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Mouse Anti-MTHFD2 Recombinant Antibody (4G7-2G3) (CBMAB-A5662-LY)


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Summary

Host Animal
Mouse
Specificity
Human
Clone
4G7-2G3
Antibody Isotype
IgG2a, κ
Application
WB, ELISA

Basic Information

Immunogen
MTHFD2 (AAH01548, 16 a.a. ~ 344 a.a) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
Specificity
Human
Antibody Isotype
IgG2a, κ
Clonality
Monoclonal
Application Notes
The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Formulations & Storage [For reference only, actual COA shall prevail!]

Format
Liquid
Purity
> 95% Purity determined by SDS-PAGE.
Storage
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freezethaw cycles.

Target

Full Name
Methylenetetrahydrofolate Dehydrogenase (NADP+ Dependent) 2, Methenyltetrahydrofolate Cyclohydrolase
Introduction
This gene encodes a nuclear-encoded mitochondrial bifunctional enzyme with methylenetetrahydrofolate dehydrogenase and methenyltetrahydrofolate cyclohydrolase activities. The enzyme functions as a homodimer and is unique in its absolute requirement for magnesium and inorganic phosphate. Formation of the enzyme-magnesium complex allows binding of NAD. Alternative splicing results in two different transcripts, one protein-coding and the other not protein-coding. This gene has a pseudogene on chromosome 7. [provided by RefSeq]
Entrez Gene ID
10797
UniProt ID
P13995
Alternative Names
NMDMC
Function
Although its dehydrogenase activity is NAD-specific, it can also utilize NADP at a reduced efficiency.
Biological Process
Folic acid metabolic process Source: Reactome
Tetrahydrofolate interconversion Source: GO_Central
Tetrahydrofolate metabolic process Source: UniProtKB
Cellular Location
Mitochondrion
More Infomation

Zhao, L. N., & Kaldis, P. (2022). The catalytic mechanism of the mitochondrial methylenetetrahydrofolate dehydrogenase/cyclohydrolase (MTHFD2). PLoS Computational Biology, 18(5), e1010140.

Sugiura, A., Andrejeva, G., Voss, K., Heintzman, D. R., Xu, X., Madden, M. Z., ... & Rathmell, J. C. (2022). MTHFD2 is a metabolic checkpoint controlling effector and regulatory T cell fate and function. Immunity, 55(1), 65-81.

Cuthbertson, C. R., Arabzada, Z., Bankhead III, A., Kyani, A., & Neamati, N. (2021). A review of small-molecule inhibitors of one-carbon enzymes: SHMT2 and MTHFD2 in the spotlight. ACS Pharmacology & Translational Science, 4(2), 624-646.

Li, G., Wu, J., Li, L., & Jiang, P. (2021). p53 deficiency induces MTHFD2 transcription to promote cell proliferation and restrain DNA damage. Proceedings of the National Academy of Sciences, 118(28), e2019822118.

Huang, J., Qin, Y., Lin, C., Huang, X., & Zhang, F. (2021). MTHFD2 facilitates breast cancer cell proliferation via the AKT signaling pathway. Experimental and Therapeutic Medicine, 22(1), 1-8.

Zhu, Z., & Leung, G. K. K. (2020). More than a metabolic enzyme: MTHFD2 as a novel target for anticancer therapy?. Frontiers in oncology, 10, 658.

Yu, C., Yang, L., Cai, M., Zhou, F., Xiao, S., Li, Y., ... & Huang, X. (2020). Down‐regulation of MTHFD2 inhibits NSCLC progression by suppressing cycle‐related genes. Journal of cellular and molecular medicine, 24(2), 1568-1577.

Green, N. H., Galvan, D. L., Badal, S. S., Chang, B. H., LeBleu, V. S., Long, J., ... & Danesh, F. R. (2019). MTHFD2 links RNA methylation to metabolic reprogramming in renal cell carcinoma. Oncogene, 38(34), 6211-6225.

Ju, H. Q., Lu, Y. X., Chen, D. L., Zuo, Z. X., Liu, Z. X., Wu, Q. N., ... & Xu, R. H. (2019). Modulation of redox homeostasis by inhibition of MTHFD2 in colorectal cancer: mechanisms and therapeutic implications. JNCI: Journal of the National Cancer Institute, 111(6), 584-596.

Kawai, J., Toki, T., Ota, M., Inoue, H., Takata, Y., Asahi, T., ... & Nakayama, K. (2019). Discovery of a potent, selective, and orally available MTHFD2 inhibitor (DS18561882) with in vivo antitumor activity. Journal of medicinal chemistry, 62(22), 10204-10220.

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For research use only. Not intended for any clinical use.

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