Mouse Anti-MUC4 Recombinant Antibody (CBFYM-2809) (CBMAB-M3002-FY)

Name: Mouse Anti-MUC4 Recombinant Antibody (CBFYM-2809)
SKU: CBMAB-M3002-FY
Rating: 5 (1 reviews)

See all MUC4 antibodies

Compare Online Inquiry

Datasheet

SDS

Request for COA

Datasheet Target References Q & As Review & reward Protocols Associated Products

Basic Information

Host Animal

Mouse

Clone

CBFYM-2809

Application

IHC-Fr, IHC-P

Immunogen

Rat ASGP-2

Specificity

Human, Rat

Antibody Isotype

IgG1

Clonality

Monoclonal

Application Notes

The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Formulations & Storage [For reference only, actual COA shall prevail!]

Format

Liquid

Buffer

PBS, pH 7.4, 1% BSA

Preservative

0.1% Sodium azide

Concentration

0.1 mg/mL

Storage

Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

More Infomation

Target

Full Name

mucin 4, cell surface associated

Introduction

The major constituents of mucus, the viscous secretion that covers epithelial surfaces such as those in the trachea, colon, and cervix, are highly glycosylated proteins called mucins. These glycoproteins play important roles in the protection of the epithelial cells and have been implicated in epithelial renewal and differentiation. This gene encodes an integral membrane glycoprotein found on the cell surface, although secreted isoforms may exist. At least two dozen transcript variants of this gene have been found, although for many of them the full-length transcript has not been determined or they are found only in tumor tissues. This gene contains a region in the coding sequence which has a variable number of 48 nt tandem repeats.

Entrez Gene ID

Human	4585
Rat	303887

UniProt ID

Human	Q99102
Rat	Q63661

Alternative Names

Mucin 4, Cell Surface Associated; Pancreatic Adenocarcinoma Mucin; Mucin 4, Tracheobronchial; Ascites Sialoglycoprotein; Tracheobronchial Mucin; Testis Mucin

Function

Membrane-bound mucin, a family of highly glycosylated proteins that constitute the major component of the mucus, the slimy and viscous secretion covering epithelial surfaces (PubMed:10880978).

These glycoproteins play important roles in the protection of the epithelium and are implicated in epithelial renewal and differentiation (PubMed:10880978).

Regulates cellular behavior through both anti-adhesive effects on cell-cell and cell-extracellular matrix interactions and its ability to act as an intramembrane ligand for ERBB2. Plays an important role in proliferation and differentiation of epithelial cells by inducing specific phosphorylation of ERBB2. In polarized epithelial cells, segregates ERBB2 and other ERBB receptors and prevents ERBB2 from acting as a coreceptor. The interaction with ERBB2 leads to enhanced expression of CDKN1B. The formation of a MUC4-ERBB2-ERBB3-NRG1 complex leads to down-regulation of CDKN1B, resulting in repression of apoptosis and stimulation of proliferation. Its ability to promote tumor growth may be mainly due to repression of apoptosis as opposed to proliferation.

Biological Process

Cell-matrix adhesion Source: InterPro
Maintenance of gastrointestinal epithelium Source: UniProtKB
Regulation of signaling receptor activity Source: GO_Central

Cellular Location

Chain Mucin-4 beta chain:
Plasma membrane
Cell membrane
Note: Isoforms lacking the Cys-rich region, EGF-like domains and transmembrane region are secreted. Secretion occurs by splicing or proteolytic processing.
Chain Mucin-4 alpha chain:
Plasma membrane
Cell membrane
Secreted
Note: Forms a complex with Mucin-4 beta chain at the cell membrane.
Isoform 3:
Plasma membrane
Cell membrane
Isoform 11:
Secreted
Isoform 15:
Secreted
Isoform 17:
Plasma membrane
Cell membrane

Topology

Helical: 5369-5389

PTM

Proteolytically cleaved into 2 chains, mucin-4 alpha chain and mucin-4 beta chain.
Mucin-4 alpha chain:
Highly O-glycosylated.
Mucin-4 beta chain:
Is predominantly N-glycosylated.

Martinez-Carrasco, R., Rachagani, S., Batra, S. K., Argüeso, P., & Fini, M. E. (2023). Roles unveiled for membrane-associated mucins at the ocular surface using a Muc4 knockout mouse model. Scientific reports, 13(1), 13558.

Arruda, B. L., Kanefsky, R. A., Hau, S., Janzen, G. M., Anderson, T. K., & Baker, A. L. V. (2023). Mucin 4 is a cellular biomarker of necrotizing bronchiolitis in influenza A virus infection. Microbes and Infection, 105169.

Dreyer, C. A., VanderVorst, K., Free, S., Rowson-Hodel, A., & Carraway, K. L. (2022). The role of membrane mucin MUC4 in breast cancer metastasis. Endocrine-related cancer, 29(1), R17-R32.

Kim, J. H., Park, H. S., Lee, J. Y., Ko, E. J., Kim, Y. R., Cho, H. Y., ... & Kim, N. K. (2022). Association study between Mucin 4 (MUC4) polymorphisms and idiopathic recurrent pregnancy loss in a Korean population. Genes, 13(6), 937.

Sagar, S., Leiphrakpam, P. D., Thomas, D., McAndrews, K. L., Caffrey, T. C., Swanson, B. J., ... & Radhakrishnan, P. (2021). MUC4 enhances gemcitabine resistance and malignant behaviour in pancreatic cancer cells expressing cancer-associated short O-glycans. Cancer letters, 503, 91-102.

Peng, L., Li, Y., Gu, H., Xiang, L., Xiong, Y., Wang, R., ... & Wang, J. (2021). Mucin 4 mutation is associated with tumor mutation burden and promotes antitumor immunity in colon cancer patients. Aging (Albany NY), 13(6), 9043.

Yu, J., Xu, L., Yan, J., Yu, J., Wu, X., Dai, J., ... & Kong, Y. (2020). MUC4 isoforms expression profiling and prognosis value in Chinese melanoma patients. Clinical and Experimental Medicine, 20, 299-311.

Gautam, S. K., Kumar, S., Dam, V., Ghersi, D., Jain, M., & Batra, S. K. (2020, February). MUCIN-4 (MUC4) is a novel tumor antigen in pancreatic cancer immunotherapy. In Seminars in immunology (Vol. 47, p. 101391). Academic Press.

Ask a question We look forward to hearing from you.

0 reviews or Q&As

Purchasing FAQs
Technical FAQs

Have you used Mouse Anti-MUC4 Recombinant Antibody (CBFYM-2809)?
Submit a review and get a Coupon or an Amazon gift card. 20% off Coupon

$30 eGift Card
Submit a review

Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme Linked Immunospot (ELISpot)
Proteogenomic
Other Protocols

Related Products

Mouse Anti-MUC4 (AA 79-189) Recombinant Antibody (CBFYM-2808) (CAT#: CBMAB-M3001-FY)

Mouse Anti-MUC4 Recombinant Antibody (CBFYM-0205) (CAT#: CBMAB-M0247-FY)

Mouse Anti-MUC4 Recombinant Antibody (5B12) (CAT#: CBMAB-A5688-LY)

Mouse Anti-MUC4 Recombinant Antibody (CBYC-A1002) (CAT#: CBMAB-A4586-YC)

Mouse Anti-MUC4 Recombinant Antibody (CBFYM-0252) (CAT#: CBMAB-M0315-FY)

Mouse Anti-MUC4 Recombinant Antibody (CBFYM-0748) (CAT#: CBMAB-M0890-FY)

Mouse Anti-MUC4 Recombinant Antibody (4F12) (CAT#: CBMAB-0222-CN)

Mouse Anti-MUC4 Recombinant Antibody (CBFYM-2810) (CAT#: CBMAB-M3003-FY)

Mouse Anti-MUC4 Recombinant Antibody (CBFYM-0747) (CAT#: CBMAB-M0889-FY)

Isotype control

For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

Online Inquiry

Request bulk or custom quote Second antibody and isotype control

Contact us

Tel: (USA)
(UK)
Fax:

Global Locations

Email:

Online Inquiry