Mouse Anti-NAMPT Recombinant Antibody (CBT994) (V2LY-0625-LY3116)

Name: Mouse Anti-NAMPT Recombinant Antibody (CBT994)
SKU: V2LY-0625-LY3116
Rating: 5 (1 reviews)

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Tested Data

Mouse Anti-NAMPT Recombinant Antibody (CBT994) (V2LY-0625-LY3116) in WB

Datasheet

SDS

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Datasheet Target References Q & As Review & reward Protocols Associated Products

Basic Information

Host Animal

Mouse

Clone

CBT994

Application

WB, ELISA

Immunogen

Purified recombinant fragment of PBEF expressed in E. Coli.

Host Species

Mouse

Specificity

Human

Antibody Isotype

IgG

Clonality

Monoclonal Antibody

Application Notes

Application	Note
ELISA	1:10,000
WB	1:500-1:2,000

Formulations & Storage [For reference only, actual COA shall prevail!]

Format

Supernatant

Buffer

BSA, Glycerol

Preservative

Sodium azide

Concentration

Batch dependent

Storage

Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freezethaw cycles.

More Infomation

Target

Full Name

Nicotinamide Phosphoribosyltransferase

Entrez Gene ID

10135

UniProt ID

P43490

Function

Catalyzes the condensation of nicotinamide with 5-phosphoribosyl-1-pyrophosphate to yield nicotinamide mononucleotide, an intermediate in the biosynthesis of NAD. It is the rate limiting component in the mammalian NAD biosynthesis pathway. The secreted form behaves both as a cytokine with immunomodulating properties and an adipokine with anti-diabetic properties, it has no enzymatic activity, partly because of lack of activation by ATP, which has a low level in extracellular space and plasma. Plays a role in the modulation of circadian clock function. NAMPT-dependent oscillatory production of NAD regulates oscillation of clock target gene expression by releasing the core clock component: CLOCK-ARNTL/BMAL1 heterodimer from NAD-dependent SIRT1-mediated suppression (By similarity).

Biological Process

Cell-cell signaling Source: ProtInc
Circadian regulation of gene expression Source: UniProtKB
NAD biosynthesis via nicotinamide riboside salvage pathway Source: Reactome
NAD biosynthetic process Source: GO_Central
Positive regulation of cell population proliferation Source: ProtInc
Positive regulation of nitric-oxide synthase biosynthetic process Source: BHF-UCL
Positive regulation of transcription by RNA polymerase II Source: BHF-UCL
Signal transduction Source: ProtInc

Cellular Location

Nucleus
Cytoplasm
Secreted
Note: Under non-inflammatory conditions, visfatin predominantly exhibits a granular pattern within the nucleus. Secreted by endothelial cells upon IL-1beta stimulation. Abundantly secreted in milk, reaching 100-fold higher concentrations compared to maternal serum.

Gasparrini, M., & Audrito, V. (2022). NAMPT: A critical driver and therapeutic target for cancer. The International Journal of Biochemistry & Cell Biology, 145, 106189.

Wei, Y., Xiang, H., & Zhang, W. (2022). Review of various NAMPT inhibitors for the treatment of cancer. Frontiers in Pharmacology, 13, 970553.

Ratnayake, D., Nguyen, P. D., Rossello, F. J., Wimmer, V. C., Tan, J. L., Galvis, L. A., ... & Currie, P. D. (2021). Macrophages provide a transient muscle stem cell niche via NAMPT secretion. Nature, 591(7849), 281-287.

Oka, S. I., Byun, J., Huang, C. Y., Imai, N., Ralda, G., Zhai, P., ... & Sadoshima, J. (2021). Nampt potentiates antioxidant defense in diabetic cardiomyopathy. Circulation research, 129(1), 114-130.

Heske, C. M. (2020). Beyond energy metabolism: exploiting the additional roles of NAMPT for cancer therapy. Frontiers in oncology, 9, 1514.

Galli, U., Colombo, G., Travelli, C., Tron, G. C., Genazzani, A. A., & Grolla, A. A. (2020). Recent advances in NAMPT inhibitors: a novel immunotherapic strategy. Frontiers in pharmacology, 11, 656.

Audrito, V., Messana, V. G., & Deaglio, S. (2020). NAMPT and NAPRT: two metabolic enzymes with key roles in inflammation. Frontiers in Oncology, 10, 358.

Zhang, H., Zhang, N., Liu, Y., Su, P., Liang, Y., Li, Y., ... & Yang, Q. (2019). Epigenetic regulation of NAMPT by NAMPT-AS drives metastatic progression in triple-negative breast cancer. Cancer research, 79(13), 3347-3359.

Gardell, S. J., Hopf, M., Khan, A., Dispagna, M., Hampton Sessions, E., Falter, R., ... & Pinkerton, A. B. (2019). Boosting NAD+ with a small molecule that activates NAMPT. Nature communications, 10(1), 3241.

Wang, S. N., & Miao, C. Y. (2019). Targeting NAMPT as a therapeutic strategy against stroke. Stroke and vascular neurology, svn-2018.

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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme Linked Immunospot (ELISpot)
Proteogenomic
Other Protocols

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Isotype control

For research use only. Not intended for any clinical use.

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We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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