Human MUC4 ELISA Kit (V2LY-0626-LY2174)

Name: Human MUC4 ELISA Kit
SKU: V2LY-0626-LY2174
Rating: 5 (1 reviews)

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Tested Data

Datasheet

SDS

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Datasheet Target References Q & As Review & reward Protocols Associated Products

Basic Information

Sensitivity

0.0055 ng/mL

Detection Range

0.01-3 ng/mL

Sample Type

Serum, Plasma, cell culture supernates

Specificity

Human

Assay Type

Sandwich

Reactivity

Human

Assay Time

1.5 h

Molecule Mass

231.5 kDa

Components

Pre-coated ELISA Plate: 12 wells * 8 detachable strips
Standard solution: 0.5ml x1
Standard diluent: 3ml x1
Streptavidin-HRP: 6ml x1
Stop solution: 6ml x1
Substrate solution A: 6ml x1
Substrate solution B: 6ml x1
Wash buffer concentrate (25x): 20ml x1
Biotinylated antibody: 1ml x1

Formulations & Storage [For reference only, actual COA shall prevail!]

Storage

Store at 2-8°C

More Infomation

Target

Full Name

mucin 4, cell surface associated

Function

Membrane-bound mucin, a family of highly glycosylated proteins that constitute the major component of the mucus, the slimy and viscous secretion covering epithelial surfaces (PubMed:10880978).

These glycoproteins play important roles in the protection of the epithelium and are implicated in epithelial renewal and differentiation (PubMed:10880978).

Regulates cellular behavior through both anti-adhesive effects on cell-cell and cell-extracellular matrix interactions and its ability to act as an intramembrane ligand for ERBB2. Plays an important role in proliferation and differentiation of epithelial cells by inducing specific phosphorylation of ERBB2. In polarized epithelial cells, segregates ERBB2 and other ERBB receptors and prevents ERBB2 from acting as a coreceptor. The interaction with ERBB2 leads to enhanced expression of CDKN1B. The formation of a MUC4-ERBB2-ERBB3-NRG1 complex leads to down-regulation of CDKN1B, resulting in repression of apoptosis and stimulation of proliferation. Its ability to promote tumor growth may be mainly due to repression of apoptosis as opposed to proliferation.

Biological Process

Cell-matrix adhesion Source: InterPro
Maintenance of gastrointestinal epithelium Source: UniProtKB
Regulation of signaling receptor activity Source: GO_Central

Cellular Location

Chain Mucin-4 beta chain:
Plasma membrane
Cell membrane
Note: Isoforms lacking the Cys-rich region, EGF-like domains and transmembrane region are secreted. Secretion occurs by splicing or proteolytic processing.
Chain Mucin-4 alpha chain:
Plasma membrane
Cell membrane
Secreted
Note: Forms a complex with Mucin-4 beta chain at the cell membrane.
Isoform 3:
Plasma membrane
Cell membrane
Isoform 11:
Secreted
Isoform 15:
Secreted
Isoform 17:
Plasma membrane
Cell membrane

Topology

Helical: 5369-5389

PTM

Proteolytically cleaved into 2 chains, mucin-4 alpha chain and mucin-4 beta chain.
Mucin-4 alpha chain:
Highly O-glycosylated.
Mucin-4 beta chain:
Is predominantly N-glycosylated.

Martinez-Carrasco, R., Rachagani, S., Batra, S. K., Argüeso, P., & Fini, M. E. (2023). Roles unveiled for membrane-associated mucins at the ocular surface using a Muc4 knockout mouse model. Scientific reports, 13(1), 13558.

Arruda, B. L., Kanefsky, R. A., Hau, S., Janzen, G. M., Anderson, T. K., & Baker, A. L. V. (2023). Mucin 4 is a cellular biomarker of necrotizing bronchiolitis in influenza A virus infection. Microbes and Infection, 105169.

Dreyer, C. A., VanderVorst, K., Free, S., Rowson-Hodel, A., & Carraway, K. L. (2022). The role of membrane mucin MUC4 in breast cancer metastasis. Endocrine-related cancer, 29(1), R17-R32.

Kim, J. H., Park, H. S., Lee, J. Y., Ko, E. J., Kim, Y. R., Cho, H. Y., ... & Kim, N. K. (2022). Association study between Mucin 4 (MUC4) polymorphisms and idiopathic recurrent pregnancy loss in a Korean population. Genes, 13(6), 937.

Sagar, S., Leiphrakpam, P. D., Thomas, D., McAndrews, K. L., Caffrey, T. C., Swanson, B. J., ... & Radhakrishnan, P. (2021). MUC4 enhances gemcitabine resistance and malignant behaviour in pancreatic cancer cells expressing cancer-associated short O-glycans. Cancer letters, 503, 91-102.

Peng, L., Li, Y., Gu, H., Xiang, L., Xiong, Y., Wang, R., ... & Wang, J. (2021). Mucin 4 mutation is associated with tumor mutation burden and promotes antitumor immunity in colon cancer patients. Aging (Albany NY), 13(6), 9043.

Yu, J., Xu, L., Yan, J., Yu, J., Wu, X., Dai, J., ... & Kong, Y. (2020). MUC4 isoforms expression profiling and prognosis value in Chinese melanoma patients. Clinical and Experimental Medicine, 20, 299-311.

Gautam, S. K., Kumar, S., Dam, V., Ghersi, D., Jain, M., & Batra, S. K. (2020, February). MUCIN-4 (MUC4) is a novel tumor antigen in pancreatic cancer immunotherapy. In Seminars in immunology (Vol. 47, p. 101391). Academic Press.

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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme Linked Immunospot (ELISpot)
Proteogenomic
Other Protocols

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Isotype control

For research use only. Not intended for any clinical use.

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