Human MYF5 ELISA Kit (V2LY-0626-LY3551)

Name: Human MYF5 ELISA Kit
SKU: V2LY-0626-LY3551
Rating: 5 (1 reviews)

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Tested Data

Datasheet

SDS

Request for COA

Datasheet Target References Q & As Review & reward Protocols Associated Products

Basic Information

Sensitivity

0.02 ng/mL

Detection Range

0.05-10 ng/mL

Sample Type

Serum, Plasma, cell culture supernates

Specificity

Human

Assay Type

Sandwich

Reactivity

Human

Assay Time

1.5 h

Molecule Mass

28.3 kDa

Components

Pre-coated ELISA Plate: 12 wells * 8 detachable strips
Standard solution: 0.5ml x1
Standard diluent: 3ml x1
Streptavidin-HRP: 6ml x1
Stop solution: 6ml x1
Substrate solution A: 6ml x1
Substrate solution B: 6ml x1
Wash buffer concentrate (25x): 20ml x1
Biotinylated antibody: 1ml x1

Formulations & Storage [For reference only, actual COA shall prevail!]

Storage

Store at 2-8°C

More Infomation

Target

Full Name

MYOGENIC FACTOR 5

Function

Transcriptional activator that promotes transcription of muscle-specific target genes and plays a role in muscle differentiation (PubMed:29887215).

Together with MYOG and MYOD1, co-occupies muscle-specific gene promoter core region during myogenesis. Induces fibroblasts to differentiate into myoblasts. Probable sequence specific DNA-binding protein.

Biological Process

Camera-type eye development Source: Ensembl
Cartilage condensation Source: Ensembl
Embryonic skeletal system morphogenesis Source: Ensembl
Extracellular matrix organization Source: Ensembl
Muscle cell fate commitment Source: BHF-UCL
Muscle organ development Source: ProtInc
Muscle tissue morphogenesis Source: Ensembl
Ossification Source: Ensembl
Positive regulation of myoblast differentiation Source: GO_Central
Positive regulation of skeletal muscle fiber development Source: GO_Central
Regulation of cell-matrix adhesion Source: Ensembl
Regulation of transcription by RNA polymerase II Source: GO_Central
Skeletal muscle cell differentiation Source: GO_Central
Skeletal muscle tissue development Source: ProtInc
Somitogenesis Source: Ensembl

Cellular Location

Nucleus

Involvement in disease

Ophthalmoplegia, external, with rib and vertebral anomalies (EORVA):
An autosomal recessive disorder characterized by congenital nonprogressive external ophthalmoplegia, ptosis, scoliosis, torticollis, and vertebral and rib anomalies.

Shi, L. L., Zhu, K. C., & Wang, H. L. (2022). Characterization of myogenic regulatory factors, myod and myf5 from Megalobrama amblycephala and the effect of lipopolysaccharide on satellite cells in skeletal muscle. Gene, 834, 146608.

Dong, L., Wang, M., Gao, X., Zheng, X., Zhang, Y., Sun, L., ... & Wang, Y. (2022). miR-9-5p promotes myogenic differentiation via the Dlx3/Myf5 axis. PeerJ, 10, e13360.

Maeng, G., Das, S., Greising, S. M., Gong, W., Singh, B. N., Kren, S., ... & Garry, M. G. (2021). Humanized skeletal muscle in MYF5/MYOD/MYF6-null pig embryos. Nature Biomedical Engineering, 5(8), 805-814.

Zhao, C., Raza, S. H. A., Khan, R., Sabek, A., Khan, S., Ullah, I., ... & Zan, L. (2020). Genetic variants in MYF5 affected growth traits and beef quality traits in Chinese Qinchuan cattle. Genomics, 112(4), 2804-2812.

Osborn, D. P., Li, K., Cutty, S. J., Nelson, A. C., Wardle, F. C., Hinits, Y., & Hughes, S. M. (2020). Fgf-driven Tbx protein activities directly induce myf5 and myod to initiate zebrafish myogenesis. Development, 147(8), dev184689.

Cai, S., Zhu, Q., Guo, C., Yuan, R., Zhang, X., Nie, Y., ... & Chen, Y. (2020). MLL1 promotes myogenesis by epigenetically regulating Myf5. Cell Proliferation, 53(2), e12744.

Metin Kiyici, J., Arslan, K., Akyuz, B., Kaliber, M., Aksel, E. G., & Çinar, M. U. (2019). Relationships between polymorphisms of growth hormone, leptin and myogenic factor 5 genes with some milk yield traits in Holstein dairy cows. International journal of dairy technology, 72(1), 1-7.

Yoo, Y. M., Jung, E. M., & Jeung, E. B. (2019). Rapamycin-induced autophagy decreases Myf5 and MyoD proteins in C2C12 myoblast cells. Toxicology In Vitro, 58, 132-141.

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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme Linked Immunospot (ELISpot)
Proteogenomic
Other Protocols

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Mouse Anti-MYF5 Recombinant Antibody (CBFYM-2908) (CAT#: CBMAB-M3103-FY)

Isotype control

For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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