Human Recombinant NAE1 protein, His Tag (V2LY-0526-LY5687)

Name: Human Recombinant NAE1 protein, His Tag
SKU: V2LY-0526-LY5687
Rating: 5 (1 reviews)

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Datasheet

SDS

Request for COA

Datasheet Target References Q & As Review & reward Protocols Associated Products

Basic Information

Expressed Host

Baculovirus-Insect Cells

Protein Species

Human

Tag

His Tag

Protein Construction

This product is Human Recombinant NAE1 protein, His Tag consist of Amino Acid: 2-end and predicts a molecular mass of 65 kDa.

Molecule Mass

65 kDa

Sequence

Amino Acid: 2-end

Species

Human

Formulations & Storage [For reference only, actual COA shall prevail!]

Purity

Batch dependent.

Endotoxin

Please contact us for more information.

Format

Liquid

Buffer

Please contact us for more information.

Preservative

None

Storage

Store product at -70°C. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles.

More Infomation

Target

Full Name

NEDD8 activating enzyme E1 subunit 1

Function

Regulatory subunit of the dimeric UBA3-NAE1 E1 enzyme. E1 activates NEDD8 by first adenylating its C-terminal glycine residue with ATP, thereafter linking this residue to the side chain of the catalytic cysteine, yielding a NEDD8-UBA3 thioester and free AMP. E1 finally transfers NEDD8 to the catalytic cysteine of UBE2M. Necessary for cell cycle progression through the S-M checkpoint. Overexpression of NAE1 causes apoptosis through deregulation of NEDD8 conjugation.

Biological Process

Mitotic DNA replication checkpoint signaling Source: UniProtKB
Neuron apoptotic process Source: UniProtKB
Protein modification by small protein conjugation Source: GO_Central
Protein neddylation Source: UniProtKB
Regulation of apoptotic process Source: UniProtKB
Regulation of neuron apoptotic process Source: UniProtKB
Signal transduction Source: ProtInc

Cellular Location

Cell membrane
Note: Colocalizes with APP in lipid rafts.

PTM

Ubiquitinated by TRIP12, leading to its degradation by the proteasome.

Fu, D. J., & Wang, T. (2023). Targeting NEDD8-activating enzyme for cancer therapy: developments, clinical trials, challenges and future research directions. Journal of Hematology & Oncology, 16(1), 87.

Zhou, L. N., Xiong, C., Cheng, Y. J., Song, S. S., Bao, X. B., Huan, X. J., ... & He, J. X. (2022). SOMCL-19-133, a novel, selective, and orally available inhibitor of NEDD8-activating enzyme (NAE) for cancer therapy. Neoplasia, 32, 100823.

Olaizola, P., Lee-Law, P. Y., Fernandez-Barrena, M. G., Alvarez, L., Cadamuro, M., Azkargorta, M., ... & Banales, J. M. (2022). Targeting NAE1-mediated protein hyper-NEDDylation halts cholangiocarcinogenesis and impacts on tumor-stroma crosstalk in experimental models. Journal of Hepatology, 77(1), 177-190.

Barghout, S. H., & Schimmer, A. D. (2021). E1 enzymes as therapeutic targets in cancer. Pharmacological reviews, 73(1), 1-58.

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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme Linked Immunospot (ELISpot)
Proteogenomic
Other Protocols

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Isotype control

For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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