Sign in or Register   Sign in or Register
  |  

Mouse Anti-MUC5AC Recombinant Antibody (2H7) (CBMAB-A5690-LY)

The product is antibody recognizes MUC5AC. The antibody 2H7 immunoassay techniques such as: WB, ELISA.
See all MUC5AC antibodies
Published Data
Fig.1 Study of the expression of MUC2 and MUC5AC apomucins in NCI-H292 cells and effect of TGF-α on their expression by immunohistochemistry.
...View More

Summary

Host Animal
Mouse
Specificity
Human
Clone
2H7
Antibody Isotype
IgG1, κ
Application
WB, ELISA

Basic Information

Immunogen
MUC5AC (XP_495860, 351 a.a. ~ 450 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
Specificity
Human
Antibody Isotype
IgG1, κ
Clonality
Monoclonal
Application Notes
The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Formulations & Storage [For reference only, actual COA shall prevail!]

Format
Liquid
Purity
> 95% Purity determined by SDS-PAGE.
Storage
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freezethaw cycles.

Target

Full Name
Mucin 5AC, Oligomeric Mucus/Gel-Forming
Entrez Gene ID
4586
UniProt ID
P98088
Alternative Names
MUC5
Function
Gel-forming glycoprotein of gastric and respiratory tract epithelia that protects the mucosa from infection and chemical damage by binding to inhaled microorganisms and particles that are subsequently removed by the mucociliary system (PubMed:14535999, PubMed:14718370).

Interacts with H.pylori in the gastric epithelium, Barrett's esophagus as well as in gastric metaplasia of the duodenum (GMD) (PubMed:14535999).
Biological Process
Phosphatidylinositol-mediated signaling Source: UniProtKB
Cellular Location
Secreted
PTM
C-, O- and N-glycosylated (PubMed:14718370). O-glycosylated on the second and last Thr of the Thr-/Ser-rich tandem repeats TTPSPVPTTSTTSA (PubMed:25939779, PubMed:14718370, PubMed:22186971). One form of glycosylation is also known as Lewis B (LeB) blood group antigen, a tetrasaccharide consisting of N-acetylglucosamine having a fucosyl residue attached (PubMed:14535999). It has a role as an epitope and antigen and functions as a receptor for H.pylori binding and facilitates infection (PubMed:14535999). C-mannosylation in the Cys-rich subdomains may be required for proper folding of these regions and for export from the endoplasmic reticulum during biosynthesis (PubMed:14718370).
Proteolytic cleavage in the C-terminal is initiated early in the secretory pathway and does not involve a serine protease. The extent of cleavage is increased in the acidic parts of the secretory pathway. Cleavage generates a reactive group which could link the protein to a primary amide.

Song, D., Iverson, E., Kaler, L., Boboltz, A., Scull, M. A., & Duncan, G. A. (2022). MUC5B mobilizes and MUC5AC spatially aligns mucociliary transport on human airway epithelium. Science Advances, 8(47), eabq5049.

Tajiri, T., Matsumoto, H., Jinnai, M., Kanemitsu, Y., Nagasaki, T., Iwata, T., ... & Niimi, A. (2022). Pathophysiological relevance of sputum MUC5AC and MUC5B levels in patients with mild asthma. Allergology International, 71(2), 193-199.

Rico, S. D., Mahnken, M., Büscheck, F., Dum, D., Luebke, A. M., Kluth, M., ... & Marx, A. H. (2021). MUC5AC expression in various tumor types and nonneoplastic tissue: a tissue microarray study on 10 399 tissue samples. Technology in Cancer Research & Treatment, 20, 15330338211043328.

Carpenter, J., Wang, Y., Gupta, R., Li, Y., Haridass, P., Subramani, D. B., ... & Kesimer, M. (2021). Assembly and organization of the N-terminal region of mucin MUC5AC: Indications for structural and functional distinction from MUC5B. Proceedings of the National Academy of Sciences, 118(39), e2104490118.

Radicioni, G., Ceppe, A., Ford, A. A., Alexis, N. E., Barr, R. G., Bleecker, E. R., ... & Kesimer, M. (2021). Airway mucin MUC5AC and MUC5B concentrations and the initiation and progression of chronic obstructive pulmonary disease: an analysis of the SPIROMICS cohort. The Lancet Respiratory Medicine, 9(11), 1241-1254.

Manne, A., Esnakula, A., Abushahin, L., & Tsung, A. (2021). Understanding the clinical impact of MUC5AC expression on pancreatic ductal adenocarcinoma. Cancers, 13(12), 3059.

Li, J., & Ye, Z. (2020). The potential role and regulatory mechanisms of MUC5AC in chronic obstructive pulmonary disease. Molecules, 25(19), 4437.

Olli, K. E., Rapp, C., O’Connell, L., Collins, C. B., McNamee, E. N., Jensen, O., ... & Aherne, C. M. (2020). Muc5ac expression protects the colonic barrier in experimental colitis. Inflammatory bowel diseases, 26(9), 1353-1367.

Samsuzzaman, M., Uddin, M. S., Shah, M. A., & Mathew, B. (2019). Natural inhibitors on airway mucin: Molecular insight into the therapeutic potential targeting MUC5AC expression and production. Life sciences, 231, 116485.

Okuda, K., Chen, G., Subramani, D. B., Wolf, M., Gilmore, R. C., Kato, T., ... & Boucher, R. C. (2019). Localization of secretory mucins MUC5AC and MUC5B in normal/healthy human airways. American journal of respiratory and critical care medicine, 199(6), 715-727.

Ask a question We look forward to hearing from you.
0 reviews or Q&As

Purchasing FAQs
Technical FAQs

Loading...
Have you used Mouse Anti-MUC5AC Recombinant Antibody (2H7)?
Submit a review and get a Coupon or an Amazon gift card. 20% off Coupon $30 eGift Card
Submit a review
Loading...
For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

Online Inquiry

Documents

Contact us

  • Tel: (USA)
  • (UK)
  • Fax:
  • Email:

Submit A Review

Go to
Compare