Mouse Anti-MYCN Recombinant Antibody (CBFYM-0763) (CBMAB-M0906-FY)

Name: Mouse Anti-MYCN Recombinant Antibody (CBFYM-0763)
SKU: CBMAB-M0906-FY
Rating: 5 (1 reviews)

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Datasheet

SDS

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Datasheet Target References Q & As Review & reward Protocols Associated Products

Basic Information

Host Animal

Mouse

Clone

CBFYM-0763

Application

IHC, WB

Specificity

Human

Antibody Isotype

IgG2b

Clonality

Monoclonal

Application Notes

The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Formulations & Storage [For reference only, actual COA shall prevail!]

Format

Liquid

Buffer

PBS, pH 7.2

Storage

Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

More Infomation

Target

Full Name

MYCN

Introduction

This gene is a member of the MYC family and encodes a protein with a basic helix-loop-helix domain. This protein is located in the nucleus and must dimerize with another bHLH protein in order to bind DNA. Amplification of this gene is associated with a variety of tumors, most notably neuroblastomas. Multiple alternatively spliced transcript variants encoding different isoforms have been found for this gene.

Entrez Gene ID

4613

UniProt ID

P04198

Alternative Names

MYCN Proto-Oncogene, BHLH Transcription Factor; V-Myc Avian Myelocytomatosis Viral Oncogene Neuroblastoma Derived Homolog; Class E Basic Helix-Loop-Helix Protein 37; BHLHe37; NMYC; Neuroblastoma-Derived V-Myc Avian Myelocytomatosis Viral Related Oncogene; N-Myc Proto-Oncogene Protein

Function

Positively regulates the transcription of MYCNOS in neuroblastoma cells.

Biological Process

Negative regulation of gene expression Source: BHF-UCL
Positive regulation of gene expression Source: BHF-UCL
Positive regulation of production of miRNAs involved in gene silencing by miRNA Source: BHF-UCL
Positive regulation of transcription, DNA-templated Source: UniProtKB
Positive regulation of transcription by RNA polymerase II Source: NTNU_SB
Regulation of transcription by RNA polymerase II Source: GO_Central

Cellular Location

Nucleus

Involvement in disease

Amplification of the N-MYC gene is associated with a variety of human tumors, most frequently neuroblastoma, where the level of amplification appears to increase as the tumor progresses.
Feingold syndrome 1 (FGLDS1):
A syndrome characterized by variable combinations of esophageal and duodenal atresias, microcephaly, learning disability, mental retardation, and limb malformations. Hand and foot abnormalities may include hypoplastic thumbs, clinodactyly of second and fifth fingers, syndactyly (characteristically between second and third and fourth and fifth toes), and shortened or absent middle phalanges. Cardiac and renal malformations, vertebral anomalies, and deafness have also been described.

PTM

Phosphorylated by GSK3-beta which may promote its degradation (PubMed:24391509). Phosphorylated by AURKA (PubMed:27837025).

Bhardwaj, N., Das, G., & Srinivasan, R. (2023). Neuroblastoma-derived v-myc avian myelocytomatosis viral related oncogene or MYCN gene. Journal of Clinical Pathology.

Pouliou, M., Koutsi, M. A., Champezou, L., Giannopoulou, A. I., Vatsellas, G., Piperi, C., & Agelopoulos, M. (2023). MYCN Amplifications and Metabolic Rewiring in Neuroblastoma. Cancers, 15(19), 4803.

Qin, X., & Chen, B. (2023). Comprehensive analysis and validation reveal potential MYCN regulatory biomarkers associated with neuroblastoma prognosis. Journal of Biomolecular Structure and Dynamics, 41(18), 8902-8917.

Liu, R., Shi, P., Wang, Z., Yuan, C., & Cui, H. (2021). Molecular mechanisms of MYCN dysregulation in cancers. Frontiers in Oncology, 10, 625332.

Braoudaki, M., Hatziagapiou, K., Zaravinos, A., & Lambrou, G. I. (2021). MYCN in neuroblastoma:“old wine into new wineskins”. Diseases, 9(4), 78.

Fu, Y., Yuan, S. S., Zhang, L. J., Ji, Z. L., & Quan, X. J. (2020). Atonal bHLH transcription factor 1 is an important factor for maintaining the balance of cell proliferation and differentiation in tumorigenesis. Oncology letters, 20(3), 2595-2605.

Huang, X., Zhao, J., Zhu, J., Chen, S., Fu, W., Tian, X., ... & Zhou, H. (2019). MYCN gene polymorphisms and Wilms tumor susceptibility in Chinese children. Journal of Clinical Laboratory Analysis, 33(9), e22988.

Aygun, N., & Altungoz, O. (2019). MYCN is amplified during S phase, and c‑myb is involved in controlling MYCN expression and amplification in MYCN‑amplified neuroblastoma cell lines. Molecular medicine reports, 19(1), 345-361.

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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme-Linked Immunospot (ELISpot)
Proteogenomics
Other Protocols

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Isotype control

For research use only. Not intended for any clinical use.

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