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Mouse Anti-NANOG Recombinant Antibody (CBT936) (V2LY-0625-LY3169)


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Tested Data
Mouse Anti-NANOG Recombinant Antibody (CBT936) (V2LY-0625-LY3169) in IF
Mouse Anti-NANOG Recombinant Antibody (CBT936) (V2LY-0625-LY3169) in IF
Mouse Anti-NANOG Recombinant Antibody (CBT936) (V2LY-0625-LY3169) in WB
Mouse Anti-NANOG Recombinant Antibody (CBT936) (V2LY-0625-LY3169) in WB

Summary

Host Animal
Mouse
Specificity
Human
Clone
CBT936
Antibody Isotype
IgG
Application
WB, IF, ICC, ELISA

Basic Information

Immunogen
Purified recombinant fragment of Nanog (aa20-166) expressed in E. Coli.
Host Species
Mouse
Specificity
Human
Antibody Isotype
IgG
Clonality
Monoclonal Antibody
Application Notes
ApplicationNote
ELISA1:10,000
IF1:200-1:1,000
WB1:500-1:2,000

Formulations & Storage [For reference only, actual COA shall prevail!]

Format
Supernatant
Buffer
BSA, Glycerol
Preservative
Sodium azide
Concentration
Batch dependent
Storage
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freezethaw cycles.

Target

Entrez Gene ID
79923
UniProt ID
Q9H9S0
Function
Transcription regulator involved in inner cell mass and embryonic stem (ES) cells proliferation and self-renewal. Imposes pluripotency on ES cells and prevents their differentiation towards extraembryonic endoderm and trophectoderm lineages. Blocks bone morphogenetic protein-induced mesoderm differentiation of ES cells by physically interacting with SMAD1 and interfering with the recruitment of coactivators to the active SMAD transcriptional complexes. Acts as a transcriptional activator or repressor. Binds optimally to the DNA consensus sequence 5'-TAAT[GT][GT]-3' or 5'-[CG][GA][CG]C[GC]ATTAN[GC]-3'. Binds to the POU5F1/OCT4 promoter (PubMed:25825768).

Able to autorepress its expression in differentiating (ES) cells: binds to its own promoter following interaction with ZNF281/ZFP281, leading to recruitment of the NuRD complex and subsequent repression of expression. When overexpressed, promotes cells to enter into S phase and proliferation.
Biological Process
Cell differentiation Source: HGNC-UCL
Endodermal cell fate specification Source: MGI
Positive regulation of stem cell proliferation Source: HGNC-UCL
Positive regulation of transcription by RNA polymerase II Source: MGI
Regulation of cell differentiation Source: HGNC-UCL
Regulation of gene expression Source: UniProtKB
Regulation of transcription, DNA-templated Source: HGNC-UCL
Regulation of transcription by RNA polymerase II Source: GO_Central
Somatic stem cell population maintenance Source: UniProtKB
Stem cell population maintenance Source: BHF-UCL
Cellular Location
Nucleus
More Infomation

Kuznetsova, K., Chabot, N. M., Ugolini, M., Wu, E., Lalit, M., Oda, H., ... & Vastenhouw, N. L. (2023). Nanog organizes transcription bodies. Current Biology, 33(1), 164-173.

Vasefifar, P., Motafakkerazad, R., Maleki, L. A., Najafi, S., Ghrobaninezhad, F., Najafzadeh, B., ... & Baradaran, B. (2022). Nanog, as a key cancer stem cell marker in tumor progression. Gene, 827, 146448.

Yoon, C., Lu, J., Yi, B. C., Chang, K. K., Simon, M. C., Ryeom, S., & Yoon, S. S. (2021). PI3K/Akt pathway and Nanog maintain cancer stem cells in sarcomas. Oncogenesis, 10(1), 12.

Najafzadeh, B., Asadzadeh, Z., Motafakker Azad, R., Mokhtarzadeh, A., Baghbanzadeh, A., Alemohammad, H., ... & Baradaran, B. (2021). The oncogenic potential of NANOG: An important cancer induction mediator. Journal of cellular physiology, 236(4), 2443-2458.

Springer, C., Zakhartchenko, V., Wolf, E., & Simmet, K. (2021). Hypoblast formation in bovine embryos does not depend on NANOG. Cells, 10(9), 2232.

Fatma, H., Siddique, H. R., & Maurya, S. K. (2021). The multiple faces of NANOG in cancer: a therapeutic target to chemosensitize therapy-resistant cancers. Epigenomics, 13(23), 1885-1900.

Grubelnik, G., Boštjančič, E., Pavlič, A., Kos, M., & Zidar, N. (2020). NANOG expression in human development and cancerogenesis. Experimental Biology and Medicine, 245(5), 456-464.

Grubelnik, G., Boštjančič, E., Grošelj, A., & Zidar, N. (2020). Expression of NANOG and its regulation in oral squamous cell carcinoma. BioMed Research International, 2020.

Mahalaxmi, I., Devi, S. M., Kaavya, J., Arul, N., Balachandar, V., & Santhy, K. S. (2019). New insight into NANOG: A novel therapeutic target for ovarian cancer (OC). European journal of pharmacology, 852, 51-57.

Heurtier, V., Owens, N., Gonzalez, I., Mueller, F., Proux, C., Mornico, D., ... & Navarro, P. (2019). The molecular logic of Nanog-induced self-renewal in mouse embryonic stem cells. Nature communications, 10(1), 1109.

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For research use only. Not intended for any clinical use.

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