Negative regulation of transcription by RNA polymerase II Source: UniProtKB
Positive regulation of angiogenesis Source: UniProtKB
Positive regulation of autophagy Source: UniProtKB
Positive regulation of I-kappaB kinase/NF-kappaB signaling Source: UniProtKB
Positive regulation of NF-kappaB transcription factor activity Source: UniProtKB
Positive regulation of protein kinase B signaling Source: UniProtKB
Regulation of transcription by RNA polymerase II Source: GO_Central

Cellular Location

Nucleus
Nucleus membrane
nucleolus
Endoplasmic reticulum
Endoplasmic reticulum membrane
Other locations
tight junction
perinuclear region
Note: In epithelial cells, recruited to tight junctions (TJ) during the maturation of the TJ complexes. A nucleolar staining may be due to nuclear targeting of an isoform lacking the transmembrane domain (By similarity). TNF-alpha causes translocation from the cytoplasm to the nucleus.

Topology

Lumenal: 1-48
Helical: 49-69
Cytoplasmic: 70-582

Ortiz-Soto, G., Babilonia-Díaz, N. S., Lacourt-Ventura, M. Y., Rivera-Rodríguez, D. M., Quiñones-Rodríguez, J. I., Colón-Vargas, M., ... & Martínez-Montemayor, M. M. (2023). Metadherin Regulates Inflammatory Breast Cancer Invasion and Metastasis. International Journal of Molecular Sciences, 24(5), 4694.

Wang, S. S., Zhai, G. Q., Chen, G., Huang, Z. G., Zhang, Y., Zhang, L. J., ... & Yan, H. B. (2023). Metadherin promotes the development of bladder cancer by enhancing cell division. Cancer Biotherapy & Radiopharmaceuticals, 38(9), 650-662.

Ghafar, M. T. A., & Soliman, N. A. (2022). Metadherin (AEG-1/MTDH/LYRIC) expression: significance in malignancy and crucial role in colorectal cancer. Advances in clinical chemistry, 106, 235-280.

Chen, Y., Huang, S., Guo, R., & Chen, D. (2021). Metadherin-mediated mechanisms in human malignancies. Biomarkers in Medicine, 15(18), 1769-1783.

Sultan, A., Sahar, N. E., Riaz, S. K., Qadir, J., Waqar, S. H., Haq, F., ... & Malik, M. F. A. (2021). Metadherin (MTDH) overexpression significantly correlates with advanced tumor grade and stages among colorectal cancer patients. Molecular Biology Reports, 48(12), 7999-8007.

Shen, M., Xie, S., Rowicki, M., Michel, S., Wei, Y., Hang, X., ... & Kang, Y. (2021). Therapeutic targeting of metadherin suppresses colorectal and lung cancer progression and metastasis. Cancer research, 81(4), 1014-1025.

Rong, C., Shi, Y., Huang, J., Wang, X., Shimizu, R., Mori, Y., ... & Liang, J. (2020). The effect of metadherin on NF-κB activation and downstream genes in ovarian cancer. Cell transplantation, 29, 0963689720905506.

Bi, J., Yang, S., Li, L., Dai, Q., Borcherding, N., Wagner, B. A., ... & Meng, X. (2019). Metadherin enhances vulnerability of cancer cells to ferroptosis. Cell death & disease, 10(10), 682.

Dhiman, G., Srivastava, N., Goyal, M., Rakha, E., Lothion-Roy, J., Mongan, N. P., ... & Baranwal, M. (2019). Metadherin: a therapeutic target in multiple cancers. Frontiers in oncology, 9, 349.

Zhang, Z., Qin, H., Jiang, B., Chen, W., Cao, W., Zhao, X., ... & Guo, H. (2019). miR‐30e‐5p suppresses cell proliferation and migration in bladder cancer through regulating metadherin. Journal of Cellular Biochemistry, 120(9), 15924-15932.

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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme Linked Immunospot (ELISpot)
Proteogenomic
Other Protocols

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Isotype control

For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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