Rabbit Anti-NAE1 Recombinant Antibody (D9I4Z) (CBMAB-N1072-WJ)

Name: Rabbit Anti-NAE1 Recombinant Antibody (D9I4Z)
SKU: CBMAB-N1072-WJ
Rating: 5 (1 reviews)

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Datasheet

SDS

Request for COA

Datasheet Target References Q & As Review & reward Protocols Associated Products

Basic Information

Host Animal

Rabbit

Clone

D9I4Z

Application

Specificity

Human, Mouse, Rat, Monkey, Cattle, Dog

Antibody Isotype

IgG

Clonality

Monoclonal

Application Notes

The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Formulations & Storage [For reference only, actual COA shall prevail!]

Storage

Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

More Infomation

Target

Full Name

NEDD8 activating enzyme E1 subunit 1

Introduction

The protein encoded by this gene binds to the beta-amyloid precursor protein. Beta-amyloid precursor protein is a cell surface protein with signal-transducing properties, and it is thought to play a role in the pathogenesis of Alzheimer's disease. In addition, the encoded protein can form a heterodimer with UBE1C and bind and activate NEDD8, a ubiquitin-like protein. This protein is required for cell cycle progression through the S/M checkpoint. Three transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008]

Entrez Gene ID

Human	8883
Mouse	234664
Rat	84019
Monkey	696429
Cattle	614491
Dog	610026

UniProt ID

Human	Q13564
Mouse	Q8VBW6
Rat	Q9Z1A5
Monkey	H9G1D3
Cattle	E1B8X4
Dog	E2RE24

Alternative Names

NEDD8 Activating Enzyme E1 Subunit 1; Amyloid Beta Precursor Protein-Binding Protein 1, 59 KDa; Amyloid Beta Precursor Protein Binding Protein 1, 59kDa; Amyloid Protein-Binding Protein 1; Proto-Oncogene Protein 1; APP-BP1; APPBP1; Amyloid Beta Precursor Protein-Binding Protein 1, 59kD;

Function

Regulatory subunit of the dimeric UBA3-NAE1 E1 enzyme. E1 activates NEDD8 by first adenylating its C-terminal glycine residue with ATP, thereafter linking this residue to the side chain of the catalytic cysteine, yielding a NEDD8-UBA3 thioester and free AMP. E1 finally transfers NEDD8 to the catalytic cysteine of UBE2M. Necessary for cell cycle progression through the S-M checkpoint. Overexpression of NAE1 causes apoptosis through deregulation of NEDD8 conjugation.

Biological Process

Mitotic DNA replication checkpoint signaling Source: UniProtKB
Neuron apoptotic process Source: UniProtKB
Protein modification by small protein conjugation Source: GO_Central
Protein neddylation Source: UniProtKB
Regulation of apoptotic process Source: UniProtKB
Regulation of neuron apoptotic process Source: UniProtKB
Signal transduction Source: ProtInc

Cellular Location

Cell membrane
Note: Colocalizes with APP in lipid rafts.

PTM

Ubiquitinated by TRIP12, leading to its degradation by the proteasome.

Fu, D. J., & Wang, T. (2023). Targeting NEDD8-activating enzyme for cancer therapy: developments, clinical trials, challenges and future research directions. Journal of Hematology & Oncology, 16(1), 87.

Zhou, L. N., Xiong, C., Cheng, Y. J., Song, S. S., Bao, X. B., Huan, X. J., ... & He, J. X. (2022). SOMCL-19-133, a novel, selective, and orally available inhibitor of NEDD8-activating enzyme (NAE) for cancer therapy. Neoplasia, 32, 100823.

Olaizola, P., Lee-Law, P. Y., Fernandez-Barrena, M. G., Alvarez, L., Cadamuro, M., Azkargorta, M., ... & Banales, J. M. (2022). Targeting NAE1-mediated protein hyper-NEDDylation halts cholangiocarcinogenesis and impacts on tumor-stroma crosstalk in experimental models. Journal of Hepatology, 77(1), 177-190.

Barghout, S. H., & Schimmer, A. D. (2021). E1 enzymes as therapeutic targets in cancer. Pharmacological reviews, 73(1), 1-58.

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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme Linked Immunospot (ELISpot)
Proteogenomic
Other Protocols

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Isotype control

For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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