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Mouse Anti-MUC4 Recombinant Antibody (5B12) (CBMAB-A5688-LY)

The product is antibody recognizes MUC4. The antibody 5B12 immunoassay techniques such as: WB, ELISA.
See all MUC4 antibodies
Published Data

Summary

Host Animal
Mouse
Specificity
Human
Clone
5B12
Antibody Isotype
IgG2a, κ
Application
WB, ELISA

Basic Information

Immunogen
MUC4 (NP_004523, 79 a.a. ~ 188 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
Specificity
Human
Antibody Isotype
IgG2a, κ
Clonality
Monoclonal
Application Notes
The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Formulations & Storage [For reference only, actual COA shall prevail!]

Format
Liquid
Purity
> 95% Purity determined by SDS-PAGE.
Storage
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freezethaw cycles.

Target

Full Name
mucin 4, cell surface associated
Introduction
The major constituents of mucus, the viscous secretion that covers epithelial surfaces such as those in the trachea, colon, and cervix, are highly glycosylated proteins called mucins. These glycoproteins play important roles in the protection of the epithelial cells and have been implicated in epithelial renewal and differentiation. This gene encodes an integral membrane glycoprotein found on the cell surface, although secreted isoforms may exist. At least two dozen transcript variants of this gene have been found, although for many of them the full-length transcript has not been determined or they are found only in tumor tissues. This gene contains a region in the coding sequence which has a variable number (>100) of 48 nt tandem repeats. [provided by RefSeq]
Entrez Gene ID
UniProt ID
Alternative Names
HSA276359
Function
Membrane-bound mucin, a family of highly glycosylated proteins that constitute the major component of the mucus, the slimy and viscous secretion covering epithelial surfaces (PubMed:10880978).

These glycoproteins play important roles in the protection of the epithelium and are implicated in epithelial renewal and differentiation (PubMed:10880978).

Regulates cellular behavior through both anti-adhesive effects on cell-cell and cell-extracellular matrix interactions and its ability to act as an intramembrane ligand for ERBB2. Plays an important role in proliferation and differentiation of epithelial cells by inducing specific phosphorylation of ERBB2. In polarized epithelial cells, segregates ERBB2 and other ERBB receptors and prevents ERBB2 from acting as a coreceptor. The interaction with ERBB2 leads to enhanced expression of CDKN1B. The formation of a MUC4-ERBB2-ERBB3-NRG1 complex leads to down-regulation of CDKN1B, resulting in repression of apoptosis and stimulation of proliferation. Its ability to promote tumor growth may be mainly due to repression of apoptosis as opposed to proliferation.
Biological Process
Cell-matrix adhesion Source: InterPro
Maintenance of gastrointestinal epithelium Source: UniProtKB
Regulation of signaling receptor activity Source: GO_Central
Cellular Location
Chain Mucin-4 beta chain:
Plasma membrane
Cell membrane
Note: Isoforms lacking the Cys-rich region, EGF-like domains and transmembrane region are secreted. Secretion occurs by splicing or proteolytic processing.
Chain Mucin-4 alpha chain:
Plasma membrane
Cell membrane
Secreted
Note: Forms a complex with Mucin-4 beta chain at the cell membrane.
Isoform 3:
Plasma membrane
Cell membrane
Isoform 11:
Secreted
Isoform 15:
Secreted
Isoform 17:
Plasma membrane
Cell membrane
Topology
Helical: 5369-5389
PTM
Proteolytically cleaved into 2 chains, mucin-4 alpha chain and mucin-4 beta chain.
Mucin-4 alpha chain:
Highly O-glycosylated.
Mucin-4 beta chain:
Is predominantly N-glycosylated.

Martinez-Carrasco, R., Rachagani, S., Batra, S. K., Argüeso, P., & Fini, M. E. (2023). Roles unveiled for membrane-associated mucins at the ocular surface using a Muc4 knockout mouse model. Scientific reports, 13(1), 13558.

Arruda, B. L., Kanefsky, R. A., Hau, S., Janzen, G. M., Anderson, T. K., & Baker, A. L. V. (2023). Mucin 4 is a cellular biomarker of necrotizing bronchiolitis in influenza A virus infection. Microbes and Infection, 105169.

Dreyer, C. A., VanderVorst, K., Free, S., Rowson-Hodel, A., & Carraway, K. L. (2022). The role of membrane mucin MUC4 in breast cancer metastasis. Endocrine-related cancer, 29(1), R17-R32.

Kim, J. H., Park, H. S., Lee, J. Y., Ko, E. J., Kim, Y. R., Cho, H. Y., ... & Kim, N. K. (2022). Association study between Mucin 4 (MUC4) polymorphisms and idiopathic recurrent pregnancy loss in a Korean population. Genes, 13(6), 937.

Sagar, S., Leiphrakpam, P. D., Thomas, D., McAndrews, K. L., Caffrey, T. C., Swanson, B. J., ... & Radhakrishnan, P. (2021). MUC4 enhances gemcitabine resistance and malignant behaviour in pancreatic cancer cells expressing cancer-associated short O-glycans. Cancer letters, 503, 91-102.

Peng, L., Li, Y., Gu, H., Xiang, L., Xiong, Y., Wang, R., ... & Wang, J. (2021). Mucin 4 mutation is associated with tumor mutation burden and promotes antitumor immunity in colon cancer patients. Aging (Albany NY), 13(6), 9043.

Yu, J., Xu, L., Yan, J., Yu, J., Wu, X., Dai, J., ... & Kong, Y. (2020). MUC4 isoforms expression profiling and prognosis value in Chinese melanoma patients. Clinical and Experimental Medicine, 20, 299-311.

Gautam, S. K., Kumar, S., Dam, V., Ghersi, D., Jain, M., & Batra, S. K. (2020, February). MUCIN-4 (MUC4) is a novel tumor antigen in pancreatic cancer immunotherapy. In Seminars in immunology (Vol. 47, p. 101391). Academic Press.

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For research use only. Not intended for any clinical use.

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