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Mouse Anti-MTHFD2 (AA 16-345) Recombinant Antibody (CBFYM-0508) (CBMAB-M0636-FY)

This product is mouse antibody that recognizes MTHFD2. The antibody CBFYM-0508 can be used for immunoassay techniques such as: ELISA, IHC, WB.
See all MTHFD2 antibodies

Summary

Host Animal
Mouse
Specificity
Human
Clone
CBFYM-0508
Antibody Isotype
IgG2a
Application
ELISA, IHC, WB

Basic Information

Specificity
Human
Antibody Isotype
IgG2a
Clonality
Monoclonal
Application Notes
The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Formulations & Storage [For reference only, actual COA shall prevail!]

Format
Liquid
Buffer
PBS, pH 7.2
Preservative
0.09% Sodium azide
Storage
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
Epitope
AA 16-345

Target

Full Name
Methylenetetrahydrofolate Dehydrogenase (NADP+ Dependent) 2, Methenyltetrahydrofolate Cyclohydrolase
Introduction
This gene encodes a nuclear-encoded mitochondrial bifunctional enzyme with methylenetetrahydrofolate dehydrogenase and methenyltetrahydrofolate cyclohydrolase activities. The enzyme functions as a homodimer and is unique in its absolute requirement for magnesium and inorganic phosphate. Formation of the enzyme-magnesium complex allows binding of NAD. Alternative splicing results in two different transcripts, one protein-coding and the other not protein-coding. This gene has a pseudogene on chromosome 7.
Entrez Gene ID
10797
UniProt ID
P13995
Alternative Names
Methylenetetrahydrofolate Dehydrogenase (NADP+ Dependent) 2, Methenyltetrahydrofolate Cyclohydrolase; NMDMC; Bifunctional Methylenetetrahydrofolate Dehydrogenase/Cyclohydrolase, Mitochondrial; NAD-Dependent Methylene Tetrahydrofolate Dehydrogenase Cyclohydrolase
Function
Although its dehydrogenase activity is NAD-specific, it can also utilize NADP at a reduced efficiency.
Biological Process
Folic acid metabolic process Source: Reactome
Tetrahydrofolate interconversion Source: GO_Central
Tetrahydrofolate metabolic process Source: UniProtKB
Cellular Location
Mitochondrion

Zhao, L. N., & Kaldis, P. (2022). The catalytic mechanism of the mitochondrial methylenetetrahydrofolate dehydrogenase/cyclohydrolase (MTHFD2). PLoS Computational Biology, 18(5), e1010140.

Sugiura, A., Andrejeva, G., Voss, K., Heintzman, D. R., Xu, X., Madden, M. Z., ... & Rathmell, J. C. (2022). MTHFD2 is a metabolic checkpoint controlling effector and regulatory T cell fate and function. Immunity, 55(1), 65-81.

Cuthbertson, C. R., Arabzada, Z., Bankhead III, A., Kyani, A., & Neamati, N. (2021). A review of small-molecule inhibitors of one-carbon enzymes: SHMT2 and MTHFD2 in the spotlight. ACS Pharmacology & Translational Science, 4(2), 624-646.

Li, G., Wu, J., Li, L., & Jiang, P. (2021). p53 deficiency induces MTHFD2 transcription to promote cell proliferation and restrain DNA damage. Proceedings of the National Academy of Sciences, 118(28), e2019822118.

Huang, J., Qin, Y., Lin, C., Huang, X., & Zhang, F. (2021). MTHFD2 facilitates breast cancer cell proliferation via the AKT signaling pathway. Experimental and Therapeutic Medicine, 22(1), 1-8.

Zhu, Z., & Leung, G. K. K. (2020). More than a metabolic enzyme: MTHFD2 as a novel target for anticancer therapy?. Frontiers in oncology, 10, 658.

Yu, C., Yang, L., Cai, M., Zhou, F., Xiao, S., Li, Y., ... & Huang, X. (2020). Down‐regulation of MTHFD2 inhibits NSCLC progression by suppressing cycle‐related genes. Journal of cellular and molecular medicine, 24(2), 1568-1577.

Green, N. H., Galvan, D. L., Badal, S. S., Chang, B. H., LeBleu, V. S., Long, J., ... & Danesh, F. R. (2019). MTHFD2 links RNA methylation to metabolic reprogramming in renal cell carcinoma. Oncogene, 38(34), 6211-6225.

Ju, H. Q., Lu, Y. X., Chen, D. L., Zuo, Z. X., Liu, Z. X., Wu, Q. N., ... & Xu, R. H. (2019). Modulation of redox homeostasis by inhibition of MTHFD2 in colorectal cancer: mechanisms and therapeutic implications. JNCI: Journal of the National Cancer Institute, 111(6), 584-596.

Kawai, J., Toki, T., Ota, M., Inoue, H., Takata, Y., Asahi, T., ... & Nakayama, K. (2019). Discovery of a potent, selective, and orally available MTHFD2 inhibitor (DS18561882) with in vivo antitumor activity. Journal of medicinal chemistry, 62(22), 10204-10220.

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For research use only. Not intended for any clinical use.

Custom Antibody Labeling

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