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Mouse Anti-MYF5 (AA 1-255) Recombinant Antibody (CBFYM-0174) (CBMAB-M0201-FY)

This product is mouse antibody that recognizes MYF5. The antibody CBFYM-0174 can be used for immunoassay techniques such as: ELISA, ICC.
See all MYF5 antibodies

Summary

Host Animal
Mouse
Specificity
Human
Clone
CBFYM-0174
Antibody Isotype
IgG2a
Application
ELISA, ICC

Basic Information

Immunogen
Recombinant protein corresponding to aa1-255 from human MYF-5 expressed in E. coli
Specificity
Human
Antibody Isotype
IgG2a
Clonality
Monoclonal
Application Notes
The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Formulations & Storage [For reference only, actual COA shall prevail!]

Format
Lyophilized
Storage
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
Epitope
AA 1-255

Target

Full Name
MYOGENIC FACTOR 5
Introduction
MYF5 is a Protein Coding gene. Diseases associated with MYF5 include Rhabdomyosarcoma and Skeletal Muscle Cancer. Among its related pathways are miRs in Muscle Cell Differentiation and Myogenesis. Gene Ontology annotations related to this gene include sequence-specific DNA binding and RNA polymerase II proximal promoter sequence-specific DNA binding. An important paralog of this gene is MYOD1.
Entrez Gene ID
4617
UniProt ID
P13349
Alternative Names
Myogenic Factor 5; Class C Basic Helix-Loop-Helix Protein 2; BHLHc2; Myf-5
Function
Transcriptional activator that promotes transcription of muscle-specific target genes and plays a role in muscle differentiation (PubMed:29887215).

Together with MYOG and MYOD1, co-occupies muscle-specific gene promoter core region during myogenesis. Induces fibroblasts to differentiate into myoblasts. Probable sequence specific DNA-binding protein.
Biological Process
Camera-type eye development Source: Ensembl
Cartilage condensation Source: Ensembl
Embryonic skeletal system morphogenesis Source: Ensembl
Extracellular matrix organization Source: Ensembl
Muscle cell fate commitment Source: BHF-UCL
Muscle organ development Source: ProtInc
Muscle tissue morphogenesis Source: Ensembl
Ossification Source: Ensembl
Positive regulation of myoblast differentiation Source: GO_Central
Positive regulation of skeletal muscle fiber development Source: GO_Central
Regulation of cell-matrix adhesion Source: Ensembl
Regulation of transcription by RNA polymerase II Source: GO_Central
Skeletal muscle cell differentiation Source: GO_Central
Skeletal muscle tissue development Source: ProtInc
Somitogenesis Source: Ensembl
Cellular Location
Nucleus
Involvement in disease
Ophthalmoplegia, external, with rib and vertebral anomalies (EORVA):
An autosomal recessive disorder characterized by congenital nonprogressive external ophthalmoplegia, ptosis, scoliosis, torticollis, and vertebral and rib anomalies.

Shi, L. L., Zhu, K. C., & Wang, H. L. (2022). Characterization of myogenic regulatory factors, myod and myf5 from Megalobrama amblycephala and the effect of lipopolysaccharide on satellite cells in skeletal muscle. Gene, 834, 146608.

Dong, L., Wang, M., Gao, X., Zheng, X., Zhang, Y., Sun, L., ... & Wang, Y. (2022). miR-9-5p promotes myogenic differentiation via the Dlx3/Myf5 axis. PeerJ, 10, e13360.

Maeng, G., Das, S., Greising, S. M., Gong, W., Singh, B. N., Kren, S., ... & Garry, M. G. (2021). Humanized skeletal muscle in MYF5/MYOD/MYF6-null pig embryos. Nature Biomedical Engineering, 5(8), 805-814.

Zhao, C., Raza, S. H. A., Khan, R., Sabek, A., Khan, S., Ullah, I., ... & Zan, L. (2020). Genetic variants in MYF5 affected growth traits and beef quality traits in Chinese Qinchuan cattle. Genomics, 112(4), 2804-2812.

Osborn, D. P., Li, K., Cutty, S. J., Nelson, A. C., Wardle, F. C., Hinits, Y., & Hughes, S. M. (2020). Fgf-driven Tbx protein activities directly induce myf5 and myod to initiate zebrafish myogenesis. Development, 147(8), dev184689.

Cai, S., Zhu, Q., Guo, C., Yuan, R., Zhang, X., Nie, Y., ... & Chen, Y. (2020). MLL1 promotes myogenesis by epigenetically regulating Myf5. Cell Proliferation, 53(2), e12744.

Metin Kiyici, J., Arslan, K., Akyuz, B., Kaliber, M., Aksel, E. G., & Çinar, M. U. (2019). Relationships between polymorphisms of growth hormone, leptin and myogenic factor 5 genes with some milk yield traits in Holstein dairy cows. International journal of dairy technology, 72(1), 1-7.

Yoo, Y. M., Jung, E. M., & Jeung, E. B. (2019). Rapamycin-induced autophagy decreases Myf5 and MyoD proteins in C2C12 myoblast cells. Toxicology In Vitro, 58, 132-141.

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For research use only. Not intended for any clinical use.

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