Rat Nampt ELISA Kit (V2LY-0626-LY900)

Name: Rat Nampt ELISA Kit
SKU: V2LY-0626-LY900
Rating: 5 (1 reviews)

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Tested Data

Datasheet

SDS

Request for COA

Datasheet Target References Q & As Review & reward Protocols Associated Products

Basic Information

Sensitivity

0.0025 ng/mL

Detection Range

0.005-2 ng/mL

Sample Type

Serum, Plasma, Urine, Ascites, Cerebrospinal fluid, Tissue

Specificity

Rat

Assay Type

Sandwich

Reactivity

Rat

Assay Time

1.5 h

Molecule Mass

55.4 kDa

Components

Standard Solution: 0.5ml x1
Pre-coated ELISA Plate: 12 wells * 8 detachable strips
Standard Diluent: 3ml x1
Streptavidin-HRP: 6ml x1
Stop Solution: 6ml x1
Substrate Solution A: 6ml x1
Substrate Solution B: 6ml x1
Wash Buffer Concentrate (25x): 20ml x1
Biotinylated Antibody: 1ml x1

Formulations & Storage [For reference only, actual COA shall prevail!]

Storage

Store at 2-8°C

More Infomation

Target

Full Name

Nicotinamide Phosphoribosyltransferase

Function

Catalyzes the condensation of nicotinamide with 5-phosphoribosyl-1-pyrophosphate to yield nicotinamide mononucleotide, an intermediate in the biosynthesis of NAD. It is the rate limiting component in the mammalian NAD biosynthesis pathway. The secreted form behaves both as a cytokine with immunomodulating properties and an adipokine with anti-diabetic properties, it has no enzymatic activity, partly because of lack of activation by ATP, which has a low level in extracellular space and plasma. Plays a role in the modulation of circadian clock function. NAMPT-dependent oscillatory production of NAD regulates oscillation of clock target gene expression by releasing the core clock component: CLOCK-ARNTL/BMAL1 heterodimer from NAD-dependent SIRT1-mediated suppression (By similarity).

Biological Process

Cell-cell signaling Source: ProtInc
Circadian regulation of gene expression Source: UniProtKB
NAD biosynthesis via nicotinamide riboside salvage pathway Source: Reactome
NAD biosynthetic process Source: GO_Central
Positive regulation of cell population proliferation Source: ProtInc
Positive regulation of nitric-oxide synthase biosynthetic process Source: BHF-UCL
Positive regulation of transcription by RNA polymerase II Source: BHF-UCL
Signal transduction Source: ProtInc

Cellular Location

Nucleus
Cytoplasm
Secreted
Note: Under non-inflammatory conditions, visfatin predominantly exhibits a granular pattern within the nucleus. Secreted by endothelial cells upon IL-1beta stimulation. Abundantly secreted in milk, reaching 100-fold higher concentrations compared to maternal serum.

Gasparrini, M., & Audrito, V. (2022). NAMPT: A critical driver and therapeutic target for cancer. The International Journal of Biochemistry & Cell Biology, 145, 106189.

Wei, Y., Xiang, H., & Zhang, W. (2022). Review of various NAMPT inhibitors for the treatment of cancer. Frontiers in Pharmacology, 13, 970553.

Ratnayake, D., Nguyen, P. D., Rossello, F. J., Wimmer, V. C., Tan, J. L., Galvis, L. A., ... & Currie, P. D. (2021). Macrophages provide a transient muscle stem cell niche via NAMPT secretion. Nature, 591(7849), 281-287.

Oka, S. I., Byun, J., Huang, C. Y., Imai, N., Ralda, G., Zhai, P., ... & Sadoshima, J. (2021). Nampt potentiates antioxidant defense in diabetic cardiomyopathy. Circulation research, 129(1), 114-130.

Heske, C. M. (2020). Beyond energy metabolism: exploiting the additional roles of NAMPT for cancer therapy. Frontiers in oncology, 9, 1514.

Galli, U., Colombo, G., Travelli, C., Tron, G. C., Genazzani, A. A., & Grolla, A. A. (2020). Recent advances in NAMPT inhibitors: a novel immunotherapic strategy. Frontiers in pharmacology, 11, 656.

Audrito, V., Messana, V. G., & Deaglio, S. (2020). NAMPT and NAPRT: two metabolic enzymes with key roles in inflammation. Frontiers in Oncology, 10, 358.

Zhang, H., Zhang, N., Liu, Y., Su, P., Liang, Y., Li, Y., ... & Yang, Q. (2019). Epigenetic regulation of NAMPT by NAMPT-AS drives metastatic progression in triple-negative breast cancer. Cancer research, 79(13), 3347-3359.

Gardell, S. J., Hopf, M., Khan, A., Dispagna, M., Hampton Sessions, E., Falter, R., ... & Pinkerton, A. B. (2019). Boosting NAD+ with a small molecule that activates NAMPT. Nature communications, 10(1), 3241.

Wang, S. N., & Miao, C. Y. (2019). Targeting NAMPT as a therapeutic strategy against stroke. Stroke and vascular neurology, svn-2018.

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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme Linked Immunospot (ELISpot)
Proteogenomic
Other Protocols

Alternative Versions

Human NAMPT ELISA Kit (CAT#: V2LY-0626-LY182)

Human NAMPT ELISA Kit (2) (CAT#: V2LY-0626-LY3910)

Human Recombinant NAMPT protein, GST Tag (CAT#: V2LY-0526-LY5690)

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Mouse Anti-NAMPT Recombinant Antibody (CBT994) (CAT#: V2LY-0625-LY3116)

Human NAMPT ELISA Kit (2) (CAT#: V2LY-0626-LY3910)

Rat Anti-NAMPT Recombinant Antibody (CBYC-P111) (CAT#: CBMAB-P0526-YC)

Rabbit Anti-NAMPT Recombinant Antibody (21HCLC) (CAT#: CBMAB-N1096-WJ)

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Mouse Anti-NAMPT Monoclonal Antibody (4D5) (CAT#: CBMAB-1712-YC)

Isotype control

For research use only. Not intended for any clinical use.

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