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Mouse Anti-NANOG Recombinant Antibody (2C4) (CBMAB-N1110-WJ)

This product is a Mouse antibody that recognizes NANOG. The antibody 2C4 can be used for immunoassay techniques such as: ELISA, FC, IF, IHC, WB.
See all NANOG antibodies

Summary

Host Animal
Mouse
Specificity
Human
Clone
2C4
Antibody Isotype
IgG
Application
ELISA, FC, IF, IHC, WB

Basic Information

Specificity
Human
Antibody Isotype
IgG
Clonality
Monoclonal
Application Notes
The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Formulations & Storage [For reference only, actual COA shall prevail!]

Format
Liquid
Buffer
PBS, pH 7.4
Preservative
0.02% sodium azide
Concentration
1 mg/mL
Storage
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

Target

Full Name
Nanog Homeobox
Introduction
The protein encoded by this gene is a DNA binding homeobox transcription factor involved in embryonic stem (ES) cell proliferation, renewal, and pluripotency. The encoded protein can block ES cell differentiation and can also autorepress its own expression in differentiating cells. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Sep 2015]
Entrez Gene ID
79923
UniProt ID
Q9H9S0
Alternative Names
Nanog Homeobox; Homeobox Transcription Factor Nanog; HNanog;
Function
Transcription regulator involved in inner cell mass and embryonic stem (ES) cells proliferation and self-renewal. Imposes pluripotency on ES cells and prevents their differentiation towards extraembryonic endoderm and trophectoderm lineages. Blocks bone morphogenetic protein-induced mesoderm differentiation of ES cells by physically interacting with SMAD1 and interfering with the recruitment of coactivators to the active SMAD transcriptional complexes. Acts as a transcriptional activator or repressor. Binds optimally to the DNA consensus sequence 5'-TAAT[GT][GT]-3' or 5'-[CG][GA][CG]C[GC]ATTAN[GC]-3'. Binds to the POU5F1/OCT4 promoter (PubMed:25825768).

Able to autorepress its expression in differentiating (ES) cells: binds to its own promoter following interaction with ZNF281/ZFP281, leading to recruitment of the NuRD complex and subsequent repression of expression. When overexpressed, promotes cells to enter into S phase and proliferation.
Biological Process
Cell differentiation Source: HGNC-UCL
Endodermal cell fate specification Source: MGI
Positive regulation of stem cell proliferation Source: HGNC-UCL
Positive regulation of transcription by RNA polymerase II Source: MGI
Regulation of cell differentiation Source: HGNC-UCL
Regulation of gene expression Source: UniProtKB
Regulation of transcription, DNA-templated Source: HGNC-UCL
Regulation of transcription by RNA polymerase II Source: GO_Central
Somatic stem cell population maintenance Source: UniProtKB
Stem cell population maintenance Source: BHF-UCL
Cellular Location
Nucleus

Kuznetsova, K., Chabot, N. M., Ugolini, M., Wu, E., Lalit, M., Oda, H., ... & Vastenhouw, N. L. (2023). Nanog organizes transcription bodies. Current Biology, 33(1), 164-173.

Vasefifar, P., Motafakkerazad, R., Maleki, L. A., Najafi, S., Ghrobaninezhad, F., Najafzadeh, B., ... & Baradaran, B. (2022). Nanog, as a key cancer stem cell marker in tumor progression. Gene, 827, 146448.

Yoon, C., Lu, J., Yi, B. C., Chang, K. K., Simon, M. C., Ryeom, S., & Yoon, S. S. (2021). PI3K/Akt pathway and Nanog maintain cancer stem cells in sarcomas. Oncogenesis, 10(1), 12.

Najafzadeh, B., Asadzadeh, Z., Motafakker Azad, R., Mokhtarzadeh, A., Baghbanzadeh, A., Alemohammad, H., ... & Baradaran, B. (2021). The oncogenic potential of NANOG: An important cancer induction mediator. Journal of cellular physiology, 236(4), 2443-2458.

Springer, C., Zakhartchenko, V., Wolf, E., & Simmet, K. (2021). Hypoblast formation in bovine embryos does not depend on NANOG. Cells, 10(9), 2232.

Fatma, H., Siddique, H. R., & Maurya, S. K. (2021). The multiple faces of NANOG in cancer: a therapeutic target to chemosensitize therapy-resistant cancers. Epigenomics, 13(23), 1885-1900.

Grubelnik, G., Boštjančič, E., Pavlič, A., Kos, M., & Zidar, N. (2020). NANOG expression in human development and cancerogenesis. Experimental Biology and Medicine, 245(5), 456-464.

Grubelnik, G., Boštjančič, E., Grošelj, A., & Zidar, N. (2020). Expression of NANOG and its regulation in oral squamous cell carcinoma. BioMed Research International, 2020.

Mahalaxmi, I., Devi, S. M., Kaavya, J., Arul, N., Balachandar, V., & Santhy, K. S. (2019). New insight into NANOG: A novel therapeutic target for ovarian cancer (OC). European journal of pharmacology, 852, 51-57.

Heurtier, V., Owens, N., Gonzalez, I., Mueller, F., Proux, C., Mornico, D., ... & Navarro, P. (2019). The molecular logic of Nanog-induced self-renewal in mouse embryonic stem cells. Nature communications, 10(1), 1109.

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For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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